Terahertz chemical microscope for label-free detection of protein complex

Kiwa, Toshihiko; Kondo, Yousuke; Minami, Yuji; Kawayama, Iwao; Tonouchi, Masayoshi; Tsukada, Keiji
May 2010
Applied Physics Letters;5/24/2010, Vol. 96 Issue 21, p211114
Academic Journal
A terahertz (THz) chemical microscope (TCM) has been proposed and developed for label-free analysis of the interactions of proteins, including immune reactions. A SiO2/Si/sapphire plate was fabricated and emitted THz pulses from the sapphire side using a femtosecond laser. The amplitude of a THz pulse depends on the chemical potential of the SiO2 surface. As an initial demonstration, a biotin–avidin complex was analyzed using TCM. Results indicate that biotin concentrations of up to 10-12 mol/l could be detected, suggesting that a high sensitivity immunoassay system can be realized using this method. The distribution of the THz peak amplitude was measured, reflecting the distribution of the biotin–avidin complex on the plate.


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