TITLE

Optical recognition of salivary proteins by use of molecularly imprinted poly(ethylene- co-vinyl alcohol)/quantum dot composite nanoparticles

AUTHOR(S)
Mei-Hwa Lee; Yun-Chao Chen; Min-Hsien Ho; Hung-Yin Lin
PUB. DATE
June 2010
SOURCE
Analytical & Bioanalytical Chemistry;Jun2010, Vol. 397 Issue 4, p1457
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Molecularly imprinted polymers (MIPs) have long been studied for applications in biomolecule recognition and binding; compared with natural antibodies, they may offer advantages in cost and stability. We report on the development of MIPs that “self-report” concentrations of bound analytes via fluorescence changes in embedded quantum dots (QDots). Composite QDot/MIPs were prepared using phase inversion of poly(ethylene- co-vinyl alcohol) (EVAL) solutions with various ethylene mole ratios in the presence of salivary target molecules (e.g. amylase, lipase, and lysozyme). These major protein components of saliva have been implicated as possible biomarkers for pancreatic cancer. The optimum (highest imprinting effectiveness) ethylene mole ratios of the commercially available EVALs were found to be 32, 38, and 44 mol% for the imprinting of amylase, lipase, and lysozyme, respectively. QD fluorescence quenching was observed on binding of analytes to composite MIPs in a concentration-dependent manner, and was used to construct calibration curves. Finally, the composite MIP particles were used for the quantitative detection of amylase, lipase, and lysozyme in real samples (saliva) and compared with a commercial Architect ci 8200 chemical analysis system.
ACCESSION #
50870509

 

Related Articles

  • Surface-Enhanced Raman Nanoparticles for in-vivo Tumor Targeting and Spectroscopic Detection. Ximei Qian; Shuming Nie // AIP Conference Proceedings;8/6/2010, Vol. 1267 Issue 1, p81 

    We have developed a new class of biocompatible and nontoxic SERS nanoparticles for in-vivo tumor targeting and detection. A major finding is that small-molecule Raman reporters such as organic dyes are not displaced but are stabilized by thiol-modified polyethylene glycols. These pegylated SERS...

  • The Relationship Between Calculus Index and Protease Activity in Saliva. WATANABE, TATSUO; SHINMOTO, MASANA; TODA, KYOJI; MORISHITA, MASAYUKI; IWAMOTO, YOSHIFUMI // Journal of Dental Research;Feb1980, Vol. 59 Issue 2, p138 

    The article presents information on research into correlations between the protease activity present in saliva, age, and index of dental calculus. The methodology of an experiment involving the saliva of 77 adults is described, and a table of results is presented illustrating the positive and...

  • Estudio in vitro de algunos factores relacionados con la formación de película adquirida. Francia, Catalina Melchora; Lissera, Rosa Guadalupe; Battellino, Luis José // Medicina Oral (16656024);2004, Vol. 6 Issue 1, p5 

    An experimental study was carried out incubating to 37 °C for 90 min. a mixture of hydroxyapatite and supernatant (12.000 g 15 min.) of mixed saliva (20 mg/mL) to establish the possible influence of the availability of proteins, the pH, certain ions, the temperature and the ionic force on the...

  • Saliva Diagnostics Integrate Dentistry into General and Preventive Health Care. Mijin Choi // International Journal of Prosthodontics;2010, Vol. 23 Issue 3, p189 

    The author reflects on the use of saliva diagnostics in monitoring the health status of individuals. She states that saliva diagnosis has been studied for use in detecting diseases such as breast cancer, cystic fibrosis, diabetes mellitus, HIV, oral cancer, and more. She comments on the need to...

  • Human Mixed Saliva Protein Concentration. BONILLA, CARLOS A. // Journal of Dental Research;Mar1972, Vol. 51 Issue 2, p664 

    The article discusses a study analyzing the protein content of human mixed saliva. Samples of saliva were collected, centrifuged and measured for protein against crystalline bovine albumin. Samples were occasionally dialyzed against water for analysis and comparison. The author comments that the...

  • Saliva A Window to Health Status. Suszkiw, Jan // Agricultural Research;May2006, Vol. 54 Issue 5, p9 

    The article discusses research being done on human saliva. In a research project called salivary proteomics cataloging, scientists from the U.S. Agricultural Research Service, the University of Rochester and Scripps Research Institute are cataloging the thousands of proteins, lipids, sugars and...

  • The complete replacement of antibodies by protein-imprinted poly(ethylene-co-vinyl alcohol) in sandwich fluoroimmunoassays. Lee, Mei-Hwa; Thomas, James L.; Chen, Yun-Chao; Chin, Wei-Ti; Lin, Hung-Yin // Microchimica Acta;Nov2013, Vol. 180 Issue 15/16, p1393 

    The replacement of antibodies by molecularly imprinted polymers (MIPs) has been investigated for many decades. However, indirect protocols (including natural primary and secondary antibodies) are still utilized to evaluate the ability of MIP thin films to recognize target molecules. MIPs can be...

  • Fluorescent turn-on detection of cysteine using a molecularly imprinted polyacrylate linked to allylthiol-capped CdTe quantum dots. Chao, Mu-Rong; Hu, Chiung-Wen; Chen, Jian-Lian // Microchimica Acta;Jul2014, Vol. 181 Issue 9/10, p1085 

    CdTe quantum dots capped with thioglycolic acid (TGA) display a strong turn-on fluorescence response if exposed to solutions of cysteine (Cys). In order to exploit this effect, a molecularly imprinted polymer (MIP) for Cys was covalently linked to the QDs via allyl mercaptan. The resulting...

  • Neurotechniques: Quantum leap for quantum dots. Lopez, Juan Carlos // Nature Reviews Neuroscience;Mar2003, Vol. 4 Issue 3, p163 

    Focuses on studies which determined the use of quantum dots (QD) as tools for cellular imaging by reporting ways to circumvent problems with semiconductors. Information on QD; Details of QD labelling; Discussion on nanoparticles.

Share

Read the Article

Courtesy of VIRGINIA BEACH PUBLIC LIBRARY AND SYSTEM

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics