Focus on quantum dots as potential fluorescent probes for monitoring food toxicants and foodborne pathogens

Vinayaka, A. C.; Thakur, M. S.
June 2010
Analytical & Bioanalytical Chemistry;Jun2010, Vol. 397 Issue 4, p1445
Academic Journal
Water-soluble quantum dots (QDs) are fluorescent semiconductor nanoparticles with narrow, very specific, stable emission spectra. Therefore, the bioconjugation of these QDs for biological fluorescent labeling may be of interest due to their unique physical and optical properties as compared to organic fluorescent dyes. These intrinsic properties of QDs have been used for the sensitive detection of target analytes. From the viewpoint of ensuring food safety, there is a need to develop rapid, sensitive and specific detection techniques to monitor food toxicants in food and environmental samples. Even trace levels of these toxicants can inadvertently enter the food chain, creating severe health hazards. The present review emphasizes the application of water-soluble bioconjugated QDs for the detection of food contaminants such as pesticides, pathogenic bacterial toxins such as botulinum toxin, enterotoxins produced by Staphylococcus aureus, Escherichia coli, and for the development of oligonucleotide-based microarrays. This review also emphasizes the application of a possible resonance energy transfer phenomenon resulting from nanobiomolecular interactions obtained through the bioconjugation of QDs with biomolecules. Furthermore, the utilization of significant changes in the spectral behavior of QDs (attributed to resonance energy transfer in the bioconjugate) in future nanobiosensor development is also emphasized.


Related Articles

  • Imaging Escherichia coli using functionalized core/shell CdSe/CdS quantum dots. Hirschey, Matthew D.; Yong-Jin Han; Stucky, Galen D.; Butler, Alison // Journal of Biological Inorganic Chemistry;Sep2006, Vol. 11 Issue 5, p663 

    The internalization of a series of water-soluble CdSe/CdS quantum dots (QDs) stabilized by citrate, isocitrate, succinate, and malate by Escherichia coli is established by epifluorescence and confocal fluorescence scanning microscopy, fluorimetry, and UV�vis spectroscopy on whole and lysed...

  • Au:CdHgTe quantum dots for in vivo tumor-targeted multispectral fluorescence imaging. Han, Sihai; Mu, Ying; Zhu, Qiangyuan; Gao, Yibo; Li, Zuhong; Jin, Qinhan; Jin, Wei // Analytical & Bioanalytical Chemistry;Sep2012, Vol. 403 Issue 5, p1343 

    Near-infrared gold-doped CdHgTe quantum dots (QDs) with improved photoluminescence and biocompatibility were developed using an aqueous solution route with l-glutathione and l-cysteine as stabilizers. As-prepared Au:CdHgTe QDs were covalently linked to arginine-glycine-aspartic acid (RGD)...

  • Extracting fluorescence signal due to direct excitation of the energy acceptor from quantum dot-based FRET. Chaobiao Huang; Chuanliu Wu; Yibing Zhao // Journal of Nanoparticle Research;Aug2010, Vol. 12 Issue 6, p2153 

    An “in situ” strategy for extracting the fluorescence signal of dye acceptors due to direct excitation from Qdot-based FRET systems has been reported. The relevant theory model was developed to describe the present strategy. This strategy involves selective control of the quantum...

  • A CMOS Time-Resolved Fluorescence Lifetime Analysis Micro-System. Rae, Bruce R.; Muir, Keith R.; Zheng Gong; McKendry, Jonathan; Girkin, John M.; Gu, Erdan; Renshaw, David; Dawson, Martin D.; Henderson, Robert K. // Sensors (14248220);2009, Vol. 9 Issue 11, p9255 

    We describe a CMOS-based micro-system for time-resolved fluorescence lifetime analysis. It comprises a 16 × 4 array of single-photon avalanche diodes (SPADs) fabricated in 0.35 μm high-voltage CMOS technology with in-pixel time-gated photon counting circuitry and a second device...

  • Detecting Proteins in Highly Autofluorescent Cells Using Quantum Dot Antibody Conjugates. Orcutt, Karen M.; Ren, Shanshan; Gundersen, Kjell // Sensors (14248220);2009, Vol. 9 Issue 9, p7540 

    We have applied quantum dot (Qdot) antibody conjugates as a biomolecular probe for cellular proteins important in biogeochemical cycling in the sea. Conventional immunological methods have been hampered by the strong autofluorescence found in cyanobacteria cells. Qdot conjugates provide an ideal...

  • Red luminescence from strain-induced GaInP quantum dots. Sopanen, M.; Taskinen, M.; Lipsanen, H.; Ahopelto, J. // Applied Physics Letters;11/25/1996, Vol. 69 Issue 22, p3393 

    The strain of self-organized InP islands is used to induced quantum dots in near-surface GaInP/AlGaInP quantum wells. To obtain quantum dot luminescence in a widely tunable wavelength range of 630–700 nm, the composition and thickness of the GaInP quantum well is varied. The effect of...

  • Nonexponential "blinking" kinetics of single CdSe quantum dots: A universal power law behavior. Kuno, M.; Fromm, D. P.; Fromm, D.P.; Hamann, H. F.; Hamann, H.F.; Gallagher, A.; Nesbitt, D. J.; Nesbitt, D.J. // Journal of Chemical Physics;2/15/2000, Vol. 112 Issue 7 

    Single molecule confocal microscopy is used to study fluorescence intermittency of individual ZnS overcoated CdSe quantum dots (QDs) excited at 488 nm. The confocal apparatus permits the distribution of "on" and "off" times (i.e., periods of sustained fluorescence emission and darkness) to be...

  • Cranberry marinade improves the safety of chicken wings.  // Emerging Food R&D Report;Jan2012, Vol. 22 Issue 10, p5 

    The article discusses a study conducted by the researchers of the University of Maine in Maine, which investigates the ability of cranberry marinade to improve the safety of chicken wings. The researchers have studied the antimicrobial effects of a cranberry marinade against salmonella...

  • Rapid, Sensitive, and Simultaneous Detection of Three Foodborne Pathogens Using Magnetic Nanobead-Based Immunoseparation and Quantum Dot-Based Multiplex Immunoassay. Hong Wang; Yanbin Li; Wang, Andrew; Slavik, Michael // Journal of Food Protection;Dec2011, Vol. 74 Issue 12, p2039 

    Losses caused by foodborne diseases are enormous in terms of human life, illness, medical costs, and food product recalls. Rapid detection of multiple bacterial pathogens in foods is extremely important to ensure food safety. The objective of this research was to develop a multiplex immunoassay...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics