TITLE

Effect of enamel matrix proteins on the growth of apatite coating on chemically modified titanium

AUTHOR(S)
WANG Xiao-jie; HUANG Hui; YANG Fei; ZHANG Fu-qiang
PUB. DATE
February 2010
SOURCE
Shanghai Journal of Stomatology;Feb2010, Vol. 19 Issue 1, p49
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
PURPOSE: To evaluate the effects of enamel matrix proteins(EMPs) on the growth of apatite coatings on dual thermo-etching or alkali heat-treated pure titanium(Ti). METHODS: EMPs were extracted from porcine tooth germs and identified by SDS-PACE. Dual thermo-etching treated or alkali-heat treated Ti samples, then, were exposed in a blank or EMPs containing(150µg/mL) modified simulated body fluid(m-SBF) at 37°C for 7 days. SEM, EDS, XRD and FTIR were employed to observe or analyze the morphologies, element components and crystal structures of the apatite coatings. RESULTS: In dual thermo-etching-treated groups, almost no calcium-phosphate deposits were found on the titanium surfaces of control group and some amount of calcium-phosphate deposits with the main element components of Ca, P, 0 and C, however, formed on those of experimental group. But in alkali heat-treated groups, the apatite coatings proved to be carbonate hydroxyapatite formed on the titanium surfaces of both control group and experimental group, and the apatite coatings of the latter were found porous(300-600nm)and uneven. CONCLUSIONS: Alkali heat-treatment is in favor of apatite deposition, and EMPs can promote apatite deposition on both dual thermo-etching and alkali heat-treated pure titanium surfaces. Supported by National Natural Science Foundation of China(Grant No. 30670555), Shanghai Leading Academic Discipline Project (Grant No.S30206) and Research Fund of Science and Technology Commission of Shanghai Municipality(Grant No.08DZ2271100).
ACCESSION #
50596754

 

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