Droplet electric separator microfluidic device for cell sorting

Feng Guo; Xing-Hu Ji; Kan Liu; Rong-Xiang He; Li-Bo Zhao; Zhi-Xiao Guo; Wei Liu; Shi-Shang Guo; Xing-Zhong Zhao
May 2010
Applied Physics Letters;5/10/2010, Vol. 96 Issue 19, p193701
Academic Journal
A simple and effective droplet electric separator microfluidic device was developed for cell sorting. The aqueous droplet without precharging operation was influenced to move a distance in the channel along the electric field direction by applying dc voltage on the electrodes beside the channel, which made the target droplet flowing to the collector. Single droplet can be isolated in a sorting rate of ∼100 Hz with microelectrodes under a required pulse. Single or multiple mammalian cell (HePG2) encapsulated in the surfactant free alginate droplet could be sorted out respectively. This method may be used for single cell operation or analysis.


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