TITLE

Comparison of the mass transfer in totally porous and superficially porous stationary phases in liquid chromatography

AUTHOR(S)
Kiss, Ibolya; Bacskay, Ivett; Kilár, Ferenc; Felinger, Attila
PUB. DATE
June 2010
SOURCE
Analytical & Bioanalytical Chemistry;Jun2010, Vol. 397 Issue 3, p1307
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
The characterization of mass-transfer processes in a chromatographic column during a separation process is essential, since the influence of the mass-transfer kinetics on the shape of the chromatographic band profiles and on the efficiency of the separation is crucial. Several sources of mass transfer in a chromatographic bed have been identified and studied: the axial dispersion in the stream of mobile phase, the external mass-transfer resistance, intraparticle diffusion, and the kinetics of adsorption–desorption. We measured and compared the characteristics and performance of a new brand of shell particles and those of a conventional brand of totally porous silica particles. The shell stationary phase was made of 2.7-µm superficially porous particles (a 1.7-µm solid core is covered with a 0.5-µm-thick shell of porous silica). The other material consisted of totally porous particles of conventional 3.5-µm commercial silica. We measured the first and second central moments of the peaks of human insulin over a wide range of mobile phase velocities (from 0.02 to 1.3 mL/min) at 20°C. The plate height equations were constructed and the axial dispersion, external mass transfer, as well as the intraparticle diffusion coefficients were calculated for the two stationary phases.
ACCESSION #
50377648

 

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