Urinary Biomarkers for the Early Diagnosis of Kidney Cancer

Morrissey, Jeremiah J.; London, Amy N.; Jingqin Luo; Kharasch, Evan D.
May 2010
Mayo Clinic Proceedings;May2010, Vol. 85 Issue 5, p413
Academic Journal
OBJECTIVE: To test the hypothesis that increased tumor expression of proteins such as aquaporin-1 (AQP1) and adipophilin (ADFP) in patients with renal cancer would result in increased urine AQP1 and ADFP excretion. PATIENTS AND METHODS: Prenephrectomy and postnephrectomy (pseudocontrol) urine samples were collected from 42 patients with an incidental radiographically discovered renal mass and presurgical presumptive diagnosis of kidney cancer from July 8, 2008, through March 10, 2009. Also enrolled were 15 control patients who underwent nonrenal surgery and 19 healthy volunteers. Urine AQP1 and ADFP concentrations normalized to urine creatinine were determined by sensitive and specific Western blot assays. RESULTS: Mean ± SD preexcision urine AQPI and ADFP concentrations (76±29 and 117±74 arbitrary units, respectively) in patients with a pathologic diagnosis of clear ceil (n=22) or papillary (n=10) cancer were significantly greater than in patients with renal cancer of nonproximal tubule origin, control surgical patients, and healthy volunteers (combined values of 0.1±0.1 and 1.0±1.6 arbitrary units, respectively; n=44; P<.001). The AQPI and ADFP concentrations decreased 88% to 97% in the 25 patients with clear cell or papillary cancer who provided postnephrectomy follow-up urine samples. in patients with clear cell and papillary carcinoma, a linear correlation (Spearman) was found between tumor size and preexclsion urine AQPI or ADFP concentration (r=0.82 and 0.76, respectively; P<.001 for each). CONCLUSION: Urine AQPI and ADFP concentrations appear to be sensitive and specific biomarkers of kidney cancers of proximal tubule origin. These biomarkers may be useful to diagnose an Imaged renal mass and screen for kidney cancer at an early stage.


Related Articles

  • Urine 1,6-Hexamethylene Diamine (HDA) Levels Among Workers Exposed to 1,6-Hexamethylene Diisocyanate (HDI). Gaines, Linda G. T.; Fent, Kenneth W.; Flack, Sheila L.; Thomasen, Jennifer M.; Ball, Louise M.; Richardson, David B.; Ding, Kai; Whittaker, Stephen G.; Nylander-french, Leena A. // Annals of Occupational Hygiene;Aug2010, Vol. 54 Issue 6, p678 

    Urinary 1,6-hexamethylene diamine (HDA) may serve as a biomarker for systemic exposure to 1,6-hexamethylene diisocyanate (HDI) in occupationally exposed populations. However, the quantitative relationships between dermal and inhalation exposure to HDI and urine HDA levels have not been...

  • Association Between Lifetime Cadmium Intake and Cadmium Concentration in Individual Urine. Kobayashi, E.; Suwazono, Y.; Uetani, M.; Inaba, T.; Oishi, M.; Kido, T.; Nakagawa, H.; Nogawa, K. // Bulletin of Environmental Contamination & Toxicology;May2005, Vol. 74 Issue 5, p817 

    This article examines the relationships between calculated life-time cadmium (LCd) intake and the corresponding individual urinary cadmium concentration (UCd). UCd was expressed after correction with creatinine. The values of LCd and UCd were transformed to logarithmic values. Cadmium...

  • Urinary expression of acute kidney injury biomarkers in patients after RIRS: it is a prospective, controlled study. Dede, Onur; Dağguli, Mansur; Utanğaç, Mazhar; Bodakcı, Mehmet Nuri; Hatipoğlu, Namık Kemal; Sancaktutar, Ahmet Ali; Penbegül, Necmettin; Yuksel, Hatice // International Journal of Clinical & Experimental Medicine;2015, Vol. 8 Issue 5, p8147 

    Objective: To evaluated the damage effects of retrograde intra-renal surgery (RIRS) on kidney tissue by measuring kidney injury molecule-1 (KIM-1), neutrophil gelatinase-associated lipocalin (NGAL), N-acetyl-β-Dglucosaminidase (NAG), liver-type fatty acid binding protein (LFABP) expression....

  • Variable Effectiveness. Konkel, Lindsey // Environmental Health Perspectives;Feb2016, Vol. 124 Issue 2, pA37 

    The article discusses the variable effectiveness of standardization techniques in estimating the chemical exposure of human blood serum and urine. People are noted to vary in urinary diluteness and serum lipid levels due to recent water or dietary fat intake which may affect the accuracy of...

  • Determination of Urinary Biomarkers for Assessment of Short-Term Human Exposure to Aflatoxins in São Paulo, Brazil. Jager, Alessandra V.; Tonin, Fernando G.; Souto, Pollyana C. M. C.; Privatti, Rafaela T.; Oliveira, Carlos A. F. // Toxins;Jul2014, Vol. 6 Issue 7, p1996 

    In the present study, a longitudinal assessment was carried out to evaluate the short-term human exposure to aflatoxins in Pirassununga region, São Paulo, Brazil, by determination of urinary aflatoxins by a liquid chromatography coupled to mass sprectrometry (UPLC-MS/MS) method. Sixteen...

  • Posters - Quality Assurance.  // Annals of Clinical Biochemistry;Jan2006 Supplement, Vol. 43, p143 

    EQAS blunders - are we finding enough? M Davies, Leighton. 143 Vitamin D, A, E and alpha and beta carotenes: what are the best conditions for sample storage and transport? L T Ford, Birmingham. 143 Brighton-up your day with some urine EQA! R Marrington, Birmingham. 143 Initial experience of the...

  • Oliguria as predictive biomarker of acute kidney injury in critically ill patients.  // Critical Care;2011, Vol. 15 Issue 3, p10318 

    The article presents a study on the use of oliguria as biomarker of acute kidney injury (AKI). Serum creatinine concentration and urine output were documented and collected from among the AKI patients to define oliguria. The results of the study show that oliguria is a significant predictor of...

  • Plasma Citrate Levels as a Potential Biomarker for Glaucoma. Fraenkl, Stephan A.; Muser, Juergen; Groell, Rene; Reinhard, Graziella; Orgul, Selim; Flammer, Josef; Goldblum, David // Journal of Ocular Pharmacology & Therapeutics;Dec2011, Vol. 27 Issue 6, p577 

    Purpose: To determine the possibility of plasma citrate as a biomarker in patients with glaucoma. Methods: Twenty-one consecutive Caucasian patients with glaucoma and 21 sex- and age-matched controls were investigated. Plasma citrate, plasma creatinine, urine citrate, and urine creatinine were...

  • Developing Biomarkers for Methamphetamine Addiction. Mendelson, John; Baggott, Matthew J.; Flower, Keith; Galloway, Gantt // Current Neuropharmacology;Mar2011, Vol. 9 Issue 1, p100 

    No abstract available.


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics