Note: Dynamic point spread function for single and multiphoton fluorescence microscopy

Mondal, Partha Pratim; Mandal, Subhra; Diaspro, Alberto
April 2010
Review of Scientific Instruments;Apr2010, Vol. 81 Issue 4, p046103
Academic Journal
We propose and demonstrate a dynamic point spread function (PSF) for single and multiphoton fluorescence microscopy. The goal is to generate a PSF whose shape and size can be maneuvered from highly localized to elongated one, thereby allowing shallow-to-depth excitation capability during active imaging. The PSF is obtained by utilizing specially designed spatial filter and dynamically altering the filter parameters. We predict potential applications in nanobioimaging and fluorescence microscopy.


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