Generation of calcium waves in living cells by pulsed-laser-induced photodisruption

Smith, Nicholas I.; Fujita, Katsumasa; Kaneko, Tomoyuki; Katoh, Kentaro; Nakamura, Osamu; Kawata, Satoshi; Takamatsu, Tetsuro
August 2001
Applied Physics Letters;8/20/2001, Vol. 79 Issue 8, p1208
Academic Journal
Here, we present an optical technique that can induce waves of calcium ion concentration in live biological cells. Ca[sup 2+] waves were induced by femtosecond pulsed-laser illumination. Living HeLa cells were exposed to focused 140 fs pulses of 780 nm wavelength at 30 mW average power. Ca[sup 2+] waves were imaged by fluorescence and were observed to propagate from the laser focal point inside the cell. Photoinduced generation of Ca[sup 2+] waves can be performed at any point inside the cell, an improvement over previous mechanical or biochemical stimulation techniques. © 2001 American Institute of Physics.


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