TITLE

Dengue NS1 antigen detection: A useful tool in early diagnosis of dengue virus infection

AUTHOR(S)
Datta, S.; Wattal, C.
PUB. DATE
April 2010
SOURCE
Indian Journal of Medical Microbiology;Apr2010, Vol. 28 Issue 2, p107
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Purpose: This study was undertaken to evaluate the efficacy of NS1 antigen (Ag) assay as an early marker for dengue virus (DV) infection. Materials and Methods: Group I evaluated the performance of NS1 antigen (Ag) assay in comparison to MAC-ELISA and their detection rate when performed together in a single sample. Six hundred acute/early convalescent sera were screened by both the assays. Group II evaluated the efficacy of a single assay in 30 acute phase sera of paediatric OPD patients screened only by NS1 Ag assay. Group III evaluated the specificity of NS1 assay in comparison to MAC-ELISA on 40 samples included as controls. Results: In Group I, 140 (23.3%) and 235 (39.1%) samples were positive by NS1 assay and MAC-ELISA respectively. The detection rate increased to 320 (53.3%) when both the assays were used together on a single sample. NS1 Ag positivity varied from 71.42% to 28.4% in acute and early convalescent sera, conversely IgM detection rate was 93.61% and 6.38% in early convalescent and acute phase sera respectively (P < 0.0001). In Group II, 66.66% (20) samples were positive by NS1 assay. All the samples in Group III were negative showing 100% specificity of both the assays. Conclusion: NS1 Ag assay holds promise in early diagnosis of dengue infection. When used in combination with MAC-ELISA on a single sample it significantly improves the diagnostic algorithm without the requirement of paired sera.
ACCESSION #
50137876

 

Related Articles

  • Detection of human leptospirosis as a cause of acute fever by capture ELISA using a Leptospira interrogans serovar Copenhageni (M20) derived antigen. Canal, Enrique; Pollett, Simon; Heitzinger, Kristen; Gregory, Michael; Kasper, Matthew; Halsey, Eric; Meza, Yocelinda; Campos, Kalina; Perez, Juan; Meza, Rina; Bernal, Maruja; Guillen, Alfredo; Kochel, Tadeusz J.; Espinosa, Benjamin; Hall, Eric R.; Maves, Ryan C. // BMC Infectious Diseases;2013, Vol. 13 Issue 1, p1 

    Background: Leptospirosis is a potentially lethal zoonosis mainly affecting low-resource tropical countries, including Peru and its neighbouring countries. Timely diagnosis of leptospirosis is critical but may be challenging in the regions where it is most prevalent. The serodiagnostic gold...

  • Recombinant nucleocapsid-like particles from dengue-2 virus induce protective CD4+ and CD8+ cells against viral encephalitis in mice. Gil, Lázaro; López, Carlos; Lazo, Laura; Valdés, Iris; Marcos, Ernesto; Alonso, Ruby; Gambe, Ailyn; Martín, Jorge; Romero, Yaremis; Guzmán, María G.; Guillén, Gerardo; Hermida, Lisset // International Immunology;Oct2009, Vol. 21 Issue 10, p1175 

    Virus-like particles are a highly effective type of subunit vaccine that mimics the overall structure of virus particles without containing infectious genetic material. In this work, a particulate form of the recombinant capsid protein from dengue-2 was evaluated in mice to determine the level...

  • Vimentin is required for dengue virus serotype 2 infection but microtubules are not necessary for this process. Wei Chen; Na Gao; Jia-li Wang; Yan-ping Tian; Zong-tao Chen; Jing An // Archives of Virology;Sep2008, Vol. 153 Issue 9, p1777 

    The present study investigated the effect of microtubules (MTs) and vimentin during dengue virus serotype 2 (DV2) infection. Immunostaining showed that DV2 infection induced MT and vimentin reorganization. Colocalization of DV2 antigens with MTs or vimentin were often observed in ECV304 cells....

  • Diagnotic Efficacy of Saliva For Dengue - A Reality in Near Future? A Piloting Initiative. BANAVAR, SPOORTHI RAVI; VIDYA, G. S. // Journal of Clinical & Diagnostic Research;Mar2014, Vol. 8 Issue 3, p229 

    Background: Dengue, a mosquito-transmitted viral infection presents variable symptoms, including death. Due to their increasing incidences, early detection and improved diagnoses of severe cases are of prime importance. Currently, viral antigens and antibodies are detected by traditional...

  • Recombinant Envelope-Proteins with Mutations in the Conserved Fusion Loop Allow Specific Serological Diagnosis of Dengue-Infections. Rockstroh, Alexandra; Barzon, Luisa; Pacenti, Monia; Palù, Giorgio; Niedrig, Matthias; Ulbert, Sebastian // PLoS Neglected Tropical Diseases;11/13/2015, Vol. 9 Issue 10, p1 

    Dengue virus (DENV) is a mosquito-borne flavivirus and a major international public health concern in many tropical and sub-tropical areas worldwide. DENV is divided into four major serotypes, and infection with one serotype leads to immunity against the same, but not the other serotypes. The...

  • Characterization of Magnitude and Antigen Specificity of HLA-DP, DQ, and DRB3/4/5 Restricted DENV-Specific CD4+ T Cell Responses. Grifoni, Alba; Moore, Eugene; Voic, Hannah; Sidney, John; Phillips, Elizabeth; Jadi, Ramesh; Mallal, Simon; De Silva, Aruna D.; De Silva, Aravinda M.; Peters, Bjoern; Weiskopf, Daniela; Sette, Alessandro // Frontiers in Immunology;7/5/2019, p1 

    Background: Dengue Virus (DENV) associated disease is a major public health problem. Assessment of HLA class II restricted DENV-specific responses is relevant for immunopathology and definition of correlates of protection. While previous studies characterized responses restricted by the HLA-DRB1...

  • Anti-malignin antibody--a screening test for cancer? Friedlander, Ed // NCRHI Newsletter;Sep/Oct99, Vol. 22 Issue 5, p1 

    Informs on the anti-malignin antibody in serum (AMAS) blood test used for diagnosing cancer. Consideration of malignin as a tumor antigen; Credentials of the husband-and-wife team promoting the assay; Proponent's non-testing of the antibody on non-cancerous cells; Necessity of caution in...

  • Serum free light chain assay reduces the need for serum and urine immunofixation electrophoresis in the evaluation of monoclonal gammopathy. Fulton, Richard B.; Fernando, Suran L. // Annals of Clinical Biochemistry;Sep2009, Vol. 46 Issue 5, p407 

    Background: The potential for serum free light chain (sFLC) assay measurements to replace urine electrophoresis (uEPG) and to also diminish the need for serum immunofixation (sIFE) in the screening for monoclonal gammopathy was assessed. A testing algorithm for monoclonal protein was developed...

  • Assay may identify kidney cancer from blood samples. Overmyer, Mac // Urology Times;Nov98, Vol. 26 Issue 11, p8 

    Reports on researchers from Columbia Presbyterian Hospital's development of an assay that detects an antigen in peripheral blood that appears to be unique to renal cell carcinoma. Association of the antigen with tumor grade; Correlation in the histology of the primary tumor; Assay's value as a...

Share

Read the Article

Courtesy of THE LIBRARY OF VIRGINIA

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics