Molecular identification of a phytoplasma associated with Russian olive witches’ broom in Iran

Rashidi, Mahnaz; Ghosta, Youbert; Bahar, Masoud
June 2010
European Journal of Plant Pathology;Jun2010, Vol. 127 Issue 2, p157
Academic Journal
Russian olive trees ( Elaeagnus angustifolia) showing witches’ broom symptoms typical of phytoplasma infection were observed in the Urmia region of Iran. A phytoplasma named Russian olive witches’ broom phytoplasma (ROWBp-U) was detected from all symptomatic samples by amplification of the 16S rRNA gene and 16S/23S rDNA spacer region using the polymerase chain reaction (PCR) which gave a product of expected length. DNA from symptomless plants used as a negative control yielded no product. The sequence of the 16S rRNA gene and 16S/23S rDNA spacer region of ROWBp-U showed 99% similarity with the homologous genes of members of the aster yellows group. We also detected a phytoplasma in neighboring alfalfa plants (AlWBp-U) showing severe witches’ broom symptoms. An 1107 bp PCR product from the 16S rRNA gene showed 99% homology with the corresponding product in ROWBp-U, suggesting the presence of the same phytoplasma actively vectored in the area. Further observations showed that Russian olive trees with typical ROWB symptoms were present in an orchard near Tehran which is located over 530 km south-east of the original Urmia site. The corresponding sequence of this phytoplasma (ROWBp-T) showed 99% homology to that of the ROWBp-U. A sequence homology study based on the 16S rRNA gene and 16S/23S rDNA spacer region of ROWBp-U and other phytoplasmas showed that ROWBp-U is most closely related to the 16SrI group. To our knowledge, this is the first report of a phytoplasma infection in a member of the Elaeagnaceae.


Related Articles

  • Molecular analysis of ' Candidatus Phytoplasma aurantifolia' associated with phytoplasma diseases in Myanmar. Win, Nang; Jung, Hee-Young // Journal of General Plant Pathology;Jul2012, Vol. 78 Issue 4, p260 

    During 2010 and 2011, typical phytoplasma disease symptoms such as little leaves, phyllody and witches' brooms were observed on black gram, green gram, long bean, shaggy button weed and sesame plants from different regions of Myanmar. The symptomatic samples were analyzed by PCR using universal...

  • Characterization of phytoplasmas related to 'Candidatus Phytoplasma asteris' subgroup rpI-L in Iran. Vali-Sichani, Fereshteh; Bahar, Masoud; Zirak, Leila // Journal of Plant Protection Research;2014, Vol. 54 Issue 2, p199 

    In two of Iran's central provinces, several herbaceous plants showing phytoplasma disease symptoms were collected to detect 'Canididatus Phytoplasma asteris'-related phytoplasmas. Confirmation of an association of phytoplasmas with diseased plants was done using polymerase chain reaction (PCR)...

  • Rapid and reliable detection of phytoplasma by loop-mediated isothermal amplification targeting a housekeeping gene. Sugawara, Kyoko; Himeno, Misako; Keima, Takuya; Kitazawa, Yugo; Maejima, Kensaku; Oshima, Kenro; Namba, Shigetou // Journal of General Plant Pathology;Nov2012, Vol. 78 Issue 6, p389 

    Phytoplasmas are plant pathogenic bacteria that infect more than 700 plant species. Because phytoplasma-resistant cultivars are not available for the vast majority of crops, the most common practice to prevent phytoplasma diseases is to remove infected plants. Therefore, developing a rapid,...

  • ' Candidatus Phytoplasma aurantifolia'-related strain associated with tomato yellows disease in China. Dong, J.; Zhang, L.; Li, W.; McBeath, J.; Zhang, Z. // Journal of General Plant Pathology;Sep2013, Vol. 79 Issue 5, p366 

    A new disease of tomato plants with typical phytoplasma disease symptoms such as stunting, yellows, auxiliary shoot proliferation and phyllody was observed in Yunnan Province, southwest China in 2011. By a nested-PCR, phytoplasma were detected using the phytoplasma universal primers specific for...

  • Molecular identification of a phytoplasma associated with Ajwain ( Trachyspermum ammi) in India. Samad, A.; Panda, S.; Gupta, Mahesh; Ajayakumar, P.; Shukla, Ashutosh // European Journal of Plant Pathology;May2011, Vol. 130 Issue 1, p1 

    new disease of Trachyspermum ammi, commonly known as Ajwain in India, was observed in Lucknow, India. The symptoms included small chlorotic leaves, highly proliferating shoots, witches' broom appearance, shortened internodes and an overall stunted growth. Poor flower heads and fruit setting...

  • Duplex PCR for simultaneous detection of Begomovirus and Phytoplasma from naturally infected tomato. SWARNALATHA, P.; REDDY, M. KRISHNA // Pest Management In Horticultural Ecosystems;Jun2014, Vol. 20 Issue 1, p59 

    Symptoms associated with the tomato leaf curl disease (ToLCD) were observed in tomato fields from Southern Karnataka, India affected with the .big bud. phytoplasma. Surveys indicated incidence of tomato leaf curl disease from 27.5 to 74.3%, tomato big bud phytoplasma disease from 5.7 to 13.9%...

  • Validation of reference genes for RT-qPCR normalization in common bean during biotic and abiotic stresses. Borges, Aline; Tsai, Siu; Caldas, Danielle // Plant Cell Reports;May2012, Vol. 31 Issue 5, p827 

    Selection of reference genes is an essential consideration to increase the precision and quality of relative expression analysis by the quantitative RT-PCR method. The stability of eight expressed sequence tags was evaluated to define potential reference genes to study the differential...

  • Development of Real-Time PCR for in wood-detection of Ceratocystis platani, the agent of canker stain of Platanus spp. Pilotti, Massimo; Lumia, Valentina; Lernia, Giovanni; Brunetti, Angela // European Journal of Plant Pathology;Sep2012, Vol. 134 Issue 1, p61 

    Ceratocystis platani is a quarantine fungal pathogen agent of canker stain, a destructive disease affecting Platanus. Despite its diagnosis being critical for disease control, there is still no effective diagnostic tool as all known mycological and biological detection assays are problematical....

  • Enhanced Cloning Efficiency of Differential Display PCR Amplicons. Sanabria, Natasha M.; Dubery, Ian A. // Plant Molecular Biology Reporter;Sep2004, Vol. 22 Issue 3, p309 

    Differential display offers a rapid and simultaneous comparison of expression profiles of mRNA from variant cells having the same genetic background. An inherent flaw of the technique may yield false-positives on subsequent analyses. The only source of the band of interest is the denaturing...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics