TITLE

Validation of a two-dimensional gas chromatography mass spectrometry method for the simultaneous quantification of cannabidiol, ?9-tetrahydrocannabinol (THC), 11-hydroxy-THC, and 11-nor-9-carboxy-THC in plasma

AUTHOR(S)
Karschner, Erin; Barnes, Allan; Lowe, Ross; Scheidweiler, Karl; Huestis, Marilyn
PUB. DATE
May 2010
SOURCE
Analytical & Bioanalytical Chemistry;May2010, Vol. 397 Issue 2, p603
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
A sensitive analytical method for simultaneous quantification of sub-nanogram concentrations of cannabidiol (CBD), ?9-tetrahydrocannabinol (THC), 11-hydroxy-THC (11-OH-THC), and 11-nor-9-carboxy-THC (THCCOOH) in plasma is presented for monitoring cannabinoid pharmacotherapy and illicit cannabis use. Analytes were extracted from 1 mL plasma by solid-phase extraction, derivatized with N, O-bis(trimethylsilyl) trifluoroacetamide with 1% trimethylchlorosilane, and analyzed by two-dimensional gas chromatography mass spectrometry (2D-GCMS) with cryofocusing. The lower calibration curve was linear from 0.25�25 ng/mL for CBD and THC, 0.125-25 ng/mL for 11-OH-THC and 0.25-50 ng/mL for THCCOOH. A second higher linear range from 5�100 ng/mL, achieved through modification of injection parameters, was validated for THC, 11-OH-THC, and THCCOOH and was only implemented if concentrations exceeded the lower curve upper limit of linearity. This procedure prevented laborious re-extraction by allowing the same specimen to be re-injected for quantification on the high calibration curve. Intra- and inter-assay imprecision, determined at four quality control concentrations, were =7.8% CV. Analytical bias was within �9.2% of target and extraction efficiencies were =72.9% for all analytes. Analytes were stable when stored at 22�C for 16 h, 4�C for 48 h, after three freeze�thaw cycles at -20�C and when stored on the autosampler for 48 h. This sensitive and specific 2D-GCMS assay provides a new means of simultaneously quantifying CBD, THC and metabolite biomarkers in clinical medicine, forensic toxicology, workplace drug testing, and driving under the influence of drugs programs.
ACCESSION #
49444131

 

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