TITLE

Pyridinium aldoxime analysis by HPLC: the method for studies on pharmacokinetics and stability

AUTHOR(S)
Szegi, P.; Kal�sz, H.; Laufer, R.; Kuca, K.; Tekes, K.
PUB. DATE
May 2010
SOURCE
Analytical & Bioanalytical Chemistry;May2010, Vol. 397 Issue 2, p579
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Reversed-phase separation of various pyridinium aldoximes requires a certain concentration of ion-pairing agent, as their chemical structures contain two quaternary amines in the pyridinium ring. Adequate mobile phase is scouted on the basis of retention of pyridinium aldoxime (using the graph of k' versus concentration of an ion-pairing agent) compared to the chromatogram of the background peaks originated from the homogenate. Change in the ion-pairing agent concentration was more expressed for the elution of K-203 than that of the background peaks from the serum, brain and cerebrospinal fluid. Stability of K-203 was investigated using HPLC. Determination of K-203 in tissue samples requires homogenization using either trichloroacetic acid or perchloric acid. Fast degradation takes place at acidic pH. Adjusting pH to neutral in the possible shortest time frame helps to avoid degradation. Degradation of K-203 was easily followed by HPLC separation and monitoring the elution with an ultraviolet absorbance detector at 276 nm. Amperometric detection indicates only the decrease of K-203 content.
ACCESSION #
49444128

 

Related Articles

  • Monitoring the Pharmacokinetics of Pyridinium Aldoximes in the Body. Kalász, H.; Fűrész, J.; Tekes, K. // Mini Reviews in Medicinal Chemistry;May2009, Vol. 9 Issue 5, p596 

    A huge number of organophosphate poisonings occurring in agriculture, and a constant threat of misapplication of organophosphates as warfare agents require antidotes that efficiently improve the health-condition of intoxicated subjects. Pyridinium aldoximes are medically used to reactivate the...

  • Annas: Virtuous Person, Relativism, and the Circularity Objection. SARKAR, HUSAIN // Dialogue: Canadian Philosophical Review;Jun2015, Vol. 54 Issue 2, p285 

    This paper is informed by two principles: the Partiality Principle and the Impartiality Principle. Relying upon a relatively-unknown argument in Kant, the latter principle is stated and defended. The former principle is shown to be connected to Annas' claim, in her theory of virtue ethics, that...

  • SUBSTITUTED MONOQUATERNARY OXIMES AS REACTIVATORS OF CYCLOSARINAND CHLORPYRIFOS-INHIBITED ACETYLCHOLINESTERASE. Racakova, Veronika; Hrabinova, Martina; Jun, Daniel; Kuca, Kamil // Archives of Industrial Hygiene & Toxicology / Arhiv za Higijenu ;Dec2006, Vol. 57 Issue 4, p387 

    The article presents an in vitro study of three potential acetylcholinesterase (AChE) reactivators taken from a monoquaternary reactivator pralidoxime. The compounds used in the study include pyridinium-2-aldoxime-4- carbamoyl-N-methyl iodide, pyridinium-2-aldoxime-4-ethoxycarbonyl-N-methyl...

  • In vitro Evaluation of Aldoxime Interactions with Human Acetylcholinesterase. Kovarik, Zrinka; Čalic, Maja; Bosak, Anita; Šinko, Goran; Jelić, Dubravko // Croatica Chemica Acta;Apr2008, Vol. 81 Issue 1, p47 

    We related the ability of eleven pyridinium and imidazolium aldoximes to reactivate tabun-inhibited human erythrocyte acetylcholinesterase with their molecular properties. Using molecular mechanics we performed conformational analysis to determine the flexibility of the aldoximes. Semi-empirical...

  • Preparation of the Pyridinium Salts Differing in the Length of the N-Alkyl Substituent. Marek, Jan; Stodulka, Petr; Cabal, Jiri; Soukup, Ondrej; Pohanka, Miroslav; Korabecny, Jan; Musilek, Kamil; Kuca, Kamil // Molecules;Mar2010, Vol. 15 Issue 3, p1967 

    Quaternary pyridinium salts with chains ranging from C8 to C20 belong in the large group of cationic surfactants. In this paper, the preparation of such cationic surface active agents based on the pyridinium moiety and differing in the length of the N-alkyl chain is described. Additionally, HPLC...

  • Study on Medicinal Chemistry of K203 in Wistar Rats and Beagle Dogs. Kalasz, H.; Szegi, P.; Janoki, G.; Balogh, L.; Postenyi, Z.; Musilek, K.; Petroianu, G. A.; Siddiq, A.; Tekes, K. // Current Medicinal Chemistry;May2013, Vol. 20 Issue 14 - 16, p2137 

    K203 is an experimental bis–pyridinium mono–aldoxime type cholinesterase reactivator of potential use in organophosphate⁄organophosphonate poisoning. Pharmacokinetics of K203 were examined in Wistar rats and beagle dogsusing ion–pair HPLC. Serum and cerebrospinal fluid...

  • STRUCTURE-ACTIVITY APPROACH IN THE REACTIVATION OF TABUN-PHOSPHORYLATED HUMAN ACETYLCHOLINESTERASE WITH BISPYRIDINIUM para-ALDOXIMES / ODNOS STRUKTURE I AKTIVNOSTI U REAKTIVACIJI TABUNOM FOSFORILIRANE LJUDSKE ACETILKOLINESTERAZE BISPIRIDINIJEVIM para-ALDOKSIMIMA. Kovarik, Zrinka; Čalić, Maja; Šinko, Goran; Bosak, Anita // Archives of Industrial Hygiene & Toxicology / Arhiv za Higijenu ;Jun2007, Vol. 58 Issue 2, p201 

    The article focuses on a study that investigated the reactivation of tabun-phosphorylated human acetylcholinesterase with bispyridinium para-aldoximes under a structure-activity approach. It states that the flexibility of aldoxime as a feature for binding to the acetylcholinesterase active site...

  • Synthesis, Antidotal Effects and HPLC Behavior of Some Novel Pyridinium Aldoximes. Laufer, R.; Kalász, H.; Musilek, K.; Szegi, P.; Darvas, F.; Kuca, K.; Tekes, K. // Current Organic Chemistry;Mar2010, Vol. 14 Issue 5, p447 

    Pyridinium aldoximes are used as antidotes in organophosphate poisoning. In the reactivation process they form a complex with the organophosphates, even if the serine active site of acetylcholinesterase enzymes has been inactivated by the organophosphorus compounds. Essential disadvantage of the...

  • Cytotoxicity of Selected Pyridinium Oximes in Human SH-SY5Y Neuroblastoma Cell Line. Bognar, Svjetlana Kalanj; Foretić, Blaženka; Vukelić, Željka; Gulin, Tonko; Ježek, Davor // Croatica Chemica Acta;Apr2008, Vol. 81 Issue 1, p67 

    Pyridinium oximes are pharmacologically important nucleophylic agents acting as effective antidotes against poisoning by organophosphorus compounds that inhibit acetylcholinesterase (AChE; EC 3.1.1.7.). In this study, the cytotoxicity of 1-phenacylpyridinium-4-aldoxime chloride (FEPA-4) was...

Share

Read the Article

Courtesy of VIRGINIA BEACH PUBLIC LIBRARY AND SYSTEM

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics