TITLE

Chiral separation of (+)/(-)-catechin from sulfated and glucuronidated metabolites in human plasma after cocoa consumption

AUTHOR(S)
Ritter, Christina; Zimmermann, Benno; Galensa, Rudolf
PUB. DATE
May 2010
SOURCE
Analytical & Bioanalytical Chemistry;May2010, Vol. 397 Issue 2, p723
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Cocoa is well-known to be rich in flavan-3-ols. Previous analyses have established that alkaline treatment of cocoa beans results in epimerization of (-)-epicatechin to (-)-catechin and (+)-catechin to (+)-epicatechin. Now, the question is whether both epimers can be absorbed by the human organism. This paper describes sample preparation and an HPLC method for chiral determination of (+)/(-)-catechin from sulfated and glucuronidated metabolites in human plasma. The sample preparation includes enzymatic hydrolysis of the catechin metabolites, and solid-phase extraction (SPE). A PM-?-cyclodextrin column is used with a coulometric electrode-array detection (CEAD) system. The recovery of catechin ranges from 89.9 to 96.8%. The limit of detection is 5.9 ng mL-1 for (-)-catechin and 6.8 ng mL-1 for (+)-catechin, and the limit of quantification is 12.8 ng mL-1 for (-)-catechin and 16.9 ng mL-1 for (+)-catechin. The relative standard deviation of the method ranges from 0.9 to 1.5%. This method was successfully applied to human plasma after consumption of a cocoa drink. In one human self-experiment, (+)-catechin and (-)-catechin were found in human plasma, but metabolism of the two enantiomers differed. [Figure not available: see fulltext.]
ACCESSION #
49444111

 

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