Sp1 and KLF15 regulate basal transcription of the human LRP5 gene

Jiangxia Li; Yang Yang; Baichun Jiang; Xiyu Zhang; Yongxin Zou; Yaoqin Gong
January 2010
BMC Genetics;2010, Vol. 11, p12
Academic Journal
Background: LRP5, a member of the low density lipoprotein receptor superfamily, regulates diverse developmental processes in embryogenesis and maintains physiological homeostasis in adult organisms. However, how the expression of human LRP5 gene is regulated remains unclear. Results: In order to characterize the transcriptional regulation of human LRP5 gene, we cloned the 5' flanking region and evaluated its transcriptional activity in a luciferase reporter system. We demonstrated that both KLF15 and Sp1 binding sites between -72 bp and -53 bp contribute to the transcriptional activation of human LRP5 promoter. Chromatin immunoprecipitation assay demonstrated that the ubiquitous transcription factors KLF15 and Sp1 bind to this region. Using Drosophila SL2 cells, we showed that KLF15 and Sp1 trans-activated the LRP5 promoter in a manner dependent on the presence of Sp1-binding and KLF15-binding motifs. Conclusions: Both KLF15 and Sp1 binding sites contribute to the basal activity of human LRP5 promoter. This study provides the first insight into the mechanisms by which transcription of human LRP5 gene is regulated.


Related Articles

  • Wnt Secretion and Gradient Formation. Solis, Gonzalo P.; Lüchtenborg, Anne-Marie; Katanaev, Vladimir L. // International Journal of Molecular Sciences;Mar2013, Vol. 14 Issue 3, p5130 

    Concentration gradients formed by the lipid-modified morphogens of the Wnt family are known for their pivotal roles during embryogenesis and adult tissue homeostasis. Wnt morphogens are also implicated in a variety of human diseases, especially cancer. Therefore, the signaling cascades triggered...

  • Ectopically tethered CP190 induces large-scale chromatin decondensation. Ahanger, Sajad H.; Günther, Katharina; Weth, Oliver; Bartkuhn, Marek; Bhonde, Ramesh R.; Shouche, Yogesh S.; Renkawitz, Rainer // Scientific Reports;1/31/2014, p1 

    Insulator mediated alteration in higher-order chromatin and/or nucleosome organization is an important aspect of epigenetic gene regulation. Recent studies have suggested a key role for CP190 in such processes. In this study, we analysed the effects of ectopically tethered insulator factors on...

  • The JIL-1 Kinase Affects Telomere Expression in the Different Telomere Domains of Drosophila. Silva-Sousa, Rute; Casacuberta, Elena // PLoS ONE;Nov2013, Vol. 8 Issue 11, p1 

    In Drosophila, the non-LTR retrotransposons HeT-A, TART and TAHRE build a head-to-tail array of repetitions that constitute the telomere domain by targeted transposition at the end of the chromosome whenever needed. As a consequence, Drosophila telomeres have the peculiarity to harbor the genes...

  • Topoisomerase II plays a role in dosage compensation in Drosophila. Cugusi, Simona; Ramos, Edward; Ling, Huiping; Yokoyama, Ruth; Luk, Kevin M.; Lucchesi, John C. // Transcription (2154-1264);2013, Vol. 5, p1 

    In Drosophila, dosage compensation is mediated by the MSL complex, which binds numerous sites on the X chromosome in males and enhances the transcriptional rate of a substantial number of X-linked genes. We have determined that topoisomerase II (Topo II) is enriched on dosage compensated genes,...

  • An in vivo RNA interference screen identifies gene networks controlling Drosophila melanogaster blood cell homeostasis. Avet-Rochex, Amélie; Boyer, Karène; Polesello, Cédric; Gobert, Vanessa; Osman, Dani; Roch, Fernando; Augé, Benoit; Zanet, Jennifer; Haenlin, Marc; Waltzer, Lucas // BMC Developmental Biology;2010, Vol. 10, p65 

    Background: In metazoans, the hematopoietic system plays a key role both in normal development and in defense of the organism. In Drosophila, the cellular immune response involves three types of blood cells: plasmatocytes, crystal cells and lamellocytes. This last cell type is barely present in...

  • The Cloning and Characterization of the Histone Acetyltransferase Human Homolog Dmel\TIP60 in Drosophila melanogaster: Dmel\TIP60 Is Essential for Multicellular Development. Xianmin Zhu; Neetu Singh; Donnelly, Christopher; Boimel, Pamela; Elefant, Felice // Genetics;Mar2007, Vol. 175 Issue 3, p1229 

    Chromatin packaging directly influences gene programming as it permits only certain portions of the genome to be activated in any given developmental stage, cell, and tissue type. Histone acetyltransferases (HATs) are a key class of chromatin regulatory proteins that mediate such developmental...

  • Evidence of Spatially Varying Selection Acting on Four Chromatin-Remodeling Loci in Drosophila melanogaster. Levine, Mia T.; Begun, David J. // Genetics;May2008, Vol. 179 Issue 1, p475 

    The packaging of DNA into proper chromatin structure contributes to transcriptional regulation. This packaging is environment sensitive, yet its role in adaptation to novel environmental conditions is completely unknown. We set out to identify candidate chromatin-remodeling loci that are...

  • Tissue-specific analysis of chromatin state identifies temporal signatures of enhancer activity during embryonic development. Bonn, Stefan; Zinzen, Robert P; Girardot, Charles; Gustafson, E Hilary; Perez-Gonzalez, Alexis; Delhomme, Nicolas; Ghavi-Helm, Yad; Wilczy?ski, Bartek; Riddell, Andrew; Furlong, Eileen E M // Nature Genetics;Feb2012, Vol. 44 Issue 2, p148 

    Chromatin modifications are associated with many aspects of gene expression, yet their role in cellular transitions during development remains elusive. Here, we use a new approach to obtain cell type-specific information on chromatin state and RNA polymerase II (Pol II) occupancy within the...

  • Calcium Spikes in Epithelium: study on Drosophila early embryos. Markova, Olga; Sénatore, Sébastien; Chardès, Claire; Lenne, Pierre-François // Scientific Reports;7/24/2015, p11379 

    Calcium ion acts in nearly every aspect of cellular life. The versatility and specificity required for such a ubiquitous role is ensured by the spatio-temporal dynamics of calcium concentration variations. While calcium signal dynamics has been extensively studied in cell cultures and adult...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics