Monoclonal antibodies: production and use

McGregor, A.M.
October 1981
British Medical Journal (Clinical Research Edition);10/31/1981, Vol. 283 Issue 6300, p1143
Academic Journal
Examines the production and use of monoclonal antibodies in the purification of substances in diagnosis and treatment. Costs of production of antibodies; Validity of products of human hybridomas for clinical use; Importance of technological development on the production of antibodies.


Related Articles

  • Metabolic flux-based modeling of mAb production during batch and fed-batch operations. Penny Dorka; Christian Fischer; Hector Budman; Jeno Scharer // Bioprocess & Biosystems Engineering;Feb2009, Vol. 32 Issue 2, p183 

    Abstract  This paper proposes mathematical models that predict the physiology, growth behavior and productivity of hybridoma cells in both batch and fed-batch systems. Murine hybridoma 130-8F producing anti-F-glycoprotein monoclonal antibody was employed as a model system. A systematic...

  • Heterohybridoma for the production of non murine monoclonal antibodies. Chanu, Kh.Victoria; Ali, M. Ayub // Veterinary World;Aug2010, Vol. 3 Issue 8, p390 

    Hybridoma technology described by kohler and Milstein produce only mouse immunoglobulins. Such immunoglobulins have limited use due to its negative side effects such as the recipient's immune response. The production of a non murine monoclonal antibody to combat the problems of murine monoclonal...

  • Industrial processes with animal cells. Kretzmer, G. // Applied Microbiology & Biotechnology;Jul2002, Vol. 59 Issue 2/3, p135 

    Industrial processes involving animal cells for the production of useful products still seem to be rather uncommon. Nevertheless, during the last four decades of the last century the number of relevant processes has increased from production of virus vaccines to monoclonal antibodies and finally...

  • An efficient system to generate monoclonal antibodies against membrane-associated proteins by immunisation with antigen-expressing mammalian cells. Dreyer, Anita M.; Beauchamp, Jeremy; Matile, Hugues; Pluschke, Gerd // BMC Biotechnology;2010, Vol. 10, p87 

    Background: The generation of monoclonal antibodies specific for protein antigens usually depends on purified recombinant protein for both immunisation and hybridoma screening. Purification of recombinant protein in sufficient yield and purity is a tedious undertaking and can be demanding...

  • Products of New Biotechnologies Approved by the FDA.  // Clinical Pediatrics;Apr1987, Vol. 26 Issue 4, p188 

    This article presents information related to several products manufactured by means of new biotechnology that were approved in 1986. Some of these represent major therapeutic advances while others offer important alternatives to traditional therapies. It is anticipated that there will be...

  • Generation and characterization of a scFv against recombinant coat protein of the geminivirus tomato leaf curl New Delhi virus. Zakri, Adel M.; Ziegler, Angelika; Torrance, Lesley; Fischer, Rainer; Commandeur, Ulrich // Archives of Virology;Mar2010, Vol. 155 Issue 3, p335 

    We report the establishment of a hybridoma cell line secreting the monoclonal antibody (mAb) HAV, which recognizes the coat (AV1) protein of tomato leaf curl New Delhi virus (ToLCNDV), a begomovirus. The cell line was obtained following immunization of mice with purified recombinant AV1 fused to...

  • Primary Structures and Chain Dominance of Anti-DNA Antibodies. Jeong Soo Park; Young-Tai Kim; Hee Yong Chung; Kwanghee Baek; Young-Ju Jang // Molecules & Cells (Springer Science & Business Media B.V.);2001, Vol. 11 Issue 1, p55 

    Using several anti-DNA autoantibodies, we analyzed the relative involvement of heavy and light chains in their interactions with DNA. We previously obtained eight hybridomas producing monoclonal anti-DMA autoantibodies by fusing spleen cells from an MRL-lpr/lpr mouse with myeloma cells. The...

  • Production and evaluation of monoclonal antibodies for alphafetoprotein. Abdel-Ghany, I. Y.; El-Bayoumy, A. S.; Elmouhty, N. R. A.; Shafik, H. M. // Journal of Radioanalytical & Nuclear Chemistry;Jun2009, Vol. 280 Issue 3, p651 

    Human AFP was used as an antigen for the development of monoclonal antibodies by the hybridoma technique. Balb/c mice were immunized with highly purified AFP. The preparation of I125-AFP was carried out by lactoperoxidase oxidation method, preparation of AFP standards was carried out from cord...

  • © COMPETITIVE RADIOIMMUNOASSAYS USING HYBRIDOMA AND ANTI-IDIOTYPE ANTIBODIES IN IDENTIFICATION OF ANTIBODY RESPONSES TO, AND ANTIGENS OF, SCHISTOSOMA JAPONICUM. Mitchell, Graham F.; Garcia, Edito G.; Cruise, Kathy M. // Australian Journal of Experimental Biology & Medical Science;Feb1983, Vol. 61 Issue 1, p27 

    A hybridoma-derived monoclonal antibody, L134, with apparent specificity for Schistosoma japonicum adult worms has immunodiagnostic potential for schistosomiasis japonica in the Philippines. Sera from known infected individuals will inhibit the binding of 125-l-labeIled I.134 to a crude adult...


Read the Article

Courtesy of

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics