Single-molecule analysis: A ribosome in action

Brakmann, Susanne
April 2010
Nature;4/15/2010, Vol. 464 Issue 7291, p987
Academic Journal
The article focuses on a study which reports the use of a sensitive single-molecule detection technique to observe a complex interplay of various cellular components into which protein synthesis is involved. The approach of the study is based on a set of tRNAs labelled with distinct fluorophores allowing their immediate determination after ribosomal binding. The translation and the physiologically pertinent concentrations of tRNA are examined in the study along with factors that use small reaction vessels known as zero-mode waveguides.


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