TITLE

Development and Validation of Second-Derivative Spectrophotometry Method for Simultaneous Estimation of Alprazolam and Fluoxetine Hydrochloride in Pure Powder and Tablet Formulation and Its Comparison with HPLC Method

AUTHOR(S)
Patel, Rashmin B.; Patel, Mrunali R.; Shankar, Madhira B.; Bhatt, Kashyap K.
PUB. DATE
March 2009
SOURCE
Eurasian Journal of Analytical Chemistry;2009, Vol. 4 Issue 1, p76
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
This paper describes validated Second Derivative Spectrophotometry (D2) method for simultaneous estimation of Alprazolam (ALP) and Fluoxetine hydrochloride (FXT) in pure powder and formulation. D2 method, applying the peak zero method, was developed for the determination of ALP and FXT in their combined tablet formulations without prior separation. The solutions of standard and sample were prepared in 0.1M HCl. Quantitative determination of the drugs was performed at 232.14 nm and at 225.25 nm (N = Δ4; γ = 2.8) for ALP and FXT, respectively. Proposed D2 method was evaluated for the different validation parameters. The specificity test showed that there was no interference from excipients commonly found in the commercial pharmaceutical formulations at analytical wavelength of ALP and FXT. Quantification was achieved over the concentration range 4-14 µg.mL-1 for both drugs with mean recovery of 99.36 ± 0.84 and 99.60 ± 0.93 % for ALP and FXT, respectively. This method is simple, precise, and sensitive and applicable for the simultaneous determination of ALP and FXT in pure powder and formulation. The method was compared to high-performance liquid chromatography (HPLC) method, which was reported for the same drugs. No significant difference was found between the methods for ALP and FXT quantitatio
ACCESSION #
48948928

 

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