Air-coupled broadband ultrasonic spectroscopy as a new non-invasive and non-contact method for the determination of leaf water status

Sancho-Knapik, Domingo; Álvarez-Arenas, Tom´s Gómez; Peguero-Pina, José Javier; Gil-Pelegrín, Eustaquio
April 2010
Journal of Experimental Botany;Apr2010, Vol. 61 Issue 5, p1385
Academic Journal
The implementation of non-destructive methods for the study of water changes within plant tissues and/or organs has been a target for some time in plant physiology. Recent advances in air-coupled ultrasonic spectroscopy have enabled ultrasonic waves to be applied to the on-line and real-time assessment of the water content of different materials. In this study, this technique has been applied as a non-destructive, non-invasive, non-contact, and repeatable method for the determination of water status in Populus×euramericana and Prunus laurocerasus leaves. Frequency spectra of the transmittance of ultrasounds through plant leaves reveal the presence of at least one resonance. At this resonant frequency, transmittance is at its maximum. This work demonstrates that changes in leaf relative water content (RWC) and water potential (Ψ) for both species can be accurately monitored by the corresponding changes in resonant frequency. The differential response found between both species may be due to the contrasting leaf structural features and the differences found in the parameters derived from the P–V curves. The turgor loss point has been precisely defined by this new technique, as it is derived from the lack of significant differences between the relative water content at the turgor loss point (RWCTLP) obtained from P–V curves and ultrasonic measurements. The measurement of the turgor gradient between two different points of a naturally transpiring leaf is easily carried out with the method introduced here. Therefore, such a procedure can be an accurate tool for the study of all processes where changes in leaf water status are involved.


Related Articles

  • Leaf-shape variation of Paederia foetida in Japan: reexamination of the small, narrow leaf form from Miyajima Island. Tsukaya, Hirokazu; Imaichi, Ryoko; Yokoyama, Jun // Journal of Plant Research;Jul2006, Vol. 119 Issue 4, p303 

    Variations in Paederia foetida L. leaf shape were examined to evaluate the taxonomic validity of the small, narrow leaf form of P. foetida f. microphylla Honda from Miyajima Island, Honshu, Japan. There is considerable variation in P. foetida individuals in terms of leaf size and leaf index...

  • Development and typification of gynoecium in representatives of subfamily Silenoideae (Caryophyllaceae). Kotelnikova, N. S.; Shamrov, I. I. // Vestnik Sankt-Peterburgskogo universiteta, Seriia 7: Geologia, G;2012, Issue 4, p136 

    The article studies the gynoecium morphogenesis features of four species of Silenoideae subfamily of the family Caryophyllaceae, members of the tribe Lychnideae (Viscaria vulgaris, Coccyganthe flos-cuculi) and Diantheae (Saponaria officinalis, Dianthus arenarius). The study of gynoecium in...

  • B chromosome:. Schlegel, Rolf H. J. // Encyclopedic Dictionary of Plant Breeding & Related Subjects;2003, p47 

    A definition of the term "B chromosome" is presented. It refers to any chromosome of a heterogeneous group of chromosomes present in several plant species. They differ in their morphology, numerical variation, meiotic pairing, and mitotic behavior

  • Genome-Dependent Factors in the Development of Leaf Phototrophic Tissues in Diploid and Alloploid Wheat Species. Khramtsova, E. V.; Kiseleva, I. S. // Russian Journal of Plant Physiology;Mar/Apr2004, Vol. 51 Issue 2, p249 

    Growth and mesostructure of the photosynthetic apparatus were studied in leaves of ten Triticum L. species. Plants with the Au genome were shown to develop larger leaf assimilation areas due to expanding areas of individual leaves and an increase in the absolute growth rate. Leaf and mesophyll...

  • The influence of epidermal windows on the light environment within the leaves of six succulents. Egbert, Kathryn J.; Martin, Craig E.; Vogelmann, Thomas C. // Journal of Experimental Botany;May2008, Vol. 59 Issue 7, p1863 

    An omni-directional fibre optic microprobe was used to measure the quantity and quality of light within the leaves of six succulents having epidermal windows, three species having a subterranean growth habit (Haworthia truncata, Lithops olivacea, and Opthalmophyllum longum) and three growing...

  • A new species of spiny Solanum (Solanaceae) from Peru. Stern, Stephen R. // PhytoKeys;2014, Issue 39, p27 

    A new species of Solanum is described from Peru. Solanum junctum S. Stern & M. Nee, sp nov. is a member of the Torva clade of the spiny solanums (Leptostemonum clade). The narrow corolla lobes and recurved prickles of S. junctum are similar to species in the Micracantha clade, but S. junctum...

  • Resurrection Plants and the Secrets of Eternal Leaf. SCOTT, PETER // Annals of Botany;Vol. 85 Issue 2, p159 

    Most higher plants possess a phase in their life cycle in which tissues can survive desiccation. However, this is restricted to specialized tissues such as seeds and pollen. Resurrection plants are remarkable in that they can tolerate almost complete water loss in their vegetative tissues. The...

  • Comparative Studies of Mucilage Cells in Different Organs in Some Species of Malva, Althaea and Alcea. Pakravan, M.; Abedinzadeh, H.; Safaeepur, J. // Pakistan Journal of Biological Sciences;2007, Vol. 10 Issue 15, p2603 

    Distribution of Mucilage Cells (MC) in leaves and petals of two species of Malva L. :Malva neglecta Wallr and M. nicaeensis All, one species of Altheae L.: A. officinalis L. and one species of Alcea L: A. angulata (Freyn and Sint.) Freyn and Sint. ex Iljin, have studied. Except of A. angulata...

  • CARACTERES XEROMORFOS FOLIARES DE NASSAUVIA LAGASCAE (COMPOSITAE). Ragonese, Ana Maria // Darwiniana;1990, Vol. 30 Issue 1-4, p1 

    The xeromorphic leaf characters of Nassauvia lagascae (Don) Meigen and its varieties, N. lagascae var. lanata (Phil.) Skottsberg and N. lagascae var. globosa Skottsberg, referred to section Caloptilium of subgenus Nassauvia, are described. All are herbaceous perennial plants, forming loose...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics