TITLE

Isolation of N-linked glycopeptides by hydrazine-functionalized magnetic particles

AUTHOR(S)
Shisheng Sun; Ganglong Yang; Ting Wang; Qinzhe Wang; Chao Chen; Zheng Li
PUB. DATE
April 2010
SOURCE
Analytical & Bioanalytical Chemistry;Apr2010, Vol. 396 Issue 8, p3071
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
We introduce a novel combination of magnetic particles with hydrazine chemistry, dubbed as hydrazine-functionalized magnetic particles (HFMP) for isolation of glycopeptides. Four methods have been developed and compared for the production of HFMP by hydrazine modification of the surface of the carboxyl and epoxy-silanized magnetic particles, respectively. The evaluation of the capability and specificity of HFMP as well as the optimization of the coupling condition for capturing of glycoproteins were systematically investigated. The results showed that HFMP prepared by adipic dihydrazide functionalization from carboxyl-silanized magnetic particles (HFCA) displayed the maximum capture capacity and isolated efficiency for glycoprotein. When measured with glycoproteins, the capacity of the HFCA (1 g) for coupling bovine fetuin was 130 ± 5.3 mg. The capability of this method was also confirmed by successful isolation of all formerly glycosylated peptides from standard glycoproteins and identification of their glycosylation sites, which demonstrated the feasibility of the HFCA as an alternative solid support for isolation of glycoproteins/glycopeptides. [Figure not available: see fulltext.]
ACCESSION #
48746688

 

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