TITLE

Use of liquid chromatography coupled to low- and high-resolution linear ion trap mass spectrometry for studying the metabolism of paynantheine, an alkaloid of the herbal drug Kratom in rat and human urine

AUTHOR(S)
Philipp, Anika A.; Wissenbach, Dirk K.; Weber, Armin. A.; Zapp, Josef; Zoerntlein, Siegfried W.; Kanogsunthornrat, Jidapha; Maurer, Hans H.
PUB. DATE
April 2010
SOURCE
Analytical & Bioanalytical Chemistry;Apr2010, Vol. 396 Issue 7, p2379
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
The Thai medicinal plant Mitragyna speciosa ( Kratom in Thai) is misused as a herbal drug of abuse. During studies on the main Kratom alkaloid mitragynine (MG) in rats and humans, several dehydro analogs could be detected in urine of Kratom users, which were not found in rat urine after administration of pure MG. Questions arose as to whether these compounds are formed from MG only by humans or whether they are metabolites formed from the second abundant Kratom alkaloid paynantheine (PAY), the dehydro analog of MG. Therefore, the aim of the presented study was to identify the phase I and II metabolites of PAY in rat urine after administration of the pure alkaloid. This was first isolated from Kratom leaves. Liquid chromatography–linear ion trap mass spectrometry provided detailed structure information of the metabolites in the MSn mode particularly with high resolution. Besides PAY, the following phase I metabolites could be identified: 9- O-demethyl PAY, 16-carboxy PAY, 9- O-demethyl-16-carboxy PAY, 17- O-demethyl PAY, 17- O-demethyl-16,17-dihydro PAY, 9,17- O-bisdemethyl PAY, 9,17- O-bisdemethyl-16,17-dihydro PAY, 17-carboxy-16,17-dihydro PAY, and 9- O-demethyl-17-carboxy-16,17-dihydro PAY. These metabolites indicated that PAY was metabolized via the same pathways as MG. Several metabolites were excreted as glucuronides or sulfates. The metabolism studies in rats showed that PAY and its metabolites corresponded to the MG-related dehydro compounds detected in urine of the Kratom users. In conclusion, PAY and its metabolites may be further markers for a Kratom abuse in addition of MG and its metabolites. [Figure not available: see fulltext.]
ACCESSION #
48645721

 

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