TITLE

Simultaneous quantitative determination of α-ketoglutaric acid and 5-hydroxymethylfurfural in human plasma by gas chromatography–mass spectrometry

AUTHOR(S)
Wagner, Bernhard M.; Donnarumma, Fabrizio; Wintersteiger, Reinhold; Windischhofer, Werner; Leis, Hans J.
PUB. DATE
April 2010
SOURCE
Analytical & Bioanalytical Chemistry;Apr2010, Vol. 396 Issue 7, p2629
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
α-Ketoglutaric acid (α-KG) and 5-hydroxymethylfurfural (5-HMF) are currently under investigation as promising cancer cell damaging agents. A method for the simultaneous quantitative determination of α-KG and 5-HMF in human plasma was established for screening these compounds in human plasma. Plasma samples were directly treated with O-(2,3,4,5,6-pentafluorobenzyl) hydroxylamine hydrochloride to form the corresponding oximes, thus facilitating subsequent liquid–liquid extraction. After formation of the trimethylsilyl ethers, samples were analyzed by gas chromatography with electron ionization mass spectrometry. Stable isotope labeled standards were used, the preparation of 13C6-5-HMF is described. Limits of quantitation were set to 0.938 µg/mL for α-KG and 0.156 µg/mL for 5-HMF. Inter-day accuracy was ≤93.7% (α-KG) and ≤92.8% (5-HMF). Inter-day precision was ≤6.0% (α-KG) and ≤4.6% (5-HMF). The method has been successfully applied to pharmacokinetic profiling of the compounds after intravenous application.
ACCESSION #
48645689

 

Related Articles

  • GC-MS Analysis of Azima tetracantha (LAM). Gayathri, G.; Nair, Bindu R.; Babu, V. // Journal of Pharmacy Research;2012, Vol. 5 Issue 7, p3746 

    In this study, the bioactive compounds in the methanolic leaf extract of Azima tetracantha was evaluated using Gas Chromatography-Mass Spectroscopy. The study revealed the presence of the major compound, Friedo-b':a'-neogammacer-5-en-3-one (23.36%) along with other 15 compounds.

  • A Novel Temperature-Controlled Ionic Liquid Dispersive Liquid Phase Microextraction for Determination of Dicofol and DDT in Environmental Water Samples Prior to Gas Chromatography Mass Spectrometry. Li Zaijun; Chen Peipei; Yu Cuiping; Fang Yinjun; Wang Zhongyun; Li Ming; Shan Haixia // Current Analytical Chemistry;Oct2009, Vol. 5 Issue 4, p324 

    A novel dispersive liquid phase microextraction using a new temperature-controlled ionic liquid 1-isooctyl-3-methylimidazolium hexafluorophosphate was proposed as novel medium for the determination of dicofol and DDT in environmental water. For one new extraction, a 1.0 ml methanol solution...

  • Glomerin and homoglomerin from the North American pill millipede Onomeris sinuata (Loomis, 1943) (Diplopoda, Pentazonia, Glomeridae). Shear, William A.; Jones, Tappey H.; Wesener, Thomas // International Journal of Myriapodology (Pensoft Publishers);Apr2011, Vol. 4, p1 

    Specimens of the North American glomerid millipede Onomeris sinuata (Loomis, 1943) were collected at the type locality in Alabama, USA, and maintained briefly in the laboratory in native leaf litter. The millipedes could not be induced to produce defensive secretions by rough handling, tapping,...

  • Cytotoxic effect of Alpinia scabra (Blume) Náves extracts on human breast and ovarian cancer cells. Subba Reddy, Annushuya; Nurestri Abd Malek, Sri; Ibrahim, Halijah; Kae Shin Sim // BMC Complementary & Alternative Medicine;2013, Vol. 13 Issue 1, p314 

    Background: Alpinia scabra, locally known as 'Lengkuas raya', is an aromatic, perennial and rhizomatous herb from the family Zingiberaceae. It is a wild species which grows largely on mountains at moderate elevations in Peninsular Malaysia, but it can also survive in the lowlands like in the...

  • Effect of Citronella Oil on Time Kill Profile, Leakage and Morphological Changes of Propionibacterium acnes. Lertsatitthanakorn, Pilanthana; Taweechaisupapong, Suwimol; Arunyanart, Channarong; Aromdee, Chantana; Khunkitti, Watcharee // Journal of Essential Oil Research;May/Jun2010, Vol. 22 Issue 3, p270 

    The aim of this study was to investigate the effect of citronella oil (Java type) (Cymbopogon winterianus Jowitt) on Propionibacterium acnes DMST 14916. Citronella oil compositions were determined by gas chromatography mass spectrometry (GC/MS). Anti-P. acnes activity of citronella oil and its...

  • Antiproliferative activity of hexane extract from Tunisian Cistus libanotis, Cistus monspeliensis and Cistus villosus. Jemia, Mariem Ben; Kchouk, Mohamed Elyes; Senatore, Felice; Autore, Giuseppina; Marzocco, Stefania; Feo, Vincenzo De; Bruno, Maurizio // Chemistry Central Journal;2013, Vol. 7 Issue 1, p1 

    Background: As a part of our investigation on Tunisian medicinal plants, we have carried out a phytochemical investigation of the hexane extracts from leaves of Cistus libanotis, C. villosus and C. monspeliensis, evualuating also their possible antiproliferative activity in vitro. Results: The...

  • Cell line dependence of metabolite leakage in metabolome analyses of adherent normal and cancer cell lines. Kapoore, Rahul; Coyle, Rachael; Staton, Carolyn; Brown, Nicola; Vaidyanathan, Seetharaman // Metabolomics;Dec2015, Vol. 11 Issue 6, p1743 

    Analysis of the metabolome can be sample and cell dependent. In this study, we compared two conventional pre-treatment approaches (trypsinization and cell scraping) in three adherently grown mammalian cell lines (two breast cancer cell lines MDA-MB-436, MCF-7 and an endothelial cell...

  • Preliminary evaluation of production and characterization of wood vinegar from rubberwood. Ratanapisit, Juraivan; Apiraksakul, Sunanta; Rerngnarong, Athirat; Chungsiriporn, Juntima; Bunyakarn, Charun // Songklanakarin Journal of Science & Technology;2009, Vol. 31 Issue 3, p343 

    This study deals with a slow pyrolysis of rubberwood in a furnace at an atmospheric pressure to produce pyroligneous liquid or wood vinegar. Pyroligneous liquids produced were separated into two fractions based on temperatures in a furnace. The yields of wood vinegar were quantified. Also pH, SG...

  • Antistaphylococcal activity of Inula helenium L. root essential oil: eudesmane sesquiterpene lactones induce cell membrane damage. Stojanović-Radić, Z.; Čomić, Lj.; Radulović, N.; Blagojević, P.; Denić, M.; Miltojević, A.; Rajković, J.; Mihajilov-Krstev, T. // European Journal of Clinical Microbiology & Infectious Diseases;Jun2012, Vol. 31 Issue 6, p1015 

    The purpose of this study was to investigate the inhibitory/bactericidal activity and cell membrane effects of the hydrodistilled essential oil of Inula helenium L. roots against Staphylococcus aureus. Additionally, detailed chemical investigation was done in order to pinpoint the most active...

Share

Read the Article

Courtesy of THE LIBRARY OF VIRGINIA

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics