TITLE

Butyrate-Induced Cell Death and Differentiation Are Associated with Distinct Patterns of ROS in HT29-Derived Human Colon Cancer Cells

AUTHOR(S)
Domokos, Monika; Jakus, Judit; Szeker, Krisztina; Csizinszky, Rita; Csiko, György; Neogrady, Zsuzsanna; Csordas, Adam; Galfi, Peter
PUB. DATE
April 2010
SOURCE
Digestive Diseases & Sciences;Apr2010, Vol. 55 Issue 4, p920
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
To investigate the role of reactive oxygen species (ROS) induced by butyrate in tumor cells, we compared HT29R, an HT29-derived human colon cancer cell line refractory to butyrate-induced cell differentiation but highly sensitive to cell death, with the differentiation-positive HT29-12 and HT29-21 cell lines (exhibiting low sensitivity to butyrate-induced cell death), with respect to levels of butyrate-induced free radicals (FRs), ROS, and H2O2. Dose-dependent increase of FRs (as determined by electron spin resonance spectroscopy) and ROS (dichlorofluorescein assay) was induced in HT29R, but not in HT29-12 and HT29-21 cells, where, in contrast to HT29R, a dose-dependent increase of H2O2 release (phenol red assay) was induced by butyrate. The mode of butyrate-induced cell death in HT29R cells was of a mixed type with necrosis predominating, which, however, switched to apoptosis as the major type of cell death in the presence of the drugs 1,5-dihydroxyisoquinoline, resveratrol, or cyclosporine A. The results suggest that FRs and ROS induced by butyrate in HT29R cells are products of cell death, while H2O2 induced in HT29-12 and HT29-21 cells is functionally related to cell differentiation.
ACCESSION #
48600633

 

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