TITLE

On-column derivatization with o-phthaldialdehyde for fast determination of homocysteine in human urine

AUTHOR(S)
Głowacki, Rafał; Borowczyk, Kamila; Bald, Edward; Jakubowski, Hieronim
PUB. DATE
March 2010
SOURCE
Analytical & Bioanalytical Chemistry;Mar2010, Vol. 396 Issue 6, p2363
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
A new assay for urinary homocysteine is described. The assay relies on an on-column derivatization with o-phthaldialdehyde, using a reversed-phase HPLC column and detection/quantification by fluorescence. The analysis time for reduced and total homocysteine, including sample work-up, was 5 and 13 min, respectively. Quantification limit was 25 nM.
ACCESSION #
48536696

 

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