See what you eat—broad GMO screening with microarrays

Von Götz, Franz
March 2010
Analytical & Bioanalytical Chemistry;Mar2010, Vol. 396 Issue 6, p1961
Academic Journal
Despite the controversy of whether genetically modified organisms (GMOs) are beneficial or harmful for humans, animals, and/or ecosystems, the number of cultivated GMOs is increasing every year. Many countries and federations have implemented safety and surveillance systems for GMOs. Potent testing technologies need to be developed and implemented to monitor the increasing number of GMOs. First, these GMO tests need to be comprehensive, i.e., should detect all, or at least the most important, GMOs on the market. This type of GMO screening requires a high degree of parallel tests or multiplexing. To date, DNA microarrays have the highest number of multiplexing capabilities when nucleic acids are analyzed. This trend article focuses on the evolution of DNA microarrays for GMO testing. Over the last 7 years, combinations of multiplex PCR detection and microarray detection have been developed to qualitatively assess the presence of GMOs. One example is the commercially available DualChip® GMO (Eppendorf, Germany; ), which is the only GMO screening system successfully validated in a multicenter study. With use of innovative amplification techniques, promising steps have recently been taken to make GMO detection with microarrays quantitative. [Figure not available: see fulltext.]


Related Articles

  • Validating the Vysis Genosensor 300 Microarray Platform for Use in a Diagnostic Laboratory.  // Journal of Medical Genetics;Sep2004 Supplement, Vol. 41, pS58 

    Presents an abstract of the study entitled "Validating the Vysis Genosensor 300 Microarray Platform for Use in a Diagnostic Laboratory," by Samantha Bradley, D. J. McMullan, D. E. C. Baker, E. V. Davison and M. J. Griffiths, presented during the British Human Genetics Conference held at the...

  • A PCR-microarray method for the screening of genetically modified organisms. Hamels, Sandrine; Glouden, Thomas; Gillard, Karine; Mazzara, Marco; Debode, Frédéric; Foti, Nicoletta; Sneyers, Myriam; Esteve Nuez, Teresa; Pla, Maria; Berben, Gilbert; Moens, William; Bertheau, Yves; Audéon, Colette; Van den Eede, Guy; Remacle, José // European Food Research & Technology;Feb2009, Vol. 228 Issue 4, p531 

    A new method to screen and to identify genetically modified organisms (GMO) is presented in this paper. It is based on the detection of multiple genetic elements common to GMO by their amplification via PCR followed by direct hybridisation of the amplicons on microarray. The pattern of the...

  • Non-GM feed becoming scarce. Casey, Scott // Poultry World;Nov2010, Vol. 164 Issue 11, p8 

    The article reports on the warning issued by feed manufacturers in Europe regarding the difficulty in securing genetically modified (GM)-free feed supplies.

  • How to use... microarray comparative genomic hybridisation to investigate developmental disorders. Kharbanda, Mira; Tolmie, John; Joss, Shelagh // Archives of Disease in Childhood -- Education & Practice Edition;Feb2015, Vol. 100 Issue 1, p24 

    Array-comparative genomic hybridisation (array-CGH) is a relatively new test that permits close scrutiny of chromosomal structure to detect genomic microdeletions and microduplications that are invisible in a conventional karyotype. Array-CGH is now the 'first-line' genetic test in the...

  • Application of next-generation sequencing technology for comprehensive aneuploidy screening of blastocysts in clinical preimplantation genetic screening cycles. Fiorentino, Francesco; Bono, Sara; Biricik, Anil; Nuccitelli, Andrea; Cotroneo, Ettore; Cottone, Giuliano; Kokocinski, Felix; Michel, Claude-Edouard; Minasi, Maria Giulia; Greco, Ermanno // Human Reproduction;Dec2014, Vol. 29 Issue 12, p2802 

    STUDY QUESTION Can next-generation sequencing (NGS) techniques be used reliably for comprehensive aneuploidy screening of human embryos from patients undergoing IVF treatments, with the purpose of identifying and selecting chromosomally normal embryos for transfer? SUMMARY ANSWER Extensive...

  • Legislative Progress on GMOs.  // Perfumer & Flavorist;Sep2015, Vol. 40 Issue 9, p10 

    The article focuses on approval of the Safe and Accurate Food Labeling Act of 2015 by House Agriculture Committee of United States as concerns by consumers to act against Genetically Modified Organisms.

  • Validation of the performance of a GMO multiplex screening assay based on microarray detection. Serge Leimanis; Sandrine Hamels; Florence Nazé; Guillaume Mbongolo Mbella; Myriam Sneyers; Rupert Hochegger; Hermann Broll; Lillian Roth; Klára Dallmann; Adrienn Micsinai; José La Paz; Maria Pla; Claudia Brünen-Nieweler; Nina Papazova; Isabel Taverniers; Norbert Hess; Britta Kirschneit; Yves Bertheau; Colette Audeon; Valérie Laval // European Food Research & Technology;Oct2008, Vol. 227 Issue 6, p1621 

    Abstract  A new screening method for the detection and identification of GMO, based on the use of multiplex PCR followed by microarray, has been developed and is presented. The technology is based on the identification of quite ubiquitous GMO genetic target elements first...

  • RIKEN Arabidopsis full‐length (RAFL) cDNA and its applications for expression profiling under abiotic stress conditions. Seki, Motoaki; Satou, Masakazu; Sakurai, Tetsuya; Akiyama, Kenji; Iida, Kei; Ishida, Junko; Nakajima, Maiko; Enju, Akiko; Narusaka, Mari; Fujita, Miki; Oono, Youko; Kamei, Ayako; Yamaguchi-Shinozaki, Kazuko; Shinozaki, Kazuo // Journal of Experimental Botany;Jan2004, Vol. 55 Issue 395, p213 

    Full‐length cDNAs are essential for the correct annotation of genomic sequences and for the functional analysis of genes and their products. 155 144 RIKEN Arabidopsis full‐length (RAFL) cDNA clones were isolated. The 3′‐end expressed sequence tags (ESTs) of all 155 144...

  • Non-Uniform Distribution Pattern for Differentially Expressed Genes of Transgenic Rice Huahui 1 at Different Developmental Stages and Environments. Zhi Liu; Jie Zhao; Yunhe Li; Wenwei Zhang; Guiliang Jian; Yufa Peng; Fangjun Qi // PLoS ONE;May2012, Vol. 7 Issue 5, p1 

    DNA microarray analysis is an effective method to detect unintended effects by detecting differentially expressed genes (DEG) in safety assessment of genetically modified (GM) crops. With the aim to reveal the distribution of DEG of GM crops under different conditions, we performed DNA...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics