TITLE

THE COMPARISON OF METHODS OF AN ASSESSMENT OF β-HYDROXYBUTYRATE ACID AND GLUCOSE IN BLOOD OF COWS

AUTHOR(S)
Kupczyński1, Robert; Adamski, Maciej; Pogoda-Sewerniak, Krystyna; Kuczaj, Marian; Zawadzki, Wojciech
PUB. DATE
January 2008
SOURCE
Zeszyty Naukowe Uniwersytetu Przyrodniczego we Wroclawiu: Biolog;2008, Vol. 56 Issue 566, p101
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
The aim of investigation was an evaluation of glucometer as rapid test marking change in β-hydroxybutyrate acid and glucose concentration in blood of cows fed on restrictive way. Moreover, the aim of this work was analyze of β-hydroxybutyrate acid and glucose content with use of different methods and on various biological materials (serum, plasma). The study was conducted on 20 cows, and 56 samples were collected. The highest increase in glucose level in blood was observed in 1st hour after silage administration, while in the case of β-HBA in 5th hour. That time should be taken into consideration in diagnostic study. An assessment of β-HBA with the use of Optium Xido glucometer is more reliable with higher content of β-HBA in blood. In the case of low content of β-HBA in blood, differences between analytical methods and glucometer were significant (p<0.01). Any differences between particular methods of glucose assessment were noted. A significant correlation between content of β-HB in full blood (glucometer) and serum (analytically), r=0.958, was observed. Similarly, a value of correlation coefficient for glucose was r = 0.739. High sensitivity and specificity prove the usefulness of Optium Xido glucometer in diagnosing of nutritional ketosis in tests over the clinic.
ACCESSION #
48441775

 

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