Kupczyński1, Robert; Adamski, Maciej; Pogoda-Sewerniak, Krystyna; Kuczaj, Marian; Zawadzki, Wojciech
January 2008
Zeszyty Naukowe Uniwersytetu Przyrodniczego we Wroclawiu: Biolog;2008, Vol. 56 Issue 566, p101
Academic Journal
The aim of investigation was an evaluation of glucometer as rapid test marking change in β-hydroxybutyrate acid and glucose concentration in blood of cows fed on restrictive way. Moreover, the aim of this work was analyze of β-hydroxybutyrate acid and glucose content with use of different methods and on various biological materials (serum, plasma). The study was conducted on 20 cows, and 56 samples were collected. The highest increase in glucose level in blood was observed in 1st hour after silage administration, while in the case of β-HBA in 5th hour. That time should be taken into consideration in diagnostic study. An assessment of β-HBA with the use of Optium Xido glucometer is more reliable with higher content of β-HBA in blood. In the case of low content of β-HBA in blood, differences between analytical methods and glucometer were significant (p<0.01). Any differences between particular methods of glucose assessment were noted. A significant correlation between content of β-HB in full blood (glucometer) and serum (analytically), r=0.958, was observed. Similarly, a value of correlation coefficient for glucose was r = 0.739. High sensitivity and specificity prove the usefulness of Optium Xido glucometer in diagnosing of nutritional ketosis in tests over the clinic.


Related Articles

  • Effect of Rectal Infusion of Short Chain Fatty Acids in Human Subjects. Wolever, Thomas M. S.; Brighenti, Furio; Royall, Dawna; Jenkins, Alexandra L.; Jenkins, David J. A. // American Journal of Gastroenterology;Sep1989, Vol. 84 Issue 9, p1027 

    Solutions containing 90 mmol sodium acetate plus 30 mmol sodium propionate (90 mM; isotonic), 180 mmol acetate plus 60 mmol propionate (180 mM; hypertonic) or an equivalent volume of isotonic saline were given over a 30-min period in random order by rectal infusion to six healthy subjects....

  • COMPARATIVE CLINICAL AND HAEMATOLOGICAL INVESTIGATIONS IN LACTATING COWS WITH SUBCLINICAL AND CLINICAL KETOSIS. Marutsova, Vania; Binev, Rumen; Marutsov, Plamen // Macedonian Veterinary Review;2015, Vol. 38 Issue 2, p159 

    Ketosis of lactating cows is among the most common metabolic diseases in modern dairy farms. The economic importance of the disease is caused by the reduced milk yield and body weight loss, poor feed conversion, lower conception rates, culling and increased mortality of affected animals. In the...

  • Untitled. Trickett, Sarah // Farmers Weekly;7/12/2013, Vol. 159 Issue 28, p79 

    The article previews the Keto-test for ketosis management in dairy cows from Elanco Animal Health.

  • Refining the Diagnostic Criteria for Diabetic Ketoacidosis. Sheikh-Ali, Mae; Karon, Bradley S.; Basu, Ananda; Kudva, Yogish C.; Muller, Lisa A.; Saenger, Amy K.; Schwenk, W. F.; Miles, John M. // Diabetes;Jun2007 Supplement 1, Vol. 56, pA136 

    Current consensus criteria for a diagnosis of diabetic ketoacidosis (DKA) include the presence of ketonuria/ketonemia, a serum bicarbonate (HCO[sub 3]) ≤ 18 mEq/L, a pH ≤7.30, and a glucose > 250 mg/dL. These diagnostic criteria have limitations, however. Measurement of ketone bodies...

  • Performance of equations for estimating glomerular filtration rate in diabetic patients. Šimetić, L.; Drmić, S.; Zibar, L.; Begić, I.; Šerić, V. // Biochemia Medica;2014 Supplement, Vol. 24, pS65 

    Background: Acetoacetate, glucose and hydroxybutyrate have been reported as interference in the Jaffe method for creatinine determination. They can be found in urine of diabetics. They could be responsible for overestimation of GFR by creatinine clearance (CrCl), or be a source for variability...

  • Chronic Central β-Hydroxybutyrate Infusion Improves Hepatic Insulin Resistance and Insulin Secretion in 900 Pancreatectomized Diabetic Rats. Park, Sunmin; Jin Sun Jang; Ji Eun Lee; Hye Ryeo Park; Ae Jin Jun; In Sook Jung // Diabetes;Jun2007 Supplement 1, Vol. 56, pA367 

    Some studies have shown that ketogenic diet has beneficial effect on regulation of body weight and glucose homeostasis in humans, but the effect of central ketone bodies on body weight and glucose homeostasis is not investigated. This study aimed to determine the effects and mechanism of...

  • Pharmacokinetic Properties of γ-Hydroxybutyrate (GHB) in Whole Blood, Serum, and Urine. Brailsford, Alan D.; Cowan, David A.; Kicman, Andrew T. // Journal of Analytical Toxicology;Mar2012, Vol. 36 Issue 2, p88 

    The article offers information on a study conducted on the pharmacokinetic properties of γ-Hydroxybutyrate (GHB) in serum, urine and whole blood. It states that the within-assay accuracy and average between assay precision was less than 15 percent for quality controls. It mentions that GHB...

  • Can Serum β-Hydroxybutyrate Be Used to Diagnose Diabetic Ketoacidosis? Sheikh-Ali, Mae; Karon, Brad S.; Basu, Ananda; Kudva, Yogish C.; Muller, Lisa A.; Xu, Jia; Schwenk, W. Frederick; Miles, John M. // Diabetes Care;Apr2008, Vol. 31 Issue 4, p643 

    OBJECTIVE -- Current criteria for the diagnosis of diabetic ketoacidosis (DKA) are limited by their nonspecificity (serum bicarbonate [HCO3] and pH) and qualitative nature (the presence of ketonemia/ketonuria). The present study was undertaken to determine whether quantitative measurement of a...

  • Studies on Certain Serum Metabolites in non Pregnant and Pregnant Bannur Ewes. Gururaj, P. M.; Veena, T.; Swamy, M. Narayana; Jayashankar, M. R.; Ramesh, P. T.; Shridhar, N. B. // Theriogenology Insight;Apr2014, Vol. 4 Issue 1, p1 

    A study was undertaken with the objective of making an insight in to the changes with respect to the level of certain metabolites in non pregnant and pregnant Bannur ewes which are indicative of their nutritional status and physiological well being. Eighteen Bannur ewes maintained under...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics