TITLE

Characterizing Polygala L. (Polygalaceae) Species in Southern Brazil Using ISSR

AUTHOR(S)
Lüdtke, Raquel; Agostini, Gustavo; Miotto, Silvia T. Sfoggia; Souza-Chies, Tatiana T.
PUB. DATE
June 2010
SOURCE
Plant Molecular Biology Reporter;Jun2010, Vol. 28 Issue 2, p317
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
The genus Polygala is one of the seven Polygalaceae genera that occur in the Brazilian flora, covering approximately 110 species. During the taxonomic review of Polygalaceae in Southern Brazil, difficulties were found when classifying species with very similar morphology, and morphological data alone could not clarify these interspecific relationships. In this context, inter-simple sequence repeat (ISSR) molecular markers were used in an attempt to characterize the genetic diversity and relationships among Polygala species. Nine Polygala species were analyzed using six selected ISSR primers that generated a total of 75 bands (100% polymorphic). The relationships were evaluated by dendrograms using the unweighted pair group method using arithmetic averages algorithm. The use of ISSR to solve the taxonomic problems was very useful for the Brazilian Polygala species. This is the first report of a molecular characterization of the Brazilian Polygala species to successfully group the different species. The ISSR results are in agreement with the morphological evidence of a new Polygala species from Southern Brazil.
ACCESSION #
48301632

 

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