Multiplex real-time PCR using SYBR® GreenER™ for the detection of DNA allergens in food

Pafundo, Simona; Gullì, Mariolina; Marmiroli, Nelson
March 2010
Analytical & Bioanalytical Chemistry;Mar2010, Vol. 396 Issue 5, p1831
Academic Journal
We describe the development of a six-target real-time multiplex PCR assay with the SYBR® GreenER™ fluorescent dye, targeted to genes encoding for allergenic proteins commonly present in many processed food products (patent application pending). The assay was successfully trialled on reconstructed food matrices and on a range of commercial foodstuffs, and is proposed as a ready-to-use analytical tool for food manufacturers to detect the presence or confirm the absence of sequences encoding for important allergenic proteins of plant origin. [Figure not available: see fulltext.]


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