TITLE

Multiplex real-time PCR using SYBR® GreenER™ for the detection of DNA allergens in food

AUTHOR(S)
Pafundo, Simona; Gullì, Mariolina; Marmiroli, Nelson
PUB. DATE
March 2010
SOURCE
Analytical & Bioanalytical Chemistry;Mar2010, Vol. 396 Issue 5, p1831
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
We describe the development of a six-target real-time multiplex PCR assay with the SYBR® GreenER™ fluorescent dye, targeted to genes encoding for allergenic proteins commonly present in many processed food products (patent application pending). The assay was successfully trialled on reconstructed food matrices and on a range of commercial foodstuffs, and is proposed as a ready-to-use analytical tool for food manufacturers to detect the presence or confirm the absence of sequences encoding for important allergenic proteins of plant origin. [Figure not available: see fulltext.]
ACCESSION #
48191149

 

Related Articles

  • Detection allergen soybean in food using real - time PCR. Shu - ya ZHANG; Cui YU; Qing SONG; Zhuo - ran YU; Qin GAO // Chinese Journal of Oil Crop Sciences;2012, Vol. 34 Issue 6, p661 

    A real - time PCR method was established for detecting soybean DNA in food by using primers and probe corresponding to soybean immunodominant allergen P34. The method was specific for soybean and did not show any cross - reactivity with common food ingredients. The limit of detection was...

  • Sensitive DNA-based allergen detection depends on food matrix and DNA isolation method. Kenk, Marion; Panter, Silvia; Engler-Blum, Gabriele; Bergemann, Jörg // European Food Research & Technology;Feb2012, Vol. 234 Issue 2, p351 

    Performance of DNA-based methods used for allergen detection does not only depend on the real-time PCR system, but also on the DNA isolation method used. Comparison of different isolation methods showed that yield and purity of isolated DNA strongly depends on the isolation method deployed as...

  • Two tetraplex real-time PCR for the detection and quantification of DNA from eight allergens in food. Köppel, René; Dvorak, Veronika; Zimmerli, Franziska; Breitenmoser, Alda; Eugster, Albert; Waiblinger, Hans-Ulrich // European Food Research & Technology;Jan2010, Vol. 230 Issue 3, p367 

    According to the EU and Swiss legislation, food has to be labelled for allergens to enable allergic consumers to avoid such food and its products. To provide efficient and reliable methods, two novel quantitative multiplex real-time polymerase chain reaction systems were developed and validated....

  • uMELT: prediction of high-resolution melting curves and dynamic melting profiles of PCR products in a rich web application. Dwight, Zachary; Palais, Robert; Wittwer, Carl T. // Bioinformatics;Apr2011, Vol. 27 Issue 7, p1019 

    Summary: uMeltSM is a flexible web-based tool for predicting DNA melting curves and denaturation profiles of PCR products. The user defines an amplicon sequence and chooses a set of thermodynamic and experimental parameters that include nearest neighbor stacking energies, loop entropy effects,...

  • Molecular Studies on Heat Shock Protein 70 Gene (Hsp70) Using ClustalW Multiple Sequence Alignment.  // Journal of Life Sciences;Oct2010, Vol. 4 Issue 10, p1 

    No abstract available.

  • A novel FRET pair for detection of parallel DNA triplexes by the LightCycler. Schneider, Uffe V.; Severinsen, Jette K.; Géci, Imrich; Okkels, Limei M.; Jøhnk, Nina; Mikkelsen, Nikolaj D.; Klinge, Teena; Pedersen, Erik B.; Westh, Henrik; Lisby, Gorm // BMC Biotechnology;2010, Vol. 10, Special section p1 

    Background: Melting temperature of DNA structures can be determined on the LightCycler using quenching of FAM. This method is very suitable for pH independent melting point (Tm) determination performed at basic or neutral pH, as a high throughput alternative to UV absorbance measurements. At...

  • Simultaneous Screening of 24 Target Genes of Foodborne Pathogens in 35 Foodborne Outbreaks Using Multiplex Real-Time SYBR Green PCR Analysis. Fukushima, Hiroshi; Kawase, Jun; Etoh, Yoshiki; Sugama, Kumiko; Yashiro, Shunshuke; Iida, Natsuko; Yamaguchi, Keiji // International Journal of Microbiology;2010, p1 

    A set of 8 multiplex real-time SYBR Green PCR (SG-PCR) assays including 3 target primers and an internal amplification control (IAC) primer was simultaneously evaluated in 3 h or less with regard to detection of 24 target genes of 23 foodborne pathogens in 7 stool specimens of foodborne outbreak...

  • Thermal equivalence of DNA duplexes without calculation of melting temperature. Weber, Gerald; Haslam, Niall; Whiteford, Nava; Prügel-Bennett, Adam; Essex, Jonathan W.; Neylon, Cameron // Nature Physics;Jan2006, Vol. 2 Issue 1, p55 

    The common key to nearly all processes involving DNA is the hybridization and melting of the double helix: from transmission of genetic information and RNA transcription, to polymerase chain reaction and DNA microarray analysis, DNA mechanical nanodevices and DNA computing. Selecting DNA...

  • Fluorescent Duplex Allele-Specific PCR and Amplicon Melting for Rapid Homogeneous mtDNA Haplogroup H Screening and Sensitive Mixture Detection. Niederstätter, Harald; Parson, Walther // PLoS ONE;2009, Vol. 4 Issue 12, p1 

    Background: For large scale studies aiming at a better understanding of mitochondrial DNA (mtDNA), sequence variation in particular mt haplogroups (hgs) and population structure, reliable low-cost high-throughput genotyping assays are needed. Furthermore, methods facilitating sensitive mixture...

Share

Read the Article

Courtesy of THE LIBRARY OF VIRGINIA

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics