The 1000 bar and 24 hour limits of one-dimensional HPLC – graphical representations

Meyer, Veronika R.
March 2010
Analytical & Bioanalytical Chemistry;Mar2010, Vol. 396 Issue 5, p1633
Academic Journal
The article explains the 1000 bar and 24-hour limits of one dimensional high performance liquid chromatography (HPLC). It describes the separation performance associated with deviations from the optimum flux velocity of the eluent. The empirical van Deemter equation is discussed, along with the plots created by researchers István Halász in 1982 and Hans Poppe in 1997. How the limits affect HPLC performance is also explained.


Related Articles

  • The Thermally Tuned Tandem Column Approach to Optimizing Selectivity in HPLC. Yun Mao; Carr, Peter W. // LC-GC North America;Feb2003, Vol. 21 Issue 2, p150 

    Describes the thermally tuned tandem column approach to optimizing selectivity in high performance liquid chromatography. Connection of two columns with different chromatographic selectivities; Independent temperature control for the columns; Strategies for single-column optimization.

  • The Use of Micron-Sized Particles in Ultrahigh-Pressure Liquid Chromatography. Jerkovich, Anton D.; Scott Mellors, J.; Jorgenson, James W. // LC-GC Europe;Jun2003, Vol. 16 Issue 6a, p20 

    Analyzes the use of micron-sized particles in ultrahigh-pressure liquid chromatography (HPLC). Effects of conventional HPLC-type pressures on analyte retention in reversed-phase HPLC; Efficacy of micron-sized particles in increasing the speed and resolution in reversed-phase liquid chromatography.

  • Stability-Indicating HPLC Method Development--A Systematic Approach Using pH and Column Selectivity.  // LC-GC North America;02/01/2002 Supplement, Vol. 20 Issue 2, p29 

    Focuses on the high performance liquid chromatography (HPLC) stability-indicating assay. Information on the HPLC method development approach; Determination of purity of parent peak using photodiode array spectral analysis; List of compounds for chromatographic application of the HPLC method.

  • Faster Analysis and Higher Efficiency with Thermally Stable HPLC Column. Stoll, Dwight; Carr, Peter W. // LC-GC North America;02/01/2002 Supplement, Vol. 20 Issue 2, p30 

    Focuses on the use of the ZirChrom�-PBD column for high performance liquid chromatography. Background on the analysis of basic compounds at elevated temperature; Advantage of separating samples at high temperature; Use of the ZirChrom�-PBD column in separating antihistamines.

  • CALENDAR.  // LC-GC North America;Jan2011, Vol. 29 Issue 1, p90 

    A calendar of chromatographic analysis-related events in 2011 in the U.S and some parts abroad is presented including the International Symposium on Capillary Chromatography and Electrophoresis in San Diego, California, the Triangle Chromatography Symposium in Raleigh, North Carolina, and the...

  • Understanding Gradient HPLC.  // LC-GC North America;Jul2013, Vol. 31 Issue 7, p578 

    An excerpt from the article "Understanding Gradient HPLC" from the online publication of the periodical is presented.

  • A 'New' Injection Mode for Preparative SFC. Speybrouck, D.; Jolly, S.; Thomas, S.; Corens, D. // International Labmate;Mar2015, Vol. 40 Issue 2, p4 

    The method of injecting samples in preparative supercritical fluid chromatography (Prep SFC) is a crucial and critical process. In SFC, unlike HPLC, it is not possible to dissolve the sample in the mobile phase, which consists mainly of CO2. The sample is therefore dissolved in the mobile phase...

  • Secrets to Successfully Translating and Transferring HPLC Methods.  // LC-GC North America;Sep2013, Vol. 31 Issue 9, p822 

    The article focuses on transfer and translation of high performance liquid chromatography (HPLC) methods to a different instrument. It informs that the stationary phase chemistry does not change between the original and translated methods during the process of translation. It also informs about...

  • Current Trends in HPLC Column Usage. Majors, Ronald E. // LC-GC North America;Jun2007, Vol. 25 Issue 6, p532 

    The article focuses on the 2007 web-based High Performance Liquid Chromatography (HPLC) survey in North America. He uses the results of the survey to chart trends in column technology and the practice of HPLC. Several topics are discussed, including particle sizes in analytical preparative...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics