Determination of diketopiperazines of Burkholderia cepacia CF-66 by gas chromatography–mass spectrometry

Jian-Hua Wang; Chun-Shan Quan; Xiao-Hui Qi; Xin Li; Sheng-Di Fan
March 2010
Analytical & Bioanalytical Chemistry;Mar2010, Vol. 396 Issue 5, p1773
Academic Journal
Bacteria communicate with each other by a process termed “quorum sensing” (QS), and diffusible, low-molecular-weight chemicals, called signal molecules, are used as the communication languages. In cell-free Burkholderia cepacia CF-66 culture supernatants, five compounds suspected of being signal molecules were identified. The gene ( cepI) related with AHLs synthesis were not detected by polymerase chain reaction (PCR) using specific primers. Gas chromatography–mass spectrometry (GC–MS) revealed that these compounds were not AHLs but the diketopiperazines (DKPs) cyclo(Pro–Phe), cyclo(Pro–Tyr), cyclo(Ala–Val), cyclo(Pro–Leu), and cyclo(Pro–Val), all of which were both d and l-type. Four kinds of DKPs had been isolated from other Gram-negative bacteria, but the other was a novel kind discovered in CF-66, and l-cyclo (Pro–Phe) was quantified by GC–MS. It was found that exogenous DKPs had a negative effect on the candidacidal activity of the culture supernatant extracts.


Related Articles

  • Functional characterization and differential expression studies of squalene synthase from Withania somnifera. Gupta, Neha; Sharma, Poonam; Santosh Kumar, R.; Vishwakarma, Rishi; Khan, B. // Molecular Biology Reports;Sep2012, Vol. 39 Issue 9, p8803 

    Squalene synthase (SQS: EC is a potential branch point regulatory enzyme and represents the first committed step to diverge the carbon flux from the main isoprenoid pathway towards sterol biosynthesis. In the present study, cloning and characterization of Withania somnifera squalene...

  • Regional growth strategy is the key.  // BioSpectrum;Jul2012, Vol. 10 Issue 7, p55 

    The article profiles India-based Thermo Fisher Scientific Inc. which has attained a majority market share across segments such as mass spectrometry, gas chromatography and centrifuges. It notes that the company has successfully integrated its global acquisitions in the country which include...

  • PEAKS of Interest.  // LC-GC North America;Mar2011, Vol. 29 Issue 3, p214 

    The article offers briefs accounts of various research related to chromatographic analysis. The method that analysis dioxins in animal feed and tissue with the use of high resolution gas chromatography with mass spectrometry detection (GC-MS) has been published. The role of quorum sensing on an...

  • Single drop microextraction of homoserine lactones based quorum sensing signal molecules, and the separation of their enantiomers using gas chromatography mass spectrometry in the presence of biological matrices. Malik, Ashok Kumar; Fekete, Agnes; Gebefuegi, Istvan; Rothballer, Michael; Schmitt-Kopplin, Philippe // Microchimica Acta;Jul2009, Vol. 166 Issue 1/2, p101 

    N-acylated homoserine lactones (AHLs) are produced by Gram-negative bacteria as communication signals and are frequently studied as mediators of the “quorum sensing” response of bacterial communities. AHLs are optically active components and the L-form is known to have activity in...

  • Anti-quorum sensing potential of the mangrove Rhizophora annamalayana. Musthafa, Khadar; Sahu, Sunil; Ravi, Arumugam; Kathiresan, Kandasamy // World Journal of Microbiology & Biotechnology;Oct2013, Vol. 29 Issue 10, p1851 

    The present study was carried out to assess the anti-quorum sensing (anti-QS) activity of bark extract obtained from the mangrove plant Rhizophora annamalayana Kathir. against Gram-negative bacteria. In microtitre plate assay, the bark extract at a concentration of 1 mg/ml inhibited the...

  • Executives...FYI.  // Emerging Food R&D Report;Apr2013, Vol. 24 Issue 1, p8 

    The article offers information regarding two studies related to potential probiotic property of table olive, and polyunsaturated fatty acid (PUFA) in sea urchin. It says that the probiotic characteristics of green olive brines were examined using methods including multiple polymerase chain...

  • Temperature has reciprocal effects on colanic acid and polysialic acid biosynthesis in E. coli K92. Navasa, Nicolás; Rodríguez-Aparicio, Leandro; Martínez-Blanco, Honorina; Arcos, Mario; Ferrero, Miguel // Applied Microbiology & Biotechnology;Mar2009, Vol. 82 Issue 4, p721 

    Escherichia coli K92 is an opportunistic pathogen bacterium able to produce polysialic acid (PA) capsules when grows at 37°C. PA polysaccharides are cell-associated homopolymers tailored from acid sialic monomers that function as virulence factors in different neuroinvasive diseases caused by...

  • Effects of Methyl Jasmonate and an Endophytic Fungus on Plant Resistance to Insect Herbivores. Thomas Bultman; T. Sullivan // Journal of Chemical Ecology;Dec2008, Vol. 34 Issue 12, p1511 

    Abstract  Tall fescue (Lolium arundinaceum) forms a mutualistic relationship with the fungal endophyte Neotyphodium coenophialum. The endophyte provides constitutive resistance to herbivores through its production of alkaloid compounds. Moreover, herbivore attack induces elevated...

  • An ethylene response factor OsWR1 responsive to drought stress transcriptionally activates wax synthesis related genes and increases wax production in rice. Wang, Youhua; Wan, Liyun; Zhang, Lixia; Zhang, Zhijin; Zhang, Haiwen; Quan, Ruidang; Zhou, Shirong; Huang, Rongfeng // Plant Molecular Biology;Feb2012, Vol. 78 Issue 3, p275 

    Increasing evidence has revealed the major enzymes-involved in Arabidopsis and maize wax/cutin synthesis; however, there is limited information about the genes-associated with wax/cutin synthesis in rice. Here we report the characterization of an ethylene response factor gene in rice. This rice...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics