TITLE

Detection of hazelnuts and almonds using commercial ELISA test kits

AUTHOR(S)
Garber, Eric A. E.; Perry, Jesse
PUB. DATE
March 2010
SOURCE
Analytical & Bioanalytical Chemistry;Mar2010, Vol. 396 Issue 5, p1939
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Three commercial sandwich enzyme-linked immunosorbent assay (ELISA) test kits for the detection of hazelnuts and almonds were evaluated. Limits of detection and dynamic ranges were determined for hazelnuts and almonds spiked into cooked oatmeal, dipping chocolate, and muffins (baked). The limit of detection values varied from 1 to 38 μg/g, depending on the food matrix and ELISA test kit. Percent recoveries based on the standards supplied with the test kits varied from 10% to 170%. It is impossible to ascertain whether the percent recoveries reflect the performance of the ELISAs or differences between the protein content of the nuts used to spike the samples and the test kit standards. Unfortunately, reference materials do not exist that can be used to compare the results from different test kits and standardize the test kit standards. Also, insufficient knowledge regarding the epitope specificity of the antibodies used in the ELISAs further hinders interpretation of the results generated by the different test kits. [Figure not available: see fulltext.]
ACCESSION #
48191122

 

Related Articles

  • Quantitative analysis of shrimp allergen in food matrices using a protein chip based on sandwich immunoassay. Li Zhenxing; Zhang Yiqun; Lin Hong; Samee Haider; Jiang Jie // European Food Research & Technology;May2010, Vol. 231 Issue 1, p47 

    Food allergy is increasingly becoming a serious concern these days. With packaged foods becoming the norm of the day, food allergy cases out of accidental consumption are becoming rampant, thereby generating great risks for the subjects involved and prompting food authorities in different...

  • Proficiency testing for quality assurance of allergens methods. Owen, Linda; Gilbert, John // Analytical & Bioanalytical Chemistry;Sep2009, Vol. 395 Issue 1, p147 

    Proficiency testing is an external quality control check, whereby the quality of an analytical result is checked against criteria that are set independently of the laboratory carrying out the analysis. Participants in a proficiency test are encouraged to use the method of their choice to...

  • Allergen immunoassays—considerations for use of naturally incurred standards. Taylor, Steve L.; Nordlee, Julie A.; Niemann, Lynn M.; Lambrecht, Debra M. // Analytical & Bioanalytical Chemistry;Sep2009, Vol. 395 Issue 1, p83 

    The enzyme-linked immunosorbent assay (ELISA) offers many advantages for the detection of potentially hazardous allergenic food residues that might become adventitious components of other foods during the course of food production and processing. ELISAs detect proteins, and food allergens are...

  • Commercial lateral flow devices for rapid detection of peanut ( Arachis hypogaea) and hazelnut ( Corylus avellana) cross-contamination in the industrial production of cookies. Röder, Martin; Vieths, Stefan; Holzhauser, Thomas // Analytical & Bioanalytical Chemistry;Sep2009, Vol. 395 Issue 1, p103 

    Lateral flow devices (LFDs) are qualitative immunochromatographic tests for the rapid and specific detection of target analytes. We investigated commercially available LFDs for their ability to detect potentially allergenic peanut and hazelnut in raw cookie dough and chocolate, two important...

  • Advanced Analysis of Allergens in Foods: LC-MS-MS Methodologies and Future Insights. Bailey, Lauryn; Lock, Steven; Rummel, Michael; Pike, Erica // Spectroscopy;Mar2012 Supplement, p28 

    The article discusses on methods for advanced analysis of food allergens. It mentions that traditional techniques based on use on polymerase chain reaction (PCR) and enzyme-linked immunosorbent assay are susceptible to false negative results. It informs that the new technique based on use of...

  • Comparison of the immunogenicity of yak milk and cow milk. Li, Haimei; Ma, Ying; Xiang, Junjian; Xu, Guilian; Chen, Lishui; Cheng, Jinju; Wang, Jiaqi; Li, Qiming; He, Shenghua // European Food Research & Technology;Oct2011, Vol. 233 Issue 4, p545 

    This is a comparison of potential allergenicity in a mouse model. Balb/C mice were sensitized by intraperitoneal injections (administered in three doses) of skimmed milk, casein, and whey protein from cow or yak milk. After 4 weeks, the mice were challenged and killed, sera were collected, and...

  • Peanut Allergen (Ara h 1) Detection in Foods Containing Chocolate. Pomés, A.; Vinton, R.; Chapman, M.D. // Journal of Food Protection;Apr2004, Vol. 67 Issue 4, p793 

    Inadvertent exposure to peanut in foods poses health risks for peanut-allergic individuals that can be reduced by improving detection systems for allergen contaminants in food products and manufacturing processes. Detection of peanut in chocolate has been especially difficult. We report the...

  • Magnetic particles functionalized with PAMAM-dendrimers and antibodies: a new system for an ELISA method able to detect Ara h3/4 peanut allergen in foods. Speroni, Francesca; Elviri, Lisa; Careri, Maria; Mangia, Alessandro // Analytical & Bioanalytical Chemistry;Aug2010, Vol. 397 Issue 7, p3035 

    An innovative enzyme-linked immunosorbent assay (ELISA) format based on antibody-coated magnetic micro-particles (MPs) for the sensitive detection of Ara h3/4 allergen in food is described. The immunosupport is suspended in the incubation solutions and the MPs with the captured allergen can be...

  • Development and in-house validation of allergen-specific ELISA tests for the quantification of Dau c 1.01, Dau c 1.02 and Dau c 4 in carrot extracts ( Daucus carota). Foetisch, Kay; Dahl, Lotte; Jansen, Baerbel; Becker, Wolf-Meinhard; Lidholm, Jonas; Van Ree, Ronald; Broll, Hermann; Kaul, Susanne; Vieths, Stefan; Holzhauser, Thomas // Analytical & Bioanalytical Chemistry;Jan2011, Vol. 399 Issue 2, p935 

    Even though carrot allergy is common in Europe, the amount of different allergens in carrots is still unknown due to a lack of methods for quantitative allergen measurements. The current study aimed at the development of quantitative ELISA tests for the known carrot allergens, namely Dau c 1.01,...

Share

Read the Article

Courtesy of VIRGINIA BEACH PUBLIC LIBRARY AND SYSTEM

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics