TITLE

Evaluation of Three Different Methods to Establish Animal Models of Acanthamoeba Keratitis

AUTHOR(S)
Meiyu Ren; Xinyi Wu
PUB. DATE
January 2010
SOURCE
Yonsei Medical Journal;1/1/2010, Vol. 51 Issue 1, p121
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Purpose: To produce animal models of Acanthamoeba keratitis and to evaluate the advantages and adaptation range of each of the three methods employed. Materials and Methods: Mice and Wistar rats in three groups of 15 rats and 15 mice each were used to establish the models. Right comeas in group A were scratched and challenged with Acanthamoeba. Those in group B were scratched and covered with contact lenses incubated with Acanthamoeba. Those in group C received an intrastromal injection of Acanthamoeba. Five rats and 5 mice in each group were used for histopathological investigations and the other 10 in each group were used for clinical evaluation. The models were evaluated by slit lamp examination, microscopic examination and culture of corneal scrapings, HE staining of comeal sections, and pathological scoring of the infections. Results: Four rats and 6 mice in group A, 7 rats and 8 mice in group B, and 10 rats and 10 mice in group C developed typical Acanthamoeba keratitis. Conclusion: Comeal scratching alone has the lowest infection rate, while scratching and then covering with contaminated contact lenses has a moderate rate of infection and most closely mimics what happens in most human infections. Intrastromal injection of Acanthamoeba gives a much higher infection rate and more severe Acanthamoeba keratitis.
ACCESSION #
48169149

 

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