Evaluation of Three Different Methods to Establish Animal Models of Acanthamoeba Keratitis

Meiyu Ren; Xinyi Wu
January 2010
Yonsei Medical Journal;1/1/2010, Vol. 51 Issue 1, p121
Academic Journal
Purpose: To produce animal models of Acanthamoeba keratitis and to evaluate the advantages and adaptation range of each of the three methods employed. Materials and Methods: Mice and Wistar rats in three groups of 15 rats and 15 mice each were used to establish the models. Right comeas in group A were scratched and challenged with Acanthamoeba. Those in group B were scratched and covered with contact lenses incubated with Acanthamoeba. Those in group C received an intrastromal injection of Acanthamoeba. Five rats and 5 mice in each group were used for histopathological investigations and the other 10 in each group were used for clinical evaluation. The models were evaluated by slit lamp examination, microscopic examination and culture of corneal scrapings, HE staining of comeal sections, and pathological scoring of the infections. Results: Four rats and 6 mice in group A, 7 rats and 8 mice in group B, and 10 rats and 10 mice in group C developed typical Acanthamoeba keratitis. Conclusion: Comeal scratching alone has the lowest infection rate, while scratching and then covering with contaminated contact lenses has a moderate rate of infection and most closely mimics what happens in most human infections. Intrastromal injection of Acanthamoeba gives a much higher infection rate and more severe Acanthamoeba keratitis.


Related Articles

  • What Are the Odds? Sindt, Christine W. // Review of Optometry;Sep2007 Supplement2, p12 

    The article discusses the risk factors affecting the use of contact lenses which include modality of wear, number of organisms present, and corneal integrity. It also discusses the importance of proper care and regular examinations to avoid the risk of Acanthamoeba infection. It relates that...

  • Lethal Effects of Helianthemum lippii (L.) on Acanthamoeba castellanii Cysts in Vitro. Badria, F. A.; Hetta, M. H.; Sarhan, Rania M.; Ezz El-Din, H. M. // Korean Journal of Parasitology;Jun2014, Vol. 52 Issue 3, p243 

    Acanthamoeba spp. commonly cause Acanthamoeba keratitis which is typically associated with the wear of contact lenses. Therefore, finding an economic, efficient, and safe therapy of natural origin is of outmost importance. This study examined the in vitro lethal potential of ethyl acetate and...

  • Acanthamoeba keratitis increasing at alarming rate. Guttman, Cheryl // Ophthalmology Times;1/1/2006, Vol. 31 Issue 1, p1 

    The article reports on the alarming increase on the rate of acanthamoeba keratitis cases in the U.S. Ophthalmologist Fabiano N. Rocha reported that 19 patients has this infection diagnosed between January 2004 and August 2005. Those patients were said to have histories of frequent-replacement...

  • Results of case-control studies supportthe association between contact lens use and Acanthamoeba keratitis. Pacella, Elena; La Torre, Giuseppe; De Giusti, Maria; Brillante, Chiara; Lombardi, Anna Maria; Smaldone, Gianpaolo; Lenzi, Tommaso; Pacella, Fernanda // Clinical Ophthalmology;May2013, Vol. 7, p991 

    Background: Acanthamoeba keratitis (AK) is ever more frequently reported in industrialized countries. The loss of the corneal surface integrity consequent to secondary microtrauma produced by the use of contact lens (CL) favors the penetration of the parasite into the corneal tissue. Objectives:...

  • Effect of caspofungin on trophozoites and cysts of three species of Acanthamoeba. Sabrina Bouyer; Christine Imbert; Gyslaine Daniault; Estelle Cateau; Marie-Hélène Rodier // Journal of Antimicrobial Chemotherapy (JAC);Jan2007, Vol. 59 Issue 1, p122 

    Objectives: Amoebic keratitis is difficult to treat, without total efficacy in some patients because of cysts that are less susceptible than trophozoites to the usual treatments. We investigated here the in vitro effectiveness of caspofungin, a new antifungal, against three species of...

  • AK: Let's Shift the Blame. Abelson, Mark B.; Tobey, Caroline // Review of Optometry;5/15/2012 Review of Cornea & Contac, p8 

    The article discusses the things to consider in managing Acanthamoeba keratitis (AK) in patients with contact lens. It provides an overview about the bacteria, Acanthamoeba, and the disease's correlation with contact lens. It emphasizes the need for medical practitioners to educate their...

  • Acanthamoeba Keratitis.  // Review of Optometry;Jan2011 The Corneal Atlas, p7 

    The article focuses on the characteristics and causes of acanthamoeba keratitis and treatments that are associated with the disorder. According to the article, the inflammation was thought to be first associated with contact lens wearers in 1970s, however it was discovered that acanthamoeba...

  • Acanthamoeba Keratitis. Shafer, Kathy // Review of Optometry;Oct2006 Supplement, Vol. 143, p16 

    The article discusses the increasing incidence of Acanthamoeba Keratitis (AK) in the U.S. The American Optometric Association issued guidelines for contact lens users that would protect their eyes from infections like AK. The University of Illinois Eye Center website contained electronic survey...

  • What is Acanthamoeba Keratitis? Wilcox, Mark // Review of Optometry;2/15/2008 Part 1 of 3, Vol. 145 Issue 2, Special section p3 

    The article focuses on the nature of the Acanthamoeba Keratitis (AK) which causes eye infection. Acanthamoeba is a free-living protozoan which is commonly found in tap water, ponds, rivers and swimming pools and is the agent of AK. AK is less common than fungal and bacterial keratitis and...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics