TITLE

EFFECTS OF ALUMINUM ON DELTA AMINOLEVULINIC ACID DEHYDRATASE IN VIVO AND IN VITRO

AUTHOR(S)
Baranauskienė, Dalė; Stumylaitė, Loreta; Kreg៮dytė, Rima; Ryselis, Stanislovas; Abdrakhmanovas, Olegas; Stepaniukas, Andrius
PUB. DATE
December 2009
SOURCE
Veterinarija ir Zootechnika;2009, Vol. 48 Issue 70, p3
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
The present study examined the effect of aluminum on δ-aminolevulinic acid dehydratase (δ-ALAD) and hematocrit, and assessed the effects of zinc and selenium on activity of the enzyme affected by aluminum in vivo and in vitro. Experiments were done on white laboratory mice of (20-25) g body mass. To assess the effect of aluminum on δ- ALAD in vivo, mice injected i.p. with 0.5 LD50 aluminum chloride (AlCl3) (25 mg Al3+/kg body mass). To estimate the effect of zinc and selenium on activity of the enzyme affected by aluminum, twenty minutes before intoxication with 0.5 LD50 aluminum chloride mice were injected i.p. with 0.5 LD50 of sodium selenite (Na2SeO3) or with 1.56 mg/kg of zinc sulphate (ZnSO4). Control animals received an injection of the same volume of saline. Injection of mice with a single dose of aliuminium significantly increased concentration of metal in blood. However, δ-ALAD activity changed only slightly. Furthermore, addition of zinc before aluminum injection was related to significant increase of aluminum content and a little enhancement of δ-ALAD activity in blood. In blood of mice where selenium additives were used no changes in aluminum concentration or δ-ALAD activity was registered, and level of hematocrit decreased. The in vitro effects of aluminum on δ-ALAD activity in blood of experimental mice were investigated. Concentration causing half-maximal inhibition (IC50) of enzyme activity was used to assess the effects of Al3+ on δ-ALAD activity in blood. The findings suggested that low concentrations of aluminum ions slightly decrease δ-ALAD activity in vitro, while high concentrations of aluminum ions inhibited the enzyme. Aluminum ions are medium whereas zinc ions are weak and cadmium ions are strong catalytic poison (IC50 Cd2+ < IC50 Al3+ < IC50 Zn2+). Zinc ions also showed a weak protective effect on inhibition of δ-ALAD caused by aluminum ions, but do not remove it ((IC50 Al3+ < IC50 Al3+ + Zn2+).
ACCESSION #
48001897

 

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