Cryopreservation of feline epididymal spermatozoa from dead and alive animals and its use in assisted reproduction

Cocchia, N.; Ciani, F.; El-Rass, R.; Russo, M.; Borzacchiello, G.; Esposito, V.; Montagnaro, S.; Avallone, L.; Tortora, G.; Lorizio, R.
February 2010
Zygote;Feb2010, Vol. 18 Issue 1, p1
Academic Journal
Cryopreservation of gametes is an important tool in assisted reproduction programmes; long-term storage of oocytes or spermatozoa is necessary when in vitro fertilization (IVF) or artificial insemination is to be performed at a future date. Cryopreservation of epididymal spermatozoa offers a potential tool for rescuing genetic material from males of endangered populations. The objectives of this work were to: (1) examine sperm motility, viability, abnormality and acrosome integrity of frozen-thawed domestic cat epididymal spermatozoa; and (2) evaluate the same cryopreservation method on wild feline spermatozoa, needed to preserve their genetic resources. Epididymides were collected from 20 domestic cats during routine neutering procedure and from two wild felines at autopsy. The sperm samples, diluted with 4% glycerol/Tris/egg yolk, were loaded into 0.25 ml mini-straws, exposed to nitrogen vapour and stored in liquid nitrogen. After 4 weeks, samples were thawed and re-evaluated. The quality of each fresh and frozen-thawed sperm sample was tested by determining the motility (54.7±11.3% and 32±13.1% respectively for cat spermatozoa; 38.3±18.7% and 21.5±16.8% respectively for tiger spermatozoa), viability (74.3±8.6% and 45.2±9.4% respectively for cat spermatozoa; 42.4±14.5% and 33.5±12.9% respectively for wild felid spermatozoa), morphology and acrosomal status. The present study showed that feline epididymal spermatozoa can be frozen in egg-yolk extender with 4.0% glycerol in 0.25 ml straws. The procedure used in the present study for epididymal cat sperm cryopreservation may be applied to bank the genetic resources of wild felid species.


Related Articles

  • Impact on Hyperactivated Motility of Cryopreserved Mouse Sperm from Pretreatment with Thimerosal. Yan-Ping Wang; Guang-Bin Zhou; Yan Zeng; Jun-Jie Li; Qing-Jing Zhang; Yun-Peng Hou; Shi-En Zhu // Asian Journal of Animal & Veterinary Advances;Nov2011, Vol. 6 Issue 11, p1052 

    Hyperactivated motility of sperm cell is essentials to successful fertilization. This study was performed to observe the effect of Thimerosal treatment of cryopreserved mouse sperm on their hyperactivated motility and subsequent development to two-cell embryos after in vitro fertilization....

  • Changes in Motility Characteristics of Goat Spermatozoa During Glycerol-Equilibration and the Relevance to Cryopreservation. Sundararaman, M. N.; Edwin, M. J. // Asian Journal of Cell Biology;2008, Vol. 3 Issue 1, p22 

    Artificial Insemination (AI) with frozen Boer goat semen could be a viable option for genetic upgradation of non-descript local goats. To maximize the utilization of Boer germplasm, minimizing the losses due to poor freezability and improving the quality of frozen semen are the areas to be...

  • Freezing of Spermatozoa from Tigaie Rams Belonging to the Hill Ecotype. Miclea, Vasile; Zăhan, Marius; Nagy, Ştefan; Miclea, Ileana; Rusu, Alexandru; Varo-Ghiuru, Florin // Scientific Papers: Animal Science & Biotechnologies / Lucrari St;Jun2011, Vol. 44 Issue 1, p294 

    Our purpose was to study the influence of freezing on the viability and frequency of abnormalities in frozen ram spermatozoa. Sperm was collected form 20 rams belonging to the hill ecotype of the Tigaie breed using the artificial vagina technique and volume and motility were assessed. Afterward...

  • Single sperm cryopreservation on cryoloops. Desai, Nina N; Blackmon, Heather; Goldfarb, James // Reproductive BioMedicine Online (Reproductive Healthcare Limited;Jul2004, Vol. 9 Issue 1, p47 

    Methodology enabling cryopreservation of individual spermatozoa in extreme cases of oligozoospermia would tremendously benefit patients. This study explores the use of a nylon loop for cryopreservation of small quantities of spermatozoa, and also describes a novel technique for freezing...

  • The effect of different extenders on the motility and morphology of ram sperm frozen or stored at 4 °C. Kulaksiz, Recai; Çebi, Çiğdem; Akçay, Ergun // Turkish Journal of Veterinary & Animal Sciences;Apr2012, Vol. 36 Issue 2, p177 

    This study was conducted to evaluate the effects on sperm motility and abnormality in ram semen extended with skimmed milk (M), sodium citrate (SC), Tris (T), and Bioxcell® (B) aft er storage in liquid form at 4 °C and in frozen form. Ejaculates were collected from 3 Akkaraman rams by...

  • Sperm preparation before freezing improves sperm motility and reduces apoptosis in post-freezing-thawing sperm compared with post-thawing sperm preparation. Petyim, Somsin; Neungton, Chanon; Thanaboonyawat, Isarin; Laokirkkiat, Pitak; Choavaratana, Roungsin // Journal of Assisted Reproduction & Genetics;Dec2014, Vol. 31 Issue 12, p1673 

    Purpose: To evaluate whether sperm preparation (swim-up technique) before freezing improves the percentages of sperm motility, sperm viability, and non-apoptotic spermatozoa after freezing-thawing process compared with preparation after cryopreservation. Methods: Semen samples from 65 infertile...

  • Effect of different extenders on quality of frozen-thawed boar semen. Frydrychová, S.; Lustyková, A.; Václavková, E.; Lipenský, J.; Rozkot, M. // Indian Journal of Animal Research;Dec2015, Vol. 49 Issue 6, p851 

    The objective of this study was to investigate the effect of using different extenders viz. Androhep, Safecell Plus and SUS during cryopreservation on quality of frozen-thawed boar semen. Semen volume, sperm motility, sperm concentration, percentage of morphologically abnormal spermatozoa, total...

  • Influence of freeze-thawing on hyaluronic acid binding of human spermatozoa. Nijs, Martine; Creemers, Eva; Cox, Annemie; Janssen, Mia; Vanheusden, Elke; Castro-Sanchez, Yovanna; Thijs, Herbert; Ombelet, Willem // Reproductive BioMedicine Online (Reproductive Healthcare Limited;Aug2009, Vol. 19 Issue 2, p202 

    Mature human spermatozoa have at least three specific hyaluronic acid (HA) binding proteins present on their sperm membrane. These receptors play a role in the acrosome reaction, hyaluronidase activity, hyaluronan-mediated motility and sperm-zona and sperm-oolemmal binding. Cryopreservation of...

  • THE EFFECT OF CRYOPRESERVATION ON HUMAN SPERMATOZOAL DNA INTEGRITY. Gillott, David; Fragkogianni, Anastasia; Smith, Kathryn; Collins, Bonnie; Tozer, Amanda // Reproductive BioMedicine Online (Reproductive Healthcare Limited;Jun2010 S2 Supplement, Vol. 20, pS54 

    Aim: It has been suggested that cryopreservation may induce DNA damage in sperm. Whether this is due to necrosis, apoptosis or oxidative processes is questionable. Our aim was to investigate the effect of cryopreservation on spermatozoal DNA integrity and to establish an in house experimental...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics