TITLE

Genetic diversity among Campylobacter jejuni isolates from pets in Ireland

AUTHOR(S)
Acke, E.; McGill, K.; Lawlor, A.; Jones, B. R.; Fanning, S.; Whyte, P.
PUB. DATE
January 2010
SOURCE
Veterinary Record: Journal of the British Veterinary Association;1/23/2010, Vol. 166 Issue 4, p102
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Campylobacter jejuni isolates obtained from pets housed in shelters and in private households were subtyped by fla typing (using DdeI and HinfI restriction enzymes) and pulsed-field gel electrophoresis (PFGE) techniques. Composite fla cluster analysis on 78 C jejuni isolates was more discriminative than either single fla typing technique with 39.7 per cent single isolate patterns. PFGE on 52 C jejuni isolates revealed 53.8 per cent single isolate patterns and was the most discriminative method applied. A database of C jejuni subtyping profiles from pets in Ireland was assembled. The presence of genetic heterogeneity detected in the C jejuni subtypes suggests that pets can acquire the organisms from multiple potential sources. In addition, heterogeneity was detected in the C jejuni subtypes obtained by different culture methods within the same pet. There was a link between isolates from dogs in close contact in the same environment, confirming that this is a potential route of infection, and clusters were detected containing both cat and dog C jejuni isolates, suggesting possible interspecies transmission.
ACCESSION #
47911206

 

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