TITLE

Ethanol Production from Starch Hydrolyzates using Zymomonas mobilis and Glucoamylase Entrapped in Polyvinylalcohol Hydrogel

AUTHOR(S)
Martin Rebroš; Michal Rosenberg; Zuzana Grosová; L’udmila Krištofíková; Miroslav Paluch; Matej Šipöcz
PUB. DATE
September 2009
SOURCE
Applied Biochemistry & Biotechnology;Sep2009, Vol. 158 Issue 3, p561
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Abstract  The glucoamylase from Aspergillus niger, immobilized into poly(vinylalcohol) hydrogel lens-shaped capsules LentiKats®, was used for simultaneous saccharification and fermentation (SSF) with Zymomonas mobilis in free form. This system was stable in both the repeated batch and continuous mode of SSF. The microorganism was found to adsorb on the capsules with immobilized enzyme. This increased the ethanol productivity of the repeated batch system with 5% w/v of immobilized glucoamylase almost 2.1 times (7.2 g l−1 h−1) compared to free enzyme–free microorganism system (3.5 g l−1 h−1). The continuous SSF with the immobilized glucoamylase (11.5% w/v) tested for 15 days had productivity 10 g l−1 h−1, which is comparable to continuous experiments on semi-defined glucose medium (10 g l−1 h−1). These two systems were stable in both glucoamylase activity and microorganism productivity.
ACCESSION #
47868278

 

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