TITLE

SERS as tool for the analysis of DNA-chips in a microfluidic platform

AUTHOR(S)
Strelau, Katharina K.; Kretschmer, Robert; Möller, Robert; Fritzsche, Wolfgang; Popp, Jürgen
PUB. DATE
February 2010
SOURCE
Analytical & Bioanalytical Chemistry;Feb2010, Vol. 396 Issue 4, p1381
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
A sequence-specific detection method of DNA is presented combining a solid chip surface for immobilisation of capture DNAs with a microfluidic platform and a readout of the chip based on SERS. The solid chip surface is used for immobilisation of different capture DNAs, where target strands can be hybridised and unbound surfactants can be washed away. For the detection via SERS, short-labelled oligonucleotides are hybridised to the target strands. This technique is combined with a microfluidic platform that enables a fast and automated preparation process. By applying a chip format, the problems of sequence-specific DNA detection in solution phase by means of SERS can be overcome. With this setup, we are able to distinguish between different complementary and non-complementary target sequences in one sample solution.
ACCESSION #
47800049

 

Related Articles

  • Respirable antisense oligonucleotides: a new drug class for respiratory disease. Tanaka, Makoto; Nyce, Jonathan W. // Respiratory Research;2001, Vol. 2, p32 

    Respirable antisense oligonucleotides (RASONs), which attenuate specific disease-associated mRNAs, represent a new class of respiratory therapeutics with considerable potential. RASONs overcome previous obstacles that have impeded the development of antisense therapeutics targeting diseases in...

  • A high rate flow-focusing foam generator. Lorenceau, Elise; Sang, Yann Yip Cheung; Höhler, Reinhard; Cohen-Addad, Sylvie // Physics of Fluids;Sep2006, Vol. 18 Issue 9, p097103 

    We use a rigid axisymmetric microfluidic flow-focusing device to produce monodisperse bubbles, dispersed in a surfactant solution. The gas volume fraction of the dispersion collected out of this device can be as large as 90%, demonstrating that foam with solid-like viscoelastic properties can be...

  • Electrowetting --A versatile tool for controlling microdrop generation. Malloggi, F.; Gu, H.; Banpurkar, A. G.; Vanapalli, S. A.; Mugele, F. // European Physical Journal E -- Soft Matter;May/Jun2008, Vol. 26 Issue 1/2, p91 

    Integrating insulator-covered electrodes into a microfluidic flow focusing device (FFD) we demonstrate enhanced flexibility and control of the flow of two non-miscible liquids based on electrowetting (EW). In the parameters space, determined by liquid inlet pressures, we identify a specific...

  • Oliz, a suite of Perl scripts that assist in the design of microarrays using 50mer oligonucleotides from the 3' untranslated region. Hao Chen; Sharp, Burt M. // BMC Bioinformatics;2002, Vol. 3, p27 

    Background: Identifying reliable oligonucleotide sequences for use in microarray experiments is a complex process. Two key issues are the accuracy of the input sequences and the specificity of the oligonucleotide sequences. Results: We provide a suite of Perl scripts that facilitates the search...

  • Microarray results: how accurate are they? Kothapalli, Ravi; Yoder, Sean J.; Mane, Shrikant; Loughran Jr., Thomas P. // BMC Bioinformatics;2002, Vol. 3, p1 

    Background: DNA microarray technology is a powerful technique that was recently developed in order to analyze thousands of genes in a short time. Presently, microarrays, or chips, of the cDNA type and oligonucleotide type are available from several sources. The number of publications in this...

  • Direct microcontact printing of oligonucleotides for biochip applications. Thibault, C.; Le Berre, V.; Casimirius, S.; Trévisiol, E.; François, J.; Vieu, C. // Journal of Nanobiotechnology;2005, Vol. 3, p7 

    Background: A critical step in the fabrication of biochips is the controlled placement of probes molecules on solid surfaces. This is currently performed by sequential deposition of probes on a target surface with split or solid pins. In this article, we present a cost-effective procedure namely...

  • Surfactant modified deoxyribonucleic acid films: synthesis, interaction with acridine orange and luminescent properties. Kitazawa, Nobuaki; Aroonjaeng, W.; Aono, M.; Watanabe, Y. // Journal of Materials Science;Apr2011, Vol. 46 Issue 7, p2036 

    Dye-doped deoxyribonucleic acids (DNA)-tetradecyltrimethylammonium (TTA) films have been prepared. Acridine orange, known as a DNA-binding molecule, can be spontaneously doped by immersing the DNA-TTA film in an acetonitrile solution of the dye. Dye-doped samples exhibit two characteristic...

  • Low threshold amplified spontaneous emission from dye-doped DNA biopolymer. Hung, Yu-Chueh; Su, Che-Hsuan; Huang, Hsien-Wen // Journal of Applied Physics;Jun2012, Vol. 111 Issue 11, p113107 

    In this study, we investigate the amplified spontaneous emission (ASE) properties and conduct a comparative study for two kinds of dye-doped deoxyribonucleic acid (DNA) biopolymers. The system consists of optical films made of DNA modified by two types of surfactants and doped with a common...

  • Irreversible nanogel formation in surfactant solutions by microporous flow. Vasudevan, Mukund; Buse, Eric; Lu, Donglai; Krishna, Hare; Kalyanaraman, Ramki; Shen, Amy Q.; Khomami, Bamin; Sureshkumar, Radhakrishna // Nature Materials;May2010, Vol. 9 Issue 5, p436 

    Self-assembly of surfactant molecules into micelles of various shapes and forms has been extensively used to synthesize soft nanomaterials. Translucent solutions containing rod-like surfactant micelles can self-organize under flow to form viscoelastic gels. This flow-induced structure (FIS)...

Share

Read the Article

Courtesy of THE LIBRARY OF VIRGINIA

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics