TITLE

Quartz crystal microbalance, a valuable tool for elucidation of interactions between apoB-100 peptides and extracellular matrix components

AUTHOR(S)
D'Ulivo, Lucia; Saint-Guirons, Julien; Ingemarsson, Björn; Riekkola, Marja-Liisa
PUB. DATE
February 2010
SOURCE
Analytical & Bioanalytical Chemistry;Feb2010, Vol. 396 Issue 4, p1373
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Atherosclerosis has received wide attention as a primary cause of premature death in developed countries. The retention of low-density lipoprotein (LDL) particles in the intima, the inner layer of the capillaries, has been imputed as the main cause of the development of atherosclerotic plaques. The entrapment of LDL is mainly due to the specific interaction between the lysine-rich site on apolipoprotein B-100 (apoB-100), a major apolipoprotein of LDL, and extracellular matrix (ECM) components such as collagen, proteoglycans, and glycosaminoglycans (GAGs). Although valuable techniques already exist for studies on apoB-100 and ECM interactions, there is continued need for miniaturized tools that can complement the tools already available and even provide totally new data. This work explores the applicability of the quartz crystal microbalance (QCM) for interaction studies between apoB-100 peptide fragments and various components of the ECM. Two positive peptide fragments, PP and PP2, and two components of the ECM, collagen I and a selected GAG, chondroitin 6-sulfate (C6S), were immobilized on polystyrene and carboxyl sensor chips. C6S was injected as analyte for PP- and PP2-coated surfaces, while PP was the analyte for collagen I and C6S surfaces. The estimated dissociation constant ( KD) indicates that the interactions occur via the positive residues, lysine and arginine, of apoB-100. The continuous-flow QCM system employed in this study is shown to be an excellent tool for the elucidation of interactions between these types of biomolecules. [Figure not available: see fulltext.]
ACCESSION #
47800030

 

Related Articles

  • Similarities and differences in structure, expression, and functions of VLDLR and ApoER2. Reddy, Sunil S.; Connor, Teal E.; Weeber, Edwin J.; Rebeck, William // Molecular Neurodegeneration;2011, Vol. 6 Issue 1, p30 

    Very Low Density Lipoprotein Receptor (VLDLR) and Apolipoprotein E Receptor 2 (ApoER2) are important receptors in the brain for mediating the signaling effects of the extracellular matrix protein Reelin, affecting neuronal function in development and in the adult brain. VLDLR and ApoER2 are...

  • Preparation and investigation of 99m technetium-labeled low-density lipoproteins in rabbits with experimentally induced hypercholesterolemia. Bozóky, Z.; Balogh, L.; Máthé, D.; Fülöp, L.; Bertók, L.; Jánoki, Gy. A. // European Biophysics Journal;2004, Vol. 33 Issue 2, p140 

    Low-density lipoproteins (LDL) were radiolabeled in atherosclerosis studies. The aim was to investigate the biodistribution and uptake of [sup 99m]Tc-labeled LDL by atherosclerotic plaques in experimentally induced hyperlipidemia. Rabbits were fed a diet containing 2% cholesterol for 60 days to...

  • Lipids, Lipoproteins, Apolipoproteins, and Atherosclerosis. Kreisberg, Robert A. // Annals of Internal Medicine;Nov83, Vol. 99 Issue 5, p713 

    Editorial. Examines the role of lipids, lipoproteins and apolipoproteins in the development of atherosclerosis. Mechanism of apoprotein A; Importance of apoprotein E in chylomicrons and in very-low-density lipoproteins; Presence of normal low-density lipoprotein in patients with coronary...

  • Subendothelial retention of atherogenic lipoproteins in early atherosclerosis. Skalen, Kristina; Gustafsson, Maria; Rydberg, Ellen Knutsen; Hulten, Lilemor Mattson; Wiklund, Olov; Innerarity, Thomas L.; Boren, Jan // Nature;6/13/2002, Vol. 417 Issue 6890, p750 

    Presents a study that examined the role of the subendothelial retention of atherogenic apolipoprotein B-containing low-density lipoproteins (LDL) in early atheroscelrosis in transgenic mice. Effect of an atherogenic diet on the aorta of transgenic mice; Analysis of the uptake and degradation of...

  • A truncated splice variant of LRP1. Kolb, M.; Trettner, S.; Büttner, S.; Engel, K.; Seiboth, T.; Huse, K.; Birkenmeier, G. // Proceedings of the Physiological Society;2013, p332P 

    The low-density lipoprotein receptor-related protein-1 (LRP1), identical to the Alpha2-macroglobulin receptor (A2MR), is a member of the LDL receptor superfamily. The ubiquitously expressed LRP1 in particular is a scavenger receptor that mediates clearance of over 50 ligands from the...

  • Decreased clearance of low-density lipoprotein in uremic patients under dialysis treatment. Hörkkö, Sohvi; Huttunen, Kaisa; Kesäniemi, Y. Antero // Kidney International;Jun1995, Vol. 47 Issue 6, p1732 

    The clearance of low-density lipoprotein (LDL) was studied in eleven patients on hemodialysis (HD) treatment and nine patients on continuous ambulatory peritoneal dialysis (CAPD) treatment and compared with the clearance of LDL in nine control subjects. The clearance rates for LDL (fractional...

  • Decreased longevity in Japanese men, associated with low-molecular-weight apolipoprotein(a) phenotypes. Matsubara, Miyao; Akita, Harukuni; Shibuya, Hitoshi; Chiba, Hitoshi // Annals of Clinical Biochemistry;Mar2001, Vol. 38 Issue 2, p120 

    Lipoprotein(a) [Lp(a)] is an established risk factor for atherosclerosis. High plasma Lp(a) concentrations are associated with low-molecular-weight (LMW) apolipoprotein(a) (apo a) phenotypes, which raises the question of whether LMW apo a phenotypes occur less frequently in the elderly. To...

  • The Apolipoprotein-AI Mimetic Peptide L4F at a Modest Dose Does Not Attenuate Weight Gain, Inflammation, or Atherosclerosis in LDLR-Null Mice. Averill, Michelle M.; Kim, Eung Ju; Goodspeed, Leela; Wang, Shari; Subramanian, Savitha; Den Hartigh, Laura J.; Tang, Chongren; Ding, Yilei; Reardon, Catherine A.; Getz, Godfrey S.; Chait, Alan // PLoS ONE;Oct2014, Vol. 9 Issue 10, p1 

    Objective: High density lipoprotein (HDL) cholesterol levels are inversely related to cardiovascular disease risk and associated with a reduced risk of type 2 diabetes. Apolipoprotein A-I (apoA-I; major HDL protein) mimetics have been reported to reduce atherosclerosis and decrease adiposity....

  • Human Apolipoprotein A-I-Derived Amyloid: Its Association with Atherosclerosis. Ramella, Nahuel A.; Rimoldi, Omar J.; Prieto, Eduardo D.; Schinella, Guillermo R.; Sanchez, Susana A.; Jaureguiberry, María S.; Vela, María E.; Ferreira, Sergio T.; Tricerri, M. Alejandra // PLoS ONE;2011, Vol. 6 Issue 7, p1 

    Amyloidoses constitute a group of diseases in which soluble proteins aggregate and deposit extracellularly in tissues. Nonhereditary apolipoprotein A-I (apoA-I) amyloid is characterized by deposits of nonvariant protein in atherosclerotic arteries. Despite being common, little is known about the...

Share

Read the Article

Courtesy of VIRGINIA BEACH PUBLIC LIBRARY AND SYSTEM

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics