Determination of hepatitis C virus genotypes among blood donors in Ahvaz, Iran

Farshadpour, F.; Makvandi, M.; Samarbafzadeh, A. R.; Jalalifar, M. A.
January 2010
Indian Journal of Medical Microbiology;Jan2010, Vol. 28 Issue 1, p54
Academic Journal
This study aims to determine the genotypes of hepatitis C virus (HCV) among blood donors at Ahvaz Blood Transfusion Centre. Blood samples were taken from 2376 blood donors - 1795 (75.54%) male and 581(24.45%) female - who referred to Ahvaz Blood Transfusion Centre during 2007-2008. Detection of anti-HCV antibody for all the donors was carried out by ELISA and the confirmatory RIBA tests. HCV RT-PCR followed by RFLP test was carried out for anti-HCV positive samples. Out of 2376 blood donors, only 55 (2.3%) male donors showed to be positive for HCV antibody by ELISA and RIBA tests out of which 45(1.8%) donors were positive for RT-PCR test. Female donors were negative for HCV antibody. The result of HCV genotyping by RFLP test showed 24 (53.3%) for 1a, 17 (37.7%) for 3a (α) and 4 (8.8%) for 3a (β) genotypes respectively. In conclusion, high prevalence of 53.3% HCV 1a genotype was observed among blood donors in Ahvaz city.


Related Articles

  • Detection of HCV antibody among intending blood donors. Okonko, I. O.; Oyediji, T. O.; Anugweje, K. C.; Adeniji, F. O.; Alli, J. A.; Abraham, O. A. // Nature & Science;Jan2012, Vol. 10 Issue 1, p53 

    This study was carried out to detect hepatitis C virus (HCV) antibody among intending blood donors. In order to estimate the prevalence rate of HCV antibody, blood samples were screened by parallel diagnostic method using One Step Strip Style HCV test kits, by Dia Spot® Diagnostics, USA and...

  • Seroprevalence of anti-HCV antibodies among blood donors of north India. Makroo, R. N.; Singh Walia, Rimpreet; Chowdhry, Mohit; Bhatia, Aakanksha; Hegde, Vikas; Rosamma, N. L. // Indian Journal of Medical Research;Jul2013, Vol. 138 Issue 1, p125 

    Background & objectives: Transfusion of blood and blood products although considered as a life saving treatment modality, but may lead to certain infectious and non-infectious complications in the recipients. The purpose of this analysis was to monitor the seroprevalence of anti-HCV antibody in...

  • Incidence of non-A, non-B hepatitis after screening blood donors for antibodies to hepatitis C virus and surrogate markers. Barrera, Josep M.; Bruguera, Miquel; Ercilla, Guadalupe; Sanchez-Tapias, Jose M.; Gil, Maria P.; Gil, Cristina; Costa, Josep; Gelabert, Antonio; Rodes, Joan; Castillo, Ricardo; Barrera, J M; Bruguera, M; Ercilla, M G; Sánchez-Tapias, J M; Gil, M P; Costa, J; Gelabert, A; Rodés, J; Castillo, R // Annals of Internal Medicine;10/15/91, Vol. 115 Issue 8, p596 

    Objective: To compare the effect of screening blood donors for antibodies to hepatitis C virus (anti-HCV) on the incidence of non-A, non-B hepatitis in recipients with that of screening blood donors for antibodies to hepatitis B core antigen (anti-HBc) and elevated alanine...

  • Frequencies of HBV, HCV, HIV, and Syphilis Markers Among Blood Donors: A Hospital-Based Study in Hodeidah, Yemen. Mohammed Saghir, Sultan Ayesh; Al-Hassan, Faisal Muti; Ali Alsalahi, Omar Saeed; Abdul-Alaziz, Abdullah Ebrahim; Baqir, Huda Salman // Tropical Journal of Pharmaceutical Research;Feb2012, Vol. 11 Issue 1, p132 

    Purpose: This study aimed to determine the frequency rates of human immunodeficiency virus (HIV), hepatitis B virus (HBV), hepatitis C virus (HCV), and syphilis among blood donors. Methods: Physically fit persons aged 18 - 48 years who came for blood donation at the blood bank unit of the...

  • Kan bağışçılarında HBsAg, anti-HCV, anti-HIV ve VDRL testi sonuçlarının deÄŸerlendirilmesi. Deveci, Özcan; Tekin, Alicem; Günbay, Seda Sibel; Kılıç, Dilek; Kaygusuz, Sedat; Ağalar, Canan; Özer, Türkan Toka // Journal of Clinical & Experimental Investigations / Klinik ve De;2011, Vol. 2 Issue 4, p416 

    Objectives: The most frequently encountered complication in the transfusion of blood and blood products are transmitted infections from these products. Infections caused by hepatitis B virus (HBV), hepatitis C virus (HCV), and human immunodeficiency virus (HIV) remain the leading most important...

  • High rate of infectivity and liver disease in blood donors with antibodies to hepatitis C virus. Esteban, Juan Ignacio; Lopez-Talavera, Juan Carlos; Genesca, Juan; Madoz, Pedro; Viladomiu, Luis; Muniz, Eduardo; Martin-Vega, Camen; Rosell, Manuel; Allende, Helena; Vidal, Xavier; Gonzalez, Antonio; Hernandez, Jose Manuel; Esteban, Rafael; Guardia, Jaime; Esteban, J I; López-Talavera, J C; Genescà, J; Madoz, P; Viladomiu, L; Muñiz, E // Annals of Internal Medicine;9/15/91, Vol. 115 Issue 6, p443 

    Objective: To determine the epidemiologic, clinical, serologic, and histologic importance of antibodies to hepatitis C virus (anti-HCV) in blood donors.Design: Cross-sectional identification and prospective evaluation of seropositive donors; retrospective assessment of...

  • Prevalence of hepatitis C virus antibodies and genotypes in asymptomatic, first-time blood donors in Namibia. Vardas, E.; Sitas, F.; Seidel, K.; Casteling, A.; Sim, J. // Bulletin of the World Health Organization;1999, Vol. 77 Issue 12, p965 

    Determines the prevalence of hepatitis C virus (HCV) antibodies and genotypes in Namibia. Increase in HCV positivity with increasing age; Importance of screening blood donors for HCV; Prevention of HCV transmission.

  • Risk factors for hepatitis C virus infection among blood donors in southern Brazil: a case-control study. Brandão, Ajacio B. M.; Fuchs, Sandra Costa // BMC Gastroenterology;2002, Vol. 2, p18 

    Background: In Brazil, it is estimated that between 2.5 and 4.9% of the general population present anti-hepatitis C virus (HCV) antibodies, which corresponds to as many as 3.9 to 7.6 million chronic carriers. Chronic liver disease is associated with HCV infection in 20% to 58% of the Brazilian...

  • THE USEFULNESS OF UMELOSA HCV CUALITATIVO KIT AS SUPPLEMENTAL TEST FOR CONFIRMATION OF HCV INFECTION. Gonzalez-Perez, I.; González, Y. J.; Vina-Rodriguez, A.; Armas, A.; Solís, R. L. // Revista VacciMonitor (Vacunología y Temas Afines);Oct2002, Vol. 11 Issue 4, p1 

    Screened population: Forty voluntary blood donors from two different blood banks in Havana, Cuba, who were repeatedly reactive on the routine screening of antibodies to hepatitis C virus, by UMELISA HCV test, were analysed for the presence of HCV RNA, employing a nested PCR assay UMELOSA HCV...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics