Spectroscopic characterization of bionanoparticles originating from newly developed self-forming synthetic PEGylated lipids (QuSomes)

Bista, Rajan K.; Bruch, Reinhard F.; Covington, Aaron M.
February 2010
Analytical & Bioanalytical Chemistry;Feb2010, Vol. 396 Issue 3, p1045
Academic Journal
In this work, we have aimed to merge the advantages of nanotechnology and biophotonics in conjunction with vibrational spectroscopic techniques in order to understand the various aspects of new kinds of synthetic bionanoparticles originating from self-forming synthetic biopolymers known as polyethylene glycol (PEG)ylated lipids. In particular, two complementary molecular spectroscopic techniques based on thin-layered Fourier transform infrared and confocal laser tweezers. Raman spectroscopy has been employed for the investigations of newly developed artificial PEGylated lipids trademarked as QuSomes. These novel types of synthetic lipids are composed of 1,2-dimyristoyl- rac-glycerol-3-dodecaethylene glycol (GDM-12), 1,2-dioleoyl- rac-glycerol-3-dodecaethylene glycol (GDO-12), and 1,2-distearoyl- rac-glycerol-3-triicosaethylene glycol (GDS-23). The lipid labeled GDM-12 has saturated 14 acyl chains whereas GDO-12 is characterized by monounsaturated 18 acyl chains, and GDS-23 is composed of saturated 18 acyl chains in their hydrophobic chain. Similarly, GDM-12 and GDO-12 contain 12 units, and GDS-23 contains 23 units of hydrophilic PEG head groups. In contrast to conventional phospholipids, this novel kind of lipid can form liposomes spontaneously upon hydration, without the input of external activation energy. In addition, fluorescence correlation spectroscopy has been utilized to measure the size distribution of such nanoparticles in suspension as well as scanning electron microscopy has been applied for the imaging purposes. Although such PEGylated lipids show a common spectral pattern, important differences in the spectra have been observed, enabling us to distinguish these different lipids on the basis of characteristic features calculated from the spectroscopic band component analysis. Finally, in this study, detailed spectroscopic results due to the vibrational band assignments and band component analysis corresponding to various functional groups for individual nanoparticles have been analyzed and discussed.


Related Articles

  • Single Step Synthesis of Amino-Functionalized Magnetic Nanoparticles with Polyol Technique at Low Temperature. Songvorawit, Nut; Tuitemwong, Kooranee; Tuitemwong, Pravate // ISRN Nanotechnology;2011, Special section p1 

    The synthesis and characterization of amino-functionalized magnetic nanoparticles (amino-MNPs) were carried out. Amino-MNPs were prepared using polyol technique in an autoclave (121°C) without inert gas. The characterization of particles employed conventional SEM-EDS, TEM, XRD, FTIR, and VSM...

  • Preparation and Characterization of Polyethylene Glycol Coated Silica Nanoparticles for Drug Delivery Application. Thangaraja, A.; Savitha, V.; Jegatheesan, K. // International Journal of Nanotechnology & Applications;2010, Vol. 4 Issue 1, p31 

    Polyethylene Glycol (PEG) coated silica nanoparticles were successfully synthesized and were used to load the drug Ibuprofen. The silica nanoparticles synthesized through Stober's method were in the range of 10 - 150 nm in dimension. Monodisperse, spherical PEG -coated silica nanoparticles have...

  • A novel method for the preparation of silver/chitosan- O-methoxy polyethylene glycol core shell nanoparticles. Abdel-Mohsen, A.; Aly, A.; Hrdina, R.; El-Aref, Ahmed // Journal of Polymers & the Environment;Jun2012, Vol. 20 Issue 2, p459 

    Uniformly sized silver/chitosan- O-methoxy polyethylene glycol (chitosan- O-MPEG) core shell nanoparticles with different degree of substitution were synthesized. Thus, N-phthaloyl chitosan is reacted with polyethylene glycol monomethyl ether iodide in the presence of silver oxide by the...

  • Erratum to: Polyethylene glycol functionalized gold nanoparticles: the influence of capping density on stability in various media. Manson, Joanne; Kumar, Dhiraj; Meenan, Brian J.; Dixon, Dorian // Gold Bulletin;2011, Vol. 44 Issue 3, p195 

    A correction to the article "Polyethylene glycol functionalized gold nanoparticles: the influence of capping density on stability in various media" that was published in the August 3, 2011 issue is presented.

  • Study of Electro-Optical Properties of ZnS Nanoparticles Prepared by Colloidal Particles Method. Rahdar, A.; Arbabi, V.; Ghanbari, H. // World Academy of Science, Engineering & Technology;2012, Issue 61, p657 

    ZnS nanoparticles of different size have been synthesized using a colloidal particles method. Zns nanoparticles prepared with capping agent (mercaptoethanol) then were characterized using X-ray diffraction (XRD) and UV-Vis spectroscopy. The particle size of the nanoparticles calculated from the...

  • The Formation and Binding of Gold Nanoparticles onto Wool Fibres. Johnston, James H.; Burridge, Kerstin A.; Kelly, Fern M. // AIP Conference Proceedings;7/23/2009, Vol. 1151 Issue 1, p189 

    This paper presents the novel use of nanosize gold with different plasmon resonance colours, as stable colourfast colourants on wool fibres for use in high quality fabrics and textiles. The gold nanoparticles are synthesised by the controlled reduction of Au3+ in the AuCl4- complex to Au0 onto...

  • A general thermal process for the one-step preparation of well-stable noble metal nanoparticles. Yonglan Luo // Colloid Journal;Sep2008, Vol. 70 Issue 5, p669 

    Heating sodium 3-thiophenemalonic acid-noble metal salt aqueous solution at 100°C, without the extra step of introducing other reducing agents and protective agents, results in the formation of well-stable noble metal that includes Au, Ag, Pt, or Pd nanoparticles. As-formed colloidal...

  • Structure of polyethylene films containing copper nanoparticles. Éfendiev, É. G.; Gadzhieva, N. N.; Il'yasly, T. M.; Abbasova, R. F.; Yakh'yaev, F. F. // Journal of Applied Spectroscopy;May2006, Vol. 73 Issue 3, p462 

    We have used IR spectroscopy to study the effect of Cu nanoparticles on the degree of crystallinity in nanostructured high-pressure polyethylene (PE) films. We have shown that for ∼1 mass % Cu in the polyethylene films, we observe the maximum degree of crystallinity. This is connected with...

  • FORMATION OF SILVER NANOPARTICLES BY MICROWAVE IRRADIATION. ŠiIieikaite, A.; Prosyčevas, I.; Puišo, J.; Juraitis, A.; Guobienė, A. // International Conference: Radiation Interaction with Material & ;2006, p94 

    In the present research silver colloid was produced by affecting solution with microwave radiation. Formation of silver nanoparticles has been confirmed by UV- VIS measurements. The surface plasmon resonance peak in absorption spectra of silver particles showed an absorption maximum at 440 mm...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics