Use of the quartz crystal microbalance to monitor ligand-induced conformational rearrangements in HIV-1 envelope protein gp120

Hyun-Su Lee; Contarino, Mark; Umashankara, M.; Schön, Arne; Freire, Ernesto; Smith III, Amos B.; Chaiken, Irwin M.; Penn, Lynn S.
February 2010
Analytical & Bioanalytical Chemistry;Feb2010, Vol. 396 Issue 3, p1143
Academic Journal
We evaluated the potential of a quartz crystal microbalance with dissipation monitoring (QCM-D) to provide a sensitive, label-free method for detecting the conformational rearrangement of glycoprotein gp120 upon binding to different ligands. This glycoprotein is normally found on the envelope of the HIV-1 virus and is involved in viral entry into host cells. It was immobilized on the surface of the sensing element of the QCM-D and was exposed to individual solutions of several different small-molecule inhibitors as well as to a solution of a soluble form of the host cell receptor to which gp120 binds. Instrument responses to ligand-triggered changes were in qualitative agreement with conformational changes as suggested by other biophysical methods. [Figure not available: see fulltext.]


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