TITLE

Methotrexate released in vitro from bone cement inhibits human stem cell proliferation in S/G2 phase

AUTHOR(S)
Egon Prochazka; Tomas Soukup; Milos Hroch; Leos Fuksa; Eva Brcakova; Jolana Cermanova; Gabriela Kolouchova; Karel Urban; Jaroslav Mokry; Stanislav Micuda
PUB. DATE
January 2010
SOURCE
International Orthopaedics;Jan2010, Vol. 34 Issue 1, p137
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Abstract  Methotrexate (MTX) released from bone cement showed a useful local effect in animal models of bone tumours. However, local toxic reactions such as impaired wound healing were observed in areas surrounding the MTX-loaded implant. Therefore, we hypothesised that MTX released from bone cement would have harmful effects on human mesenchymal stem cells (MSC)—one of the basic components of bone marrow and tissue reparatory processes. Moreover, elution of MTX was calculated from implants prepared either with liquid or powdered MTX. During the 28-day incubation, the cement compounded with liquid MTX showed the highest elution rate of the drug. MTX released from pellets produced a significant decrease in proliferation of MSC as a consequence of a blockade of their cell cycle in the S/G2 phase. These findings indicate impairment of stem cell function in marginal areas surrounding the MTX-loaded cement and may help to explain problems with regeneration of tissues in these locations.
ACCESSION #
47380012

 

Related Articles

  • Curcumin and Bioperine Target Breast Cancer Stem Cells.  // Art of Healing;Jun-Aug2010, Vol. 2 Issue 31, p13 

    The article focuses on the findings of the research conducted by the University of Michigan researchers which shows that curcumin and bioperine concentration helps to lessen the stem cells growth that stimulate cancer of the breast.

  • ERBB3 Positively Correlates with Intestinal Stem Cell Markers but Marks a Distinct Non Proliferative Cell Population in Colorectal Cancer. Jardé, Thierry; Kass, Lisa; Staples, Margaret; Lescesen, Helen; Carne, Peter; Oliva, Karen; McMurrick, Paul J.; Abud, Helen E. // PLoS ONE;9/14/2015, Vol. 10 Issue 9, p1 

    Several studies have suggested ERBB3/HER3 may be a useful prognostic marker for colorectal cancer. Tumours with an intestinal stem cell signature have also been shown to be more aggressive. Here, we investigate whether ERBB3 is associated with intestinal stem cell markers in colorectal cancer...

  • Local recurrences after curettage and cementing in long bone giant cell tumor. Saikia, Kabul C.; Bhattacharyya, Tulsi D.; Bhuyan, Sanjeev K.; Bordoloi, Bikas; Durgia, Bharat; Ahmed, Firoz // Indian Journal of Orthopaedics;Mar/Apr2011, Vol. 45 Issue 2, p168 

    Background: Giant cell tumor of bone (GCT) is a benign lesion with great propensity for local recurrence. This study aimed to analyse the rates of local recurrence and its possible predisposing factors in Campanacci's Grade III and II GCT of long bones following intralesional curettage and bone...

  • Kynurenic acid inhibits colon cancer proliferation in vitro: effects on signaling pathways. Walczak, Katarzyna; Turski, Waldemar; Rajtar, Grażyna // Amino Acids;Oct2014, Vol. 46 Issue 10, p2393 

    Kynurenic acid (KYNA), a tryptophan metabolite, inhibits proliferation of several cancer cell lines including colon cancer, renal cancer and glioblastoma cells. Previous studies reported that inhibitory properties of KYNA may be related to interactions of KYNA with cell cycle regulators and...

  • Georgia scientists create pigs which may hold the key to developing stem cells to treat human illnesses.  // Transplant News;Jun2010, Vol. 20 Issue 6, p8 

    The article reports that scientists from the University of Georgia have produced pigs which may aid in treating human diseases through stem cells.

  • Evolving technology: creating kidney organoids from stem cells. Chambers, Joseph M.; McKee, Robert A.; Drummond, Bridgette E.; Wingert, Rebecca A. // AIMS Bioengineering;2016, Vol. 3 Issue 3, p305 

    The kidney is a complex organ whose excretory and regulatory functions are vital for maintaining homeostasis. Previous techniques used to study the kidney, including various animal models and 2D cell culture systems to investigate the mechanisms of renal development and regeneration have many...

  • TLR7 inhibition. Eigenbrod, Tatjana; Dalpke, Alexander H. // JAK-STAT;Jan-Mar2013, Vol. 2 Issue 1, p1 

    The article discusses the findings of a study which describes the role of Toll-like receptors 7 (TLR7) in the development and progression of pancreatic cancer. The researchers have demonstrated the inhibition of TLR7 in cell cyle regulation and activation of multiple cell signaling pathways...

  • Loss of Rassf1a enhances p53-mediated tumor predisposition and accelerates progression to aneuploidy. Tommasi, S; Besaratinia, A; Wilczynski, S P; Pfeifer, G P // Oncogene;2/10/2011, Vol. 30 Issue 6, p690 

    Loss of RASSF1A leads to several mitotic abnormalities, including cytokinesis failure and tetraploidization. Uncontrolled proliferation of tetraploid cells is known to trigger genomic instability and tumor development and is normally prevented through activation of a p53-dependent tetraploidy...

  • Geminin Is Required for the Maintenance of Pluripotency. Tabrizi, Golnaz A.; Böse, Kerstin; Reimann, Yvonne; Kessel, Michael // PLoS ONE;Sep2013, Vol. 8 Issue 9, p1 

    Pluripotency requires the expression of the three core transcriptions factors Oct4, Sox2 and Nanog, as well as further, complementary proteins. The geminin protein is part of this network, and was shown to play a role in the regulation of DNA replication, the control of the cell cycle, and the...

Share

Read the Article

Courtesy of VIRGINIA BEACH PUBLIC LIBRARY AND SYSTEM

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics