Ultrafiltration and Diafiltration

Houp, Rachel C.; Fetterolf, David M.
November 2009
Journal of Validation Technology;Autumn2009, Vol. 15 Issue 4, p40
Academic Journal
The article discusses the basic functions and mechanisms of ultrafiltration (UF) and diafiltration (DF), which are used as downstream processing steps for product concentration. UF refers to a biotech process for concentration a dilute product stream, while DF is used to exchange product into a desired buffer. In biotechnology, DF is also used to prepare the product stream for a chromatography step or to exchange drug product into the final formulation buffer. A flat plate cassette is the most common UF/DF configuration that is used for processing of biological products.


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