TITLE

Six-shogaol Inhibits Production of Tumour Necrosis Factor Alpha, Interleukin-1 Beta and Nitric Oxide from Lipopolysaccharide-stimulated RAW 264.7 Macrophages

AUTHOR(S)
Levy, A. S. A.; Simon, O. R.
PUB. DATE
September 2009
SOURCE
West Indian Medical Journal;Sep2009, Vol. 58 Issue 4, p295
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Objective: We previously reported that 6-shogaol, a phenolic compound from ginger, has antiinflammatory properties in a Complete Freund's Adjuvant (CFA) model of mono-arthritic rats. In the present study, we investigated the effects of 6-shogaol on the production of inflammatory mediators from lipopolysaccharide (LPS) activated RAW 264.7 macrophages. These mediators (TNF-α, IL-1-β and NO) and their output from macrophages are involved in various pathophysiological events of chronic inflammation and arthritis. Methods: Effects of 6-shogaol were investigated on the production of the mediators TNF-α, IL-1-β and NO (measured as nitrate) from macrophages. Lipopolysaccharide activated RAW 264.7 macrophages were cultured in the presence and absence of 6-shogaol (2μM, 10μM and 20μM) and ELISA was used to quantify the output of the mediators. Results: 6-shogoal (2μM, 10μM and 20μM) significantly inhibited the production of nitric oxide (NO), IL-1β and TNF-α from the LPS activated RAW264.7 macrophages. Conclusion: The results suggest that macrophages are targets for the anti-inflammatory effects of 6-shogaol. Also, the inhibitory effects against TNF-α, IL-1β and NO production from LPS activated macrophages are cellular mechanisms by which 6-shogaol produced its anti-inflammatory effects. These mechanisms provide an explanation of the protection by 6-shogaol against development of joint inflammation and cartilage degradation in CFA induced mono-arthritis that we previously demonstrated (1). Based on these results with 6-shogaol, there is evidence that it exhibits exploitable anti-inflammatory properties.
ACCESSION #
47333548

 

Related Articles

  • Influence of different anaesthetics on pro-inflammatory cytokine expression in rat spleen. M. Bette; S. Schlimme; R. Mutters; S. Menendez; S. Hoffmann; S. Schulz // Laboratory Animals;Jul2004, Vol. 38 Issue 3, p272 

    We examined the effect of five anaesthetic drugs commonly used in laboratory animal research (tribromoethanol, ketamine/xylazine, chloral hydrate, pentobarbital, and urethane) on the expression of four pro-inflammatory cytokines. The anaesthetic agents were applied at dosages normally used for...

  • EFFECTS OF DIFFERENT ANESTHETIC AGENTS ON GM-CSF, MCP1, IL1α AND TNFα LEVELS IN RAT SEPSIS MODEL. VOJVODIC, D.; MILJANOVIC, OLIVERA; DJURDJEVIC, D.; GATARIC, S.; STANOJEVIC, I.; OBRADOVIC, DRAGANA; SURBATOVIC, MAJA; FRANCUSKI, JELENA // Acta Veterinaria;Mar2013, Vol. 63 Issue 2/3, p125 

    Anesthetic agents could alter the course and outcome of physical trauma, as well as experimentally or naturally occurring severe infections, by regulating several immune response mechanisms. The aim of our study was to investigate the influence of several commercially used anesthetic agents...

  • Anti-inflammatory Effects of Magnolia sieboldii Extract in Lipopolysaccharide-Stimulated RAW264.7 Macrophages. Oyungerel, Baatartsogt; Lim, Heekyong; Chi-Ho Lee; Eun-Hee Choi; Guan-Hao Li; Kang-Duk Choi // Tropical Journal of Pharmaceutical Research;Dec2013, Vol. 12 Issue 6, p913 

    Purpose: To investigate the effect of Magnolia sieboldii extract (MSE) on the production of proinflammatory cytokines by macrophage. Methods: The whole plant of M. sieboldii was extracted with methanol at room temperature. The in vitro anti-inflammatory activity of MSE was investigated on...

  • Interleukin-1 beta guides the migration of cortical neurons. Lei Ma; Xiao-wei Li; Shi-jun Zhang; Feng Yang; Ge-min Zhu; Xiao-bing Yuan; Wen Jiang // Journal of Neuroinflammation;2014, Vol. 11 Issue 1, p1 

    Background Proinflammatory cytokine interleukin-1beta (IL-1β) is expressed at high levels in the developing brain and declines to low constitutive levels in the adult. However, the pathophysiological function of IL-1β during brain development remains elusive. In this study, we investigated...

  • The Dose-Dependent Immunoregulatory Effects of the Nitric Oxide Synthase Inhibitor NG-Nitro-L-Arginine Methyl Ester in Rats with Sub-Acute Peritonitis. Chien-Chou Hsiao; Chien-Hsing Lee; Lon-Yen Tsao; Hui-Chen Lo; Caldwell, Charles C. // PLoS ONE;Aug2012, Vol. 7 Issue 8, Special section p1 

    Background:Chronic inflammation accompanied by arginine deficiency, immune dysfunction, and excess nitric oxide (NO) production is a clinical condition found in patients with peritonitis. A previous study showed that the nonselective NOS inhibitor NG-nitro-L-arginine methyl ester (L-NAME) ...

  • Pheophytin a Inhibits Inflammation via Suppression of LPS-Induced Nitric Oxide Synthase-2, Prostaglandin E2, and Interleukin-1β of Macrophages. Chun-Yu Lin; Chien-Hsing Lee; Yu-Wei Chang; Hui-Min Wang; Chung-Yi Chen; Yen-Hsu Chen // International Journal of Molecular Sciences;2014, Vol. 15 Issue 12, p22819 

    Inflammation is a serious health issue worldwide that induces many diseases such as sepsis. There has been a vast search for potentially effective drugs to decrease mortality from sepsis. Pheophytin a is a chlorophyll-related compound derived from green tea. We found that pre-treatment with...

  • The Role of Nitric Oxide and the Unfolded Protein Response in Cytokine-Induced ß-Cell Death. Chambers, Kari T.; Unverferth, Julie A.; Weber, Sarah M.; Wek, Ronald C.; Urano, Fumihiko; Corbett, John A. // Diabetes;Jan2008, Vol. 57 Issue 1, p124 

    OBJECTIVE--The unfolded protein response (UPR) is a conserved cellular response designed to alleviate damage and promote survival of cells experiencing stress; however, prolonged UPR activation can result in apoptotic cell death. The UPR, activated by cytokine-induced nitric oxide (NO)...

  • Brain microvascular pericytes are immunoactive in culture: cytokine, chemokine, nitric oxide, and LRP-1 expression in response to lipopolysaccharide.  // Journal of Neuroinflammation;2011, Vol. 8 Issue 1, p139 

    The article investigates the production of immune mediators such as nitric oxide (NO), cytokines, and chemokines in rats to find the immunological profile of brain pericytes in culture in the quiescent and immune-challenged state. The effects of immune challenge on pericyte expression of low...

  • Experimental model of peritoneal adhesion formation. S. Skal’skiy; G. Shamray; T. Dolgich; B. Reys; T. Sokolova; A. Kotchetov; T. Priticina // Bulletin of Experimental Biology & Medicine;Oct2007, Vol. 144 Issue 4, p588 

    Abstract  Experimental model of peritoneal adhesion formation developing in rats and rabbits as a result of dosed autohemoperitoneum is described. The role of peritoneal macrophages, their microenvironment cells, and cytokines in the development of peritoneal adhesions is demonstrated.

Share

Read the Article

Courtesy of VIRGINIA BEACH PUBLIC LIBRARY AND SYSTEM

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics