TITLE

Amifostine (WR2721) Confers DNA Protection to in vivo Cisplatin-Treated Murine Peripheral Blood Leukocytes

AUTHOR(S)
González, E. A. Prieto; Fuchs, A. G.; Sánchez, González S.
PUB. DATE
July 2009
SOURCE
Dose-Response;2009, Vol. 7 Issue 3, p234
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Amifostine [S-2-3-aminopropil amino ethyl phosphorotioic acid], a modulator agent for antineoplastic drugs involved in free radicals generation has given controversial results in cisplatin treated leukocytes in vitro. We have evaluated the amifostine protection over leukocytes in vivo, using comet assay. Groups of five OF1 male mice were given one of three doses of amifostine (56, 105 and 200 mg/Kg) after a cisplatin single injection (10 mg/Kg). Serum malonyldialdehide levels, catalase and superoxide dismutase activity were also evaluated. Amifostine showed significant DNA protection (p< 0.01) at the two lower doses evaluated. Malonyldildehide decreased in all amifostine treatments with respect to cisplatin while antioxidant enzyme activities remained unchanged. However, DNA migration increased with the highest amifostine dose; in fact highest dose of amifostine did no protect damage caused by cisplatin this result have implications on amifostine treatment schedules in clinical practice.
ACCESSION #
47331147

 

Related Articles

  • Protective Effect of Amifostine on High-Dose Methotrexate-Induced Small Intestinal Mucositis in Mice. Chen, Changying; Tian, Li; Zhang, Mingzhi; Sun, Qiaozhi; Zhang, Xudong; Li, Xiaodan; Cao, Xiaoqin; Liu, Qianqian; Li, Xiang; Hao, Li // Digestive Diseases & Sciences;Nov2013, Vol. 58 Issue 11, p3134 

    Background: Amifostine has been shown to be capable of minimizing radiotherapy-induced oral mucositis, but whether it protects small intestinal mucosae from high-dose methotrexate-induced damage is presently unknown. Aim: We aimed to evaluate the protective effect of amifostine against high-dose...

  • Assessing Cellular DNA Damage from A Helium Plasma Needle. Morales-Ramírez, P.; Cruz-Vallejo, V.; Peña-Eguiluz, R.; López-Callejas, R.; Rodríguez-Méndez, B. G.; Valencia-Alvarado, R.; Mercado-Cabrera, A.; Muñoz-Castro, A. E. // Radiation Research;Jun2013, Vol. 179 Issue 6, p669 

    The aim of the present study is to determine the deoxyribonucleic acid (DNA) damage by cells exposed to atmospheric pressure non-thermal plasma (APNTP). Mouse leukocytes embedded in agarose were exposed to the plasma at two different distances from a helium plasma needle outlet and during three...

  • Effects of thiolic antioxidants on in vitro mouse peritoneal macrophage functions. Pomaki, M.; Mota, J.; De La. Fuente, M.; Berger, J. // Comparative Clinical Pathology;Jul2005, Vol. 13 Issue 4, p176 

    Since leukocytes have the potential to produce oxygen free radicals that damage biomolecules, we have evaluated the influence of thiolic antioxidants (glutathione, N-acetylcysteine and thioproline) on selected hematoimmunological characteristics. We used peritoneal leukocytes incubated at 0.5,...

  • Cisplatin-induced pulse of germ cell apoptosis precedes long-term elevated apoptotic rates in C57/BL/6 mouse testis. Seaman, F.; Sawhney, P.; Giammona, C.J.; Richburg, J.H. // Apoptosis;Feb2003, Vol. 8 Issue 1, p101 

    Chemotherapeutic doses of cisplatin impair spermatogenesis and ultimately cause azoospermia and infertility in some men. The mechanism by which cisplatin damages testicular germ cells is poorly understood. Cisplatin's impact is first detected hours after exposure in the formation of DNA...

  • Chronic unpredictable stress exacerbates 7,12-dimethylbenz (a) anthracene induced hepatotoxicity and nephrotoxicity in Swiss albino mice. Suhail, Nida; Bilal, Nayeem; Hasan, Shirin; Banu, Naheed // Molecular & Cellular Biochemistry;Sep2011, Vol. 355 Issue 1/2, p117 

    Oxidative stress, a pervasive condition induced by stress has been implicated and recognized to be a prominent feature of various pathological states including cancer and their progression. The present study sought to validate the effectiveness of chronic unpredictable stress (CUS) on hepatic...

  • PROTECTIVE EFFECTS OF CURCUMIN IN CYCLOPHOSPHAMIDE INDUCED SPERM HEAD ABNORMALITIES IN MALE MICE. K. RUDRAMA DEV; K. YADAMMA; K. DILIP REDDY // International Journal of Pharma & Bio Sciences;Jan-Mar2013, Vol. 4 Issue 1, pB-1131 

    Natural dietary antioxidants are extensively studied for their ability to protect cells from damage to DNA induced by heavy metals. Two experiments were conducted to evaluate the protective effects of curcumin against cyclophosphamide induced cytogenetic damage in swiss albino mice, in the first...

  • Tannic acid mitigates cisplatin-induced nephrotoxicity in mice. Ahmad, S T; Sultana, S // Human & Experimental Toxicology;Feb2012, Vol. 31 Issue 2, p145 

    Cisplatin (CP) is a well-known chemotherapeutic drug that displays dose-limiting nephrotoxicity. In this study, tannic acid (TA), a naturally occurring plant polyphenol, was evaluated for its antioxidant and antigenotoxicity potential against the CP-induced renal oxidative stress and...

  • Potentiating effect of ferulic acid, a plant poyphenol on cisplatin in combination chemotherapy in dalton's ascites lymphoma mice model. Senapathy, Giftson J.; P., Umadevi // Journal of Pharmacy Research;Aug2011, Vol. 4 Issue 8, p2814 

    Due to the molecular complexity of many diseases, combination therapy is becoming increasingly important for a better long-term prognosis and to decrease side effects. 'Combination cancer chemotherapy' for the treatment of cancer generally refers to the administration of two or more...

  • Antioxidant and Protective Effects of Amrit Nectar Tablets on Adriamycin- and Cisplatin-Induced Toxicities. Dwivedi, Chandradhar; Agrawal, Puja; Natarajan, Karthika; Sharma, Hari // Journal of Alternative & Complementary Medicine;Feb2005, Vol. 11 Issue 1, p143 

    Maharishi herbal food supplements have been shown to inhibit the growth of mammary tumors and reduce free radical-mediated injuries. The purpose of this investigation is to evaluate the effects of aqueous and alcoholic extracts of Amrit Nectar tablets on rat liver microsomal lipid peroxidation...

Share

Read the Article

Courtesy of VIRGINIA BEACH PUBLIC LIBRARY AND SYSTEM

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics