A benchtop, ultrafast infrared spectroscopic imaging system for biomedical applications

Amrania, Hemmel; McCrow, Andrew; Phillips, Chris
December 2009
Review of Scientific Instruments;Dec2009, Vol. 80 Issue 12, p123702
Academic Journal
We discuss the potential biomedical applications for a novel infrared spectroscopic microimaging system. A tunable, table top solid-state laser has been coupled to a commercial infrared microscope, fitted with a modified high resolution infrared camera, to create a unique tool for midinfrared imaging. The system is capable of performing broadband imaging at a diffraction-limited spatial resolution, as is demonstrated here by spatially resolved spectroscopy of polymer test samples with a spectral resolution of 20 cm-1. The large pulse energies (tens of microjoules) offer previously unobtainable combinations of high signal-to-noise levels and rapid data collection times which are superior to current stand-alone laboratory instruments by many decades. Coupled with the short (100 ps) short pulse duration, these characteristics promise to make a wide range of time-resolved and reflection mode imaging experiments possible with live biological systems.


Related Articles

  • Preliminary Field Measurement of Cotton Fiber Micronaire by Portable NIR. Rodgers, James; Sho Yeung Kang; Fortier, Chanel; Xiaoliang Cui; Davidonis, Gayle; Clawson, Ernest; Boquet, Don; Pettigrew, William // Spectroscopy;Sep2010, Vol. 25 Issue 9, p38 

    The article discusses cotton fiber micronaire and portable near infrared (NIR) spectroscopy. It states that a program was carried out to expand the NIR measurement of cotton micronaire by portable NIR instrumentation form the laboratory to a non-laboratory location that is the cotton field. It...

  • Simple techniques for the generation of high peak power pulses with nanosecond and subnanosecond... Focia, R.J.; Schamiloglu, E.; Fleddermann, C.B. // Review of Scientific Instruments;Jul96, Vol. 67 Issue 7, p2626 

    Describes a method for generating high peak power pulses with nanosecond rise times. Use of a drift step recovery diode (DSRD) in an inductive-capacitive storage circuit; Method for sharpening the rise time of the pulses generated using DSRD to the subnanosecond range.

  • Ultrafast emission spectroscopy in the ultraviolet by time-gated upconversion. Kahlow, Michael A.; Jarzeba, Wl\odzimierz; DuBruil, Thomas P.; Barbara, Paul F. // Review of Scientific Instruments;Jul1988, Vol. 59 Issue 7, p1098 

    We have built a new apparatus to time resolve ultrafast fluorescence following ultraviolet excitation. A synchronously pumped dye laser produces optical pulses of 1-ps or 70-fs full-width half-maximum, depending upon dyes and optical configuration. These pulses are amplified at a 8.2-kHz...

  • Measurement of picosecond-pulse durations in the ultraviolet. Noordam, L. D.; ten Wolde, A.; van Linden van den Heuvell, H. B. // Review of Scientific Instruments;May89, Vol. 60 Issue 5, p835 

    A cross correlator for measurements of ultrashort UV pulses is described. The optical system is based on down-conversion of the UV pulses. The system can also be used to measure the second- and third-order autocorrelation function of pulses in the visible. The system is experimentally tested...

  • NIR Spectroscopy Takes Laboratory Equipment into the Field. Rzasa, Dave // Laboratory Equipment;Jan2008, Vol. 44 Issue 10, p28 

    The article features the near infrared (NIR) spectroscopy as a technology for detecting counterfeits, defective or suboptimal material composition in the U.S. Since spectroscopy is a method of measuring raw materials on large apparatus inside a laboratory, NIR spectroscopy offers a...

  • NIR integrating sphere.  // Spectroscopy;Apr2006, Vol. 21 Issue 4, p47 

    The article evaluates PIKE Technologies' near-infrared IntegratIR integrating sphere and provides information on design, options and key applications.

  • Near-infrared analyzers.  // Spectroscopy;Apr2006, Vol. 21 Issue 4, p48 

    The article evaluates some near-infrared analyzers from Thermo Electron and provides information on key features and applications.

  • Fourier Transform Infrared (FT-IR) Spectrometry. Sherma, Joseph; Larkin, John D.; Larkin, Frances H. // Journal of AOAC International;Jul/Aug2004, Vol. 87 Issue 4, p113A 

    Presents a field guide to Fourier Transform Infrared (FT-IR)-based spectrometry instrumentation. Description of the principle operation of FT-IR instruments to obtain a spectrum for analysis; Shift from radiation dispersion to interferometry and a processing circuit to perform Fourier analysis;...

  • Subpicosecond transient infrared spectroscopy of water: A theoretical description. Bratos, S.; Leicknam, J-Cl. // Journal of Chemical Physics;9/22/1995, Vol. 103 Issue 12, p4887 

    The pump–probe response of water in infrared is examined theoretically at time scales of the order of 100 fs; these times scales are characteristic of spectral diffusion. The theory is a statistical theory using correlation function description of the nonlinear optical processes involved....


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics