Ordered stretching of single molecules of deoxyribose nucleic acid between microfabricated polystyrene lines

Klein, D. C. G.; Gurevich, L.; Janssen, J. W.; Kouwenhoven, L. P.; Carbeck, J. D.; Sohn, L. L.
April 2001
Applied Physics Letters;4/16/2001, Vol. 78 Issue 16, p2396
Academic Journal
A technique for creating arrays of parallel, stretched single molecules of deoxyribose nucleic acid (DNA) on an arbitrary substrate for high-resolution scanning-probe imaging is discussed. The technique consists of lithographically patterning polystyrene lines on a substrate which then provide attachment sites for the ends of individual DNA molecules. Molecular combing is performed to stretch DNA from one polystyrene line to the other. Scanning-tunneling and atomic-force microscope images of single molecules of bacteriophage-lambda DNA are shown to demonstrate the advantages of this technique. Several applications, from high-resolution genomics to molecular electronics, are discussed. © 2001 American Institute of Physics.


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