Enhanced mucosal expression of interleukin-6 mRNA but not of interleukin-8 mRNA at the margin of gastric ulcer in Helicobacter pylori-positive gastritis

Furukawa, Koichi; Takahashi, Toru; Arai, Futoshi; Matsushima, Koji; Asakura, Hitoshi; Furukawa, K; Takahashi, T; Arai, F; Matsushima, K; Asakura, H
October 1998
Journal of Gastroenterology;1998, Vol. 33 Issue 5, p625
Academic Journal
journal article
To elucidate the role of interleukin (IL)-6 and IL-8 in the pathogenesis of gastric ulcer in Helicobacter pylori-positive gastritis, in situ hybridization using digoxigenin-labeled cDNA probes for both cytokines was performed. Immunogold silver staining was added to further improve the sensitivity of this non-radioactive hybridization. The biopsy specimens were taken from eight patients with active gastric ulcer before treatment, in all of whom H. pylori was positive. Macrophages (the putative producers of these cytokines) were determined by immunohistochemistry using anti-CD68 monoclonal antibodies (KP-1). IL-6 mRNA was most abundantly expressed in the epithelium and in the infiltrating cells in tissue adjacent to gastric ulcer. Quantitative analysis disclosed a significant increase in cells positive for IL-6 mRNA near the ulcer margin compared to cells in the surrounding tissue. In contrast, cells positive for IL-8 mRNA were observed in equal proportions and evenly in the epithelium and over the entire layer of the gastric mucosa regardless of the presence of gastric ulcer. The majority of infiltrating cells positive for both IL-6 and IL-8 mRNA were thought to be macrophages because of their morphologic features and their immunohistochemical reactivity to CD68. These findings strongly suggest that IL-6 is overexpressed at the margin of gastric ulcer in H. pylori-positive gastritis.


Related Articles

  • Toll-like Receptor 10 in Helicobacter pylori Infection. Hiroyuki Nagashima; Shun Iwatani; Cruz, Modesto; Abreu, José A. Jiménez; Tomohisa Uchida; Mahachai, Varocha; Vilaichone, Ratha-korn; Graham, David Y.; Yoshio Yamaoka; Nagashima, Hiroyuki; Iwatani, Shun; Jiménez Abreu, José A; Uchida, Tomohisa; Yamaoka, Yoshio // Journal of Infectious Diseases;11/15/2015, Vol. 212 Issue 10, p1666 

    Innate immunity plays important roles in the primary defense against pathogens, and epidemiological studies have suggested a role for Toll-like receptor 1 (TLR1) in Helicobacter pylori susceptibility. Microarray analysis of gastric biopsy specimens from H. pylori-positive and uninfected subjects...

  • Helicobacter pylori-Associated Gastric Ulcer Exhibits Enhanced Mucosal Chemokine Activity at the Ulcer Site. Shimizu, Tatsuji; Kusugami, Kazuo; Ina, Kenji; Imada, Akira; Nishio, Yuji; Hosokawa, Takehiko; Ohsuga, Masahiro; Shimada, Masaaki; Noshiro, Mitsuhiro; Kaneko, Hiroshi; Ando, Takafumi // Digestion;2000, Vol. 62 Issue 2/3, p87 

    Background and Aim: Although mucosal α- and β-chemokines are considered to be involved in the pathogenesis of Helicobacter pylori-associated gastritis, little is known how these chemokines are related to the ulcerogenesis in peptic ulcer patients. We examined the levels of interleukin...

  • The Importance of vacA, cagA, and iceA Genotypes of Helicobacter pylori Infection in Peptic Ulcer Disease and Gastroesophageal Reflux Disease. Arents, Nicolaas L. A.; Van Zwet, Anton A.; Thijs, Jacob C.; Kooistra-Smid, Anne M. D.; Van Slochteren, Kornelis R.; Degener, John E.; Kleibeuker, Jan H.; Van Doorn, Leen-Jan // American Journal of Gastroenterology (Springer Nature);Sep2001, Vol. 96 Issue 9, p2603 

    OBJECTIVE: To study the relationship between the presence of H. pylori virulence factors and clinical outcome in H. pylori infected patients. METHODS: DNA was isolated from an antral biopsy sample and vacA. cagA. and iceA genotype were determined by PCR and a reverse hybridization...

  • Current concepts in the management of Helicobacter pylon infection: the Maastricht III Consensus Report. Malfertheiner, P.; Megraud, F.; O'Morain, C.; Bazzoli, F.; El-Omar, E.; Graham, D.; Hunt, R.; Rokkas, I.; Vakil, N.; Kuipers, E. J. // Gut;Jun2007, Vol. 56 Issue 6, p772 

    Background: Guidelines on the management of Helicobacterpylori, which cover indications for management and treatment strategies, were produced in 2000. Aims: To update the guidelines at the European Helicobacter Study Group (EHSG) Third Maastricht Consensus Conference, with emphasis on the...

  • Elevated Interleukin-32 Expression Is Associated with Helicobacter pylori-Related Gastritis. Peng, Liu-sheng; Zhuang, Yuan; Li, Wen-hua; Zhou, Yuan-yuan; Wang, Ting-ting; Chen, Na; Cheng, Ping; Li, Bo-sheng; Guo, Hong; Yang, Shi-ming; Chen, Wei-san; Zou, Quan-ming // PLoS ONE;Mar2014, Vol. 9 Issue 3, p1 

    Background: Interleukin-32 (IL-32) is a recently discovered proinflammatory cytokine involved in inflammatory diseases. We investigated the expression of IL-32 and its regulation mechanism in the inflammatory response of patients with Helicobacter pylori (H. pylori) infection. Design and...

  • Helicobacter Genotyping and Detection in Peroperative Lavage Fluid in Patients with Perforated Peptic Ulcer. Komen, N. A. P.; Bertleff, M. J. O. E.; Van Doorn, L. J.; Lange, J. F.; De Graaf, P. W. // Journal of Gastrointestinal Surgery;Mar2008, Vol. 12 Issue 3, p555 

    Introduction and Objectives Certain Helicobacter pylori genotypes are associated with peptic ulcer disease; however, little is known about associations between the H. pylori genotype and perforated peptic ulcer (PPU). The primary aim of this study was to evaluate which genotypes are present in...

  • Detección del Gen cagA y Tipificación del Gen vacA en Cepas de Helicobacter pylori Aisladas de Pacientes con Enfermedad Úlcero- Péptica en Risaralda. Moncayo, José Ignacio; Santacruz, Jorge Javier // MedUNAB;ago2000, Vol. 3 Issue 8, p69 

    No abstract available.

  • Has Peptic Ulcer Disease Changed During the Past Ten Years in Korea? A Prospective Multi-center Study. Hyun Jang; Min Choi; Woon Shin; Kyung Kim; Yong Chung; Kyoung Kim; Cheol Park; Il Baek; Kwang Baik; Sea Kae; Hak Kim // Digestive Diseases & Sciences;Jun2008, Vol. 53 Issue 6, p1527 

    Abstract   Background The incidence of H. pylori-negative, idiopathic peptic ulcer disease (IPUD) seems to be increasing with the changing trends of PUD and H. pylori infection in some developed countries. Aim To investigate the changing trend of PUD and the prevalence of H. pylori...

  • Specific and Sensitive Detection of H. pylori in Biological Specimens by Real-Time RT-PCR and In Situ Hybridization. Hui Liu; Rahman, Arifur; Semino-Mora, Cristina; Doi, Sonia Q.; Dubois, Andre // PLoS ONE;2008, Vol. 3 Issue 7, p1 

    PCR detection of H. pylori in biological specimens is rendered difficult by the extensive polymorphism of H. pylori genes and the suppressed expression of some genes in many strains. The goal of the present study was to (1) define a domain of the 16S rRNA sequence that is both highly conserved...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics