Colonization of microflora in mice: mucosal defense against luminal bacteria

Fukushima, Kouhei; Sasaki, Iwao; Ogawa, Hitoshi; Naito, Hiroo; Funayama, Yuji; Matsuno, Seiki; Fukushima, K; Sasaki, I; Ogawa, H; Naito, H; Funayama, Y; Matsuno, S
February 1999
Journal of Gastroenterology;1999, Vol. 34 Issue 1, p54
Academic Journal
journal article
To investigate the pathogenesis of inflammatory bowel disease, it is critical to develop a system that uses simple and reproducible models for analyzing the "normal" mucosal defense mechanism. In the present study, germ-free mice were conventionalized by the oral administration of microorganisms prepared from the feces of genetically identical mice. Histological assessment and mucin characterization of small intestine and colon were then carried out. Histological findings in the gut were site-dependent and clearly time-dependent. Acute inflammation was most evident in the cecum. The cecal mucosa exhibited hyperplastic changes in epithelial cells, infiltration of polymorphonuclear cells, crypt abscesses, and epithelial projections on the epithelial surface 7 days after conventionalization. Some of the changes were similar to those seen in human ulcerative colitis. The histological findings in the conventionalized mice were comparable to those in specific pathogen-free mice after 28 days. Mucin histochemistry revealed that bacterial colonization altered the number of rectal goblet cells and the mucin composition in a time-dependent fashion. Although this model shares only some characteristics of human inflammatory bowel disease, it is unique in demonstrating the acquisition of mucosal defense. Understanding of this process is critical for the elucidation of inflammatory bowel disease pathogenesis.


Related Articles

  • Overproduction of Outer Membrane Protein A by Acinetobacter baumannii as a Risk Factor for Nosocomial Pneumonia, Bacteremia, and Mortality Rate Increase.  // Journal of Infectious Diseases;3/15/2017, Vol. 215 Issue 6, p966 

    Background: Outer membrane protein A (OmpA) is a porin involved in Acinetobacter baumannii pathogenesis. However, OmpA clinical implication in hospital-acquired infections remains unknown. We aimed to determine whether OmpA overproduction was a risk factor associated with pneumonia,...

  • The cytomegalovirus-encoded chemokine receptor US28 promotes intestinal neoplasia in transgenic mice. Bongers, Gerold; Maussang, David; Muniz, Luciana R.; Noriega, Vanessa M.; Fraile-Ramos, Alberto; Barker, Nick; Marchesi, Federica; Thirunarayanan, Nanthakumar; Vischer, Henry F.; Lihui Qin; Mayer, Lloyd; Harpaz, Noam; Leurs, Rob; Furtado, Glaucia C.; Clevers, Hans; Tortorella, Domenico; Smit, Martine J.; Lira, Sergio A.; Qin, Lihui // Journal of Clinical Investigation;Nov2010, Vol. 120 Issue 11, p3969 

    US28 is a constitutively active chemokine receptor encoded by CMV (also referred to as human herpesvirus 5), a highly prevalent human virus that infects a broad spectrum of cells, including intestinal epithelial cells (IECs). To study the role of US28 in vivo, we created transgenic mice (VS28...

  • Heparanase powers a chronic inflammatory circuit that promotes colitis-associated tumorigenesis in mice. Lerner, Immanuel; Hermano, Esther; Zcharia, Eyal; Rodkin, Dina; Bulvik, Raanan; Doviner, Victoria; Rubinstein, Ariel M.; Ishai-Michaeli, Rivka; Atzmon, Ruth; Sherman, Yoav; Meirovitz, Amichay; Peretz, Tamar; Vlodavsky, Israel; Elkin, Michael // Journal of Clinical Investigation;May2011, Vol. 121 Issue 5, p1709 

    Ulcerative colitis (UC) is a chronic inflammatory bowel disease that is closely associated with colon cancer. Expression of the enzyme heparanase is clearly linked to colon carcinoma progression, but its role in UC is unknown. Here we demonstrate for what we believe to be the first time the...

  • Prostaglandin E2 reduces radiation-induced epithelial apoptosis through a mechanism involving AKT activation and bax translocation. Tessner, Teresa G.; Muhale, Filipe; Riehl, Terrence E.; Anant, Shrikant; Stenson, William F. // Journal of Clinical Investigation;Dec2004, Vol. 114 Issue 11, p1676 

    Prostaglandin E2 (PGE2) synthesis modulates the response to radiation injury in the mouse intestinal epithelium through effects on crypt survival and apoptosis; however, the downstream signaling events have not been elucidated. WT mice receiving 16,16-dimethyl PGE2 (dmPGE2) had fewer apoptotic...

  • Estimation of the total parasite biomass in acute falciparum malaria from plasma PfHRP2. Dondorp, Arjen M; Desakorn, Varunee; Pongtavornpinyo, Wirichada; Sahassananda, Duangjai; Silamut, Kamolrat; Chotivanich, Kesinee; Newton, Paul N; Pitisuttithum, Punnee; Smithyman, A M; White, Nicholas J; Day, Nicholas P J // PLoS Medicine;Aug2005, Vol. 2 Issue 8, pe204 

    Background: In falciparum malaria sequestration of erythrocytes containing mature forms of Plasmodium falciparum in the microvasculature of vital organs is central to pathology, but quantitation of this hidden sequestered parasite load in vivo has not previously been possible. The...

  • Bacteria used for the production of yogurt inactivate carcinogens and prevent DNA damage in the colon of rats. Wollowski, Ingrid; Ji, Seung-Taek; Wollowski, I; Ji, S T; Bakalinsky, A T; Neudecker, C; Pool-Zobel, B L // Journal of Nutrition;Jan99, Vol. 129 Issue 1, p77 

    Lactic acid-producing bacteria prevent carcinogen-induced preneoplastic lesions and tumors in rat colon. Because the mechanisms responsible for these protective effects are unknown, two strains of lactic acid bacteria, Lactobacillus delbrueckii ssp. bulgaricus 191R and Streptococcus salivarius...

  • Frailty and Subsequent Disability and Mortality among Patients with Critical Illness. Brummel, Nathan E.; Bell, Susan P.; Girard, Timothy D.; Pandharipande, Pratik P.; Jackson, James C.; Morandi, Alessandro; Thompson, Jennifer L.; Chandrasekhar, Rameela; Bernard, Gordon R.; Dittus, Robert S.; Gill, Thomas M.; Ely, E. Wesley // American Journal of Respiratory & Critical Care Medicine;7/1/2017, Vol. 196 Issue 1, p64 

    Rationale: The prevalence of frailty (diminished physiologic reserve) and its effect on outcomes for those aged 18 years and older with critical illness is unclear.Objectives: We hypothesized greater frailty would be associated with subsequent mortality, disability, and...

  • Prospective, Randomized Study on the Efficacy and Safety of Local UV-Free Blue Light Treatment of Eczema. Keemss, Kim; Pfaff, Stephanie C.; Born, Matthias; Liebmann, Jörg; Merk, Hans F.; von Felbert, Verena // Dermatology (10188665);Sep2016, Vol. 232 Issue 4, p496 

    Background: Blue light was shown to reduce the activation of T cells and modulate cytokine release in vitro. Therefore, we investigated the efficacy of blue light in the treatment of eczema.Methods: A sample of 21 patients with mild to moderate eczema were locally...

  • Defining secondary progressive multiple sclerosis. Lorscheider, Johannes; Buzzard, Katherine; Jokubaitis, Vilija; Spelman, Tim; Havrdova, Eva; Horakova, Dana; Trojano, Maria; Izquierdo, Guillermo; Girard, Marc; Duquette, Pierre; Prat, Alexandre; Lugaresi, Alessandra; Grand'Maison, François; Grammond, Pierre; Hupperts, Raymond; Alroughani, Raed; Sola, Patrizia; Boz, Cavit; Pucci, Eugenio; Lechner-Scott, Jeanette // Brain: A Journal of Neurology;9/1/2016, Vol. 139 Issue 9, p2395 

    A number of studies have been conducted with the onset of secondary progressive multiple sclerosis as an inclusion criterion or an outcome of interest. However, a standardized objective definition of secondary progressive multiple sclerosis has been lacking. The aim of this work was to evaluate...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics