TITLE

Nicotine-induced CCN2: from Smoking to Periodontal Fibrosis

AUTHOR(S)
Takeuchi, H.; Kubota, S.; Murakashi, E.; Zhou, Y.; Endo, K.; Ng, P. S.; Takigawa, M.; Numabe, Y.
PUB. DATE
January 2010
SOURCE
Journal of Dental Research;Jan2010, Vol. 89 Issue 1, p34
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Since fibrosis is observed in smokers' gingiva, it was hypothesized that fibrosis was caused by nicotine in the periodontium. Therefore, in this study, we investigated the effects of nicotine on the induction of a profibrotic molecule, connective tissue growth factor (CCN2/CTGF), in human gingival fibroblasts (HGFs) and periodontal ligament (PDL) cells. With 1 µg/mL nicotine, vacuolization and attenuated proliferation were observed. Interestingly, 1 µg/mL nicotine increased the production of CCN2/CTGF protein in both cells without increasing mRNA expression. Furthermore, type I collagen mRNA and protein were also increased and were significantly blocked by a CCN2/CTGF neutralizing antibody. This is the first report to describe a relationship between nicotine and CCN2/CTGF in periodontal tissue cells. Analysis of our data also indicated that nicotine was cytotoxic, while it increased CCN2/CTGF and, eventually, type I collagen production. These findings suggest that periodontal fibrosis can be promoted by nicotine from smoking via effects on CCN2/CTGF.
ACCESSION #
46709300

 

Related Articles

  • Cigarette smoking induces atrial fibrosis in humans via nicotine. Goette, Andreas; Lendeckel, Uwe; Kuchenbecker, Anja; Bukowska, Alicja; Peters, Brigitte; Klein, Helmut U.; Huth, Christof; Röcken, Christoph // Heart;Sep2007, Vol. 93 Issue 9, p1056 

    Background: Cigarette smoking (CS) promotes endothelial dysfunction and atherosclerosis in the vascular bed. The impact of smoking on atrial myocardium is not defined in humans. Objective: To determine the effect of CS on the development of interstitial fibrosis in atrial myocardium. Design:...

  • Expression of Transforming Growth Factor-beta 1 (TGF-β1) in the Developing Periodontium of Rats. Gao, J.; Symons, A. L.; Bartold, P. M. // Journal of Dental Research;Sep1998, Vol. 77 Issue 9, p1708 

    Transforming growth factor-beta 1 (TGF-β1) has been reported to be expressed within several tissue compartments of developing molar crowns and therefore is implicated in tooth development. Additionally, TGF-β1 may also play a crucial role in tissue repair and regeneration. The aim of this...

  • Nicotine Lozenges.  // AHFS Consumer Medication Information;Sep2020, p1 

    Nicotine lozenges are used to help people stop smoking. Nicotine lozenges are in a class of medications called smoking cessation aids. They work by providing nicotine to your body to decrease the withdrawal symptoms experienced when smoking is stopped and to reduce the urge to smoke.

  • The Experimental Autoimmune Encephalomyelitis Disease Course Is Modulated by Nicotine and Other Cigarette Smoke Components. Gao, Zhen; Nissen, Jillian C.; Ji, Kyungmin; Tsirka, Stella E. // PLoS ONE;Sep2014, Vol. 9 Issue 9, p1 

    Epidemiological studies have reported that cigarette smoking increases the risk of developing multiple sclerosis (MS) and accelerates its progression. However, the molecular mechanisms underlying these effects remain unsettled. We have investigated here the effects of the nicotine and the...

  • Vascular endothelial growth factor (VEGF) levels of gingiva and gingival crevicular fluid in diabetic and systemically healthy periodontitis patients. Sakallıoğlu, Elif Eser; Aliyev, Eldar; Lütfioğlu, Müge; Yavuz, Ümit; Açıkgöz, Gökhan // Clinical Oral Investigations;Jun2007, Vol. 11 Issue 2, p115 

    It has been demonstrated that diabetes mellitus (DM) may have an inductive effect on the vascular endothelial growth factor (VEGF) levels of periodontium during periodontal disease. The aim of this study is to confirm this phenomenon, investigating whether it is also valid for diabetic...

  • Increased expression of decorin in experimental hydronephrosis. Diamond, Jonathan R.; Levinson, Mark; Kreisberg, Robert; Ricardo, Sharon D. // Kidney International;Apr1997, Vol. 51 Issue 4, p1133 

    Transforming growth factor (TGF)-β1 is a potential mediator of tubulointerstitial (TI) fibrosis in the rat unilateral ureteral obstruction (UUO) model. Decorin is a protein composed of a core protein and a chondroitin sulfate side chain and is capable of inactivating TGF-β. Since TGF-β...

  • Postnatal development of neural chemoafferent pathway and respiration is altered following prenatal nicotine exposure in rats. Malhière, S.; Dalmaz, Y.; Perrin, D.; Roux, J. C.; Lagercrantz, H.; Pequignot, J. M.; Peyronnet, J. // Respiratory Research;2001 Supplement 1, Vol. 2, pP16 

    An abstract of the study "Postnatal development of neural chemoafferent pathway and respiration is altered following prenatal nicotine exposure in rats," by S. Malhiere and colleagues is presented.

  • Prenatal nicotine exposure increases apnoea and attenuates nicotinic potentiation of hypoglossal output in mice. Robinson, D. M.; Adams, B. M.; Kwok, H.; Peebles, K. C.; Clarke, L. L.; Funk, G. D. // Respiratory Research;2001 Supplement 1, Vol. 2, pP17 

    An abstract of the study "Prenatal nicotine exposure increases apnoea and attenuates nicotinic potentiation of hypoglossal output in mice," by D.M. Robinson and colleagues.

  • Nicotina y enfermedad periodontal. Antúnez Treviño, Jorge M.; Gutiérrez Jáuregui, Nelda; Flores García, Gabriela S.; Cárdenas Franco, Carolina; Treviño Alanís, Ma Guadalupe; Martínez Menchaca, Héctor R.; Rivera Silva, Gerardo // Revista ADM;Nov/Dic2013, Vol. 70 Issue 6, p292 

    Nicotine is a substance that directly damages the periodontium at both a cellular and molecular level, thus furthering the advance of periodontal disease. It can cause different degrees of damage and affects all periodontal tissues, such as periodontal ligament, alveolar bone, dental cement, and...

Share

Read the Article

Courtesy of THE LIBRARY OF VIRGINIA

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics